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Characterization and genomic analyses of two newly isolated Morganella phages define distant members among Tevenvirinae and Autographivirinae subfamilies

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ABSTRACT

Morganella morganii is a common but frequent neglected environmental opportunistic pathogen which can cause deadly nosocomial infections. The increased number of multidrug-resistant M. morganii isolates motivates the search for alternative and effective antibacterials. We have isolated two novel obligatorily lytic M. morganii bacteriophages (vB_MmoM_MP1, vB_MmoP_MP2) and characterized them with respect to specificity, morphology, genome organization and phylogenetic relationships. MP1’s dsDNA genome consists of 163,095 bp and encodes 271 proteins, exhibiting low DNA (<40%) and protein (<70%) homology to other members of the Tevenvirinae. Its unique property is a >10 kb chromosomal inversion that encompass the baseplate assembly and head outer capsid synthesis genes when compared to other T-even bacteriophages. MP2 has a dsDNA molecule with 39,394 bp and encodes 55 proteins, presenting significant genomic (70%) and proteomic identity (86%) but only to Morganella bacteriophage MmP1. MP1 and MP2 are then novel members of Tevenvirinae and Autographivirinae, respectively, but differ significantly from other tailed bacteriophages of these subfamilies to warrant proposing new genera. Both bacteriophages together could propagate in 23 of 27 M. morganii clinical isolates of different origin and antibiotic resistance profiles, making them suitable for further studies on a development of bacteriophage cocktail for potential therapeutic applications.

No MeSH data available.


Divergence of the core gene organization of Morganella phages genomes.Pairwise comparisons of (A) MP1 and (B) MP2 genomes using tbBLASTX within EasyFig against the closest homolog (Pm5461 or MmP1) and the phage archetypes (T4 or T7), respectively. Arrows indicating CDSs are drawn to scale and colored in the reference genome (MP1 or MP2) according to their predicted function. Gene similarity profiles between phages are indicated in grayscale (and percentage). Major diversions are marked in roman numbers.
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f7: Divergence of the core gene organization of Morganella phages genomes.Pairwise comparisons of (A) MP1 and (B) MP2 genomes using tbBLASTX within EasyFig against the closest homolog (Pm5461 or MmP1) and the phage archetypes (T4 or T7), respectively. Arrows indicating CDSs are drawn to scale and colored in the reference genome (MP1 or MP2) according to their predicted function. Gene similarity profiles between phages are indicated in grayscale (and percentage). Major diversions are marked in roman numbers.

Mentions: To individualize the similarities and differences of MP1 and MP2, we constructed comparative genome maps to show the detail diversion of the core gene organisation against phages with closer homology (Fig. 7). The starting gene and orientation of MP1 and MP2 followed the T4 and T7 convention, respectively.


Characterization and genomic analyses of two newly isolated Morganella phages define distant members among Tevenvirinae and Autographivirinae subfamilies
Divergence of the core gene organization of Morganella phages genomes.Pairwise comparisons of (A) MP1 and (B) MP2 genomes using tbBLASTX within EasyFig against the closest homolog (Pm5461 or MmP1) and the phage archetypes (T4 or T7), respectively. Arrows indicating CDSs are drawn to scale and colored in the reference genome (MP1 or MP2) according to their predicted function. Gene similarity profiles between phages are indicated in grayscale (and percentage). Major diversions are marked in roman numbers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384007&req=5

f7: Divergence of the core gene organization of Morganella phages genomes.Pairwise comparisons of (A) MP1 and (B) MP2 genomes using tbBLASTX within EasyFig against the closest homolog (Pm5461 or MmP1) and the phage archetypes (T4 or T7), respectively. Arrows indicating CDSs are drawn to scale and colored in the reference genome (MP1 or MP2) according to their predicted function. Gene similarity profiles between phages are indicated in grayscale (and percentage). Major diversions are marked in roman numbers.
Mentions: To individualize the similarities and differences of MP1 and MP2, we constructed comparative genome maps to show the detail diversion of the core gene organisation against phages with closer homology (Fig. 7). The starting gene and orientation of MP1 and MP2 followed the T4 and T7 convention, respectively.

View Article: PubMed Central - PubMed

ABSTRACT

Morganella morganii is a common but frequent neglected environmental opportunistic pathogen which can cause deadly nosocomial infections. The increased number of multidrug-resistant M. morganii isolates motivates the search for alternative and effective antibacterials. We have isolated two novel obligatorily lytic M. morganii bacteriophages (vB_MmoM_MP1, vB_MmoP_MP2) and characterized them with respect to specificity, morphology, genome organization and phylogenetic relationships. MP1’s dsDNA genome consists of 163,095 bp and encodes 271 proteins, exhibiting low DNA (<40%) and protein (<70%) homology to other members of the Tevenvirinae. Its unique property is a >10 kb chromosomal inversion that encompass the baseplate assembly and head outer capsid synthesis genes when compared to other T-even bacteriophages. MP2 has a dsDNA molecule with 39,394 bp and encodes 55 proteins, presenting significant genomic (70%) and proteomic identity (86%) but only to Morganella bacteriophage MmP1. MP1 and MP2 are then novel members of Tevenvirinae and Autographivirinae, respectively, but differ significantly from other tailed bacteriophages of these subfamilies to warrant proposing new genera. Both bacteriophages together could propagate in 23 of 27 M. morganii clinical isolates of different origin and antibiotic resistance profiles, making them suitable for further studies on a development of bacteriophage cocktail for potential therapeutic applications.

No MeSH data available.