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Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

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ABSTRACT

In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.

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Venn diagram analysis of DAP spots in Bd21 roots and leaves under drought stress and recovery.(A) Venn diagram analysis of DAP spots in roots and leaves; (B) Venn diagram analysis of DAP spots under under drought stress and recovery. The red number represents the number of unique protein species identified.
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f3: Venn diagram analysis of DAP spots in Bd21 roots and leaves under drought stress and recovery.(A) Venn diagram analysis of DAP spots in roots and leaves; (B) Venn diagram analysis of DAP spots under under drought stress and recovery. The red number represents the number of unique protein species identified.

Mentions: The number of DAP spots and their overlapping relationships following drought treatment and recovery are shown as Venn diagrams in Fig. 3A. In roots, 47 DAP spots exhibited significantly changed abundance in response to drought stress. This was many more than the 25 DAP spots that were differentially accumulated during recovery in roots, and similar to the number of DAP spots (95) that accumulated differentially during recovery in leaves. These results suggest that the drought response of plants continues during the subsequent recovery periods. Under drought stress, the numbers of DAP spots in roots and leaves were similar, but when the drought treatment was removed there were far more DAP spots in leaves (75) than in roots (54), we speculate that during recovery, metabolic activity in leaves was greater than in roots. An analysis using Venn diagrams of unique protein species identified under drought stress and during the subsequent recovery period provided similar results (Fig. 3B).


Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery
Venn diagram analysis of DAP spots in Bd21 roots and leaves under drought stress and recovery.(A) Venn diagram analysis of DAP spots in roots and leaves; (B) Venn diagram analysis of DAP spots under under drought stress and recovery. The red number represents the number of unique protein species identified.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384013&req=5

f3: Venn diagram analysis of DAP spots in Bd21 roots and leaves under drought stress and recovery.(A) Venn diagram analysis of DAP spots in roots and leaves; (B) Venn diagram analysis of DAP spots under under drought stress and recovery. The red number represents the number of unique protein species identified.
Mentions: The number of DAP spots and their overlapping relationships following drought treatment and recovery are shown as Venn diagrams in Fig. 3A. In roots, 47 DAP spots exhibited significantly changed abundance in response to drought stress. This was many more than the 25 DAP spots that were differentially accumulated during recovery in roots, and similar to the number of DAP spots (95) that accumulated differentially during recovery in leaves. These results suggest that the drought response of plants continues during the subsequent recovery periods. Under drought stress, the numbers of DAP spots in roots and leaves were similar, but when the drought treatment was removed there were far more DAP spots in leaves (75) than in roots (54), we speculate that during recovery, metabolic activity in leaves was greater than in roots. An analysis using Venn diagrams of unique protein species identified under drought stress and during the subsequent recovery period provided similar results (Fig. 3B).

View Article: PubMed Central - PubMed

ABSTRACT

In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.

No MeSH data available.


Related in: MedlinePlus