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Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

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ABSTRACT

In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.

No MeSH data available.


Functional classification and subcellular localization of DAPs from leaves and roots.(A) Functional classification; (B) Subcellular localization. Cyto, Cytoplasm; Cysk, Cytoskeleton; ER, Endoplasmic Reticulum; Extr, Extracellular; Mito, Mitochondrial; Nucl, Nuclear
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f5: Functional classification and subcellular localization of DAPs from leaves and roots.(A) Functional classification; (B) Subcellular localization. Cyto, Cytoplasm; Cysk, Cytoskeleton; ER, Endoplasmic Reticulum; Extr, Extracellular; Mito, Mitochondrial; Nucl, Nuclear

Mentions: All 176 DAPs identified in roots and leaves were classified by function into six categories (Fig. 5A): signal transduction, energy metabolism, detoxification and stress responses, protein metabolism, cell wall and structure, and other functions. The energy metabolism category was the largest and included 25 (32.05%) and 57 (58.16%) DAPs from roots and leaves, respectively. Detoxification and stress response proteins accounted for 20.51% (roots) and 5.1% (leaves), while DAPs involved in protein metabolism accounted for 16.67 and 18.37% from roots and leaves, respectively. Proteins associated with the cell wall/structure comprised 7.69% of those in roots and 3.06% of those in leaves. Only four and one DAP identified from roots and leaves, respectively were related to signal transduction.


Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery
Functional classification and subcellular localization of DAPs from leaves and roots.(A) Functional classification; (B) Subcellular localization. Cyto, Cytoplasm; Cysk, Cytoskeleton; ER, Endoplasmic Reticulum; Extr, Extracellular; Mito, Mitochondrial; Nucl, Nuclear
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384013&req=5

f5: Functional classification and subcellular localization of DAPs from leaves and roots.(A) Functional classification; (B) Subcellular localization. Cyto, Cytoplasm; Cysk, Cytoskeleton; ER, Endoplasmic Reticulum; Extr, Extracellular; Mito, Mitochondrial; Nucl, Nuclear
Mentions: All 176 DAPs identified in roots and leaves were classified by function into six categories (Fig. 5A): signal transduction, energy metabolism, detoxification and stress responses, protein metabolism, cell wall and structure, and other functions. The energy metabolism category was the largest and included 25 (32.05%) and 57 (58.16%) DAPs from roots and leaves, respectively. Detoxification and stress response proteins accounted for 20.51% (roots) and 5.1% (leaves), while DAPs involved in protein metabolism accounted for 16.67 and 18.37% from roots and leaves, respectively. Proteins associated with the cell wall/structure comprised 7.69% of those in roots and 3.06% of those in leaves. Only four and one DAP identified from roots and leaves, respectively were related to signal transduction.

View Article: PubMed Central - PubMed

ABSTRACT

In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.

No MeSH data available.