Limits...
Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

View Article: PubMed Central - PubMed

ABSTRACT

In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.

No MeSH data available.


Related in: MedlinePlus

2D-DIGE images of Bd21 roots and leaves under drought stress and recovery.(A) Leaf gel 1; (B) Leaf gel 4; (C) Root gel 1; (D) Root gel 4. Numbered lines indicate spots that were identified by MALDI-TOF/TOF-MS and significantly regulated under drought stress and recovery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5384013&req=5

f2: 2D-DIGE images of Bd21 roots and leaves under drought stress and recovery.(A) Leaf gel 1; (B) Leaf gel 4; (C) Root gel 1; (D) Root gel 4. Numbered lines indicate spots that were identified by MALDI-TOF/TOF-MS and significantly regulated under drought stress and recovery.

Mentions: Two-dimensional difference gel electrophoresis (2D-DIGE) demonstrated that changes in 143 and 149 protein species had occurred at 24 h and 24 hR in roots and leaves, respectively (Fig. 2). We detected the dynamic expression profiles of these differentially accumulated proteins at 0, 6, 12, 24, and 48 h, and 6, 12, 24, and 48 hR (DAPs) using two-dimensional electrophoresis (2-DE). All of the DAP spots were reproducibly detected and matched using the 2-DE profiles from roots and leaves, respectively. Representative 2D-DIGE and 2-DE gels are shown in Fig. 2 and in Supplementary Figs S2 and S3.


Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery
2D-DIGE images of Bd21 roots and leaves under drought stress and recovery.(A) Leaf gel 1; (B) Leaf gel 4; (C) Root gel 1; (D) Root gel 4. Numbered lines indicate spots that were identified by MALDI-TOF/TOF-MS and significantly regulated under drought stress and recovery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384013&req=5

f2: 2D-DIGE images of Bd21 roots and leaves under drought stress and recovery.(A) Leaf gel 1; (B) Leaf gel 4; (C) Root gel 1; (D) Root gel 4. Numbered lines indicate spots that were identified by MALDI-TOF/TOF-MS and significantly regulated under drought stress and recovery.
Mentions: Two-dimensional difference gel electrophoresis (2D-DIGE) demonstrated that changes in 143 and 149 protein species had occurred at 24 h and 24 hR in roots and leaves, respectively (Fig. 2). We detected the dynamic expression profiles of these differentially accumulated proteins at 0, 6, 12, 24, and 48 h, and 6, 12, 24, and 48 hR (DAPs) using two-dimensional electrophoresis (2-DE). All of the DAP spots were reproducibly detected and matched using the 2-DE profiles from roots and leaves, respectively. Representative 2D-DIGE and 2-DE gels are shown in Fig. 2 and in Supplementary Figs S2 and S3.

View Article: PubMed Central - PubMed

ABSTRACT

In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.

No MeSH data available.


Related in: MedlinePlus