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Characterization and genomic analyses of two newly isolated Morganella phages define distant members among Tevenvirinae and Autographivirinae subfamilies

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ABSTRACT

Morganella morganii is a common but frequent neglected environmental opportunistic pathogen which can cause deadly nosocomial infections. The increased number of multidrug-resistant M. morganii isolates motivates the search for alternative and effective antibacterials. We have isolated two novel obligatorily lytic M. morganii bacteriophages (vB_MmoM_MP1, vB_MmoP_MP2) and characterized them with respect to specificity, morphology, genome organization and phylogenetic relationships. MP1’s dsDNA genome consists of 163,095 bp and encodes 271 proteins, exhibiting low DNA (<40%) and protein (<70%) homology to other members of the Tevenvirinae. Its unique property is a >10 kb chromosomal inversion that encompass the baseplate assembly and head outer capsid synthesis genes when compared to other T-even bacteriophages. MP2 has a dsDNA molecule with 39,394 bp and encodes 55 proteins, presenting significant genomic (70%) and proteomic identity (86%) but only to Morganella bacteriophage MmP1. MP1 and MP2 are then novel members of Tevenvirinae and Autographivirinae, respectively, but differ significantly from other tailed bacteriophages of these subfamilies to warrant proposing new genera. Both bacteriophages together could propagate in 23 of 27 M. morganii clinical isolates of different origin and antibiotic resistance profiles, making them suitable for further studies on a development of bacteriophage cocktail for potential therapeutic applications.

No MeSH data available.


Morphology and genome overview of MP1.(A) Electron micrographs of Morganella-infecting phage MP1 negatively stained with 2% uranyl acetate. (B) Genome map with predicted 271 CDSs numbered and coloured (yellow, green, blue and gray) according to their predicted function. Protein identified by mass spectrometry are pointed with vertical arrows. Above genome, the nucleotide positions in kb are given.
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f1: Morphology and genome overview of MP1.(A) Electron micrographs of Morganella-infecting phage MP1 negatively stained with 2% uranyl acetate. (B) Genome map with predicted 271 CDSs numbered and coloured (yellow, green, blue and gray) according to their predicted function. Protein identified by mass spectrometry are pointed with vertical arrows. Above genome, the nucleotide positions in kb are given.

Mentions: MP1 produces uniformly small plaques (0.1-mm diameter), which are difficult to enumerate even in a bacterial lawn solidified with 0.4% agar. MP2 always forms small (0.5-mm diameter) clear and large (2-mm diameter) plaque variants, both surrounded by haloes for each Morganella strain tested. The presence of opaque halos suggests active production of exopolysaccharide depolymerase-like enzymes48, which have never been described for Morganella phages. Transmission electron micrographs revealed two morphotypes (Figs 1A and 2A). MP1 is a myovirus with an elongated icosahedral head (85 nm in length and 65 nm in width) and a contractile tail of 111 nm long and 17 nm wide. MP2 has a smaller isometric head between 48 and 51 nm in diameter and a short (7 nm) non-contractile tail, which is a feature common to members of the Podoviridae4. MP1 and MP2 are then morphologically similar to Morganella phages FSP1 and MmP1, respectively.


Characterization and genomic analyses of two newly isolated Morganella phages define distant members among Tevenvirinae and Autographivirinae subfamilies
Morphology and genome overview of MP1.(A) Electron micrographs of Morganella-infecting phage MP1 negatively stained with 2% uranyl acetate. (B) Genome map with predicted 271 CDSs numbered and coloured (yellow, green, blue and gray) according to their predicted function. Protein identified by mass spectrometry are pointed with vertical arrows. Above genome, the nucleotide positions in kb are given.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5384007&req=5

f1: Morphology and genome overview of MP1.(A) Electron micrographs of Morganella-infecting phage MP1 negatively stained with 2% uranyl acetate. (B) Genome map with predicted 271 CDSs numbered and coloured (yellow, green, blue and gray) according to their predicted function. Protein identified by mass spectrometry are pointed with vertical arrows. Above genome, the nucleotide positions in kb are given.
Mentions: MP1 produces uniformly small plaques (0.1-mm diameter), which are difficult to enumerate even in a bacterial lawn solidified with 0.4% agar. MP2 always forms small (0.5-mm diameter) clear and large (2-mm diameter) plaque variants, both surrounded by haloes for each Morganella strain tested. The presence of opaque halos suggests active production of exopolysaccharide depolymerase-like enzymes48, which have never been described for Morganella phages. Transmission electron micrographs revealed two morphotypes (Figs 1A and 2A). MP1 is a myovirus with an elongated icosahedral head (85 nm in length and 65 nm in width) and a contractile tail of 111 nm long and 17 nm wide. MP2 has a smaller isometric head between 48 and 51 nm in diameter and a short (7 nm) non-contractile tail, which is a feature common to members of the Podoviridae4. MP1 and MP2 are then morphologically similar to Morganella phages FSP1 and MmP1, respectively.

View Article: PubMed Central - PubMed

ABSTRACT

Morganella morganii is a common but frequent neglected environmental opportunistic pathogen which can cause deadly nosocomial infections. The increased number of multidrug-resistant M. morganii isolates motivates the search for alternative and effective antibacterials. We have isolated two novel obligatorily lytic M. morganii bacteriophages (vB_MmoM_MP1, vB_MmoP_MP2) and characterized them with respect to specificity, morphology, genome organization and phylogenetic relationships. MP1’s dsDNA genome consists of 163,095 bp and encodes 271 proteins, exhibiting low DNA (<40%) and protein (<70%) homology to other members of the Tevenvirinae. Its unique property is a >10 kb chromosomal inversion that encompass the baseplate assembly and head outer capsid synthesis genes when compared to other T-even bacteriophages. MP2 has a dsDNA molecule with 39,394 bp and encodes 55 proteins, presenting significant genomic (70%) and proteomic identity (86%) but only to Morganella bacteriophage MmP1. MP1 and MP2 are then novel members of Tevenvirinae and Autographivirinae, respectively, but differ significantly from other tailed bacteriophages of these subfamilies to warrant proposing new genera. Both bacteriophages together could propagate in 23 of 27 M. morganii clinical isolates of different origin and antibiotic resistance profiles, making them suitable for further studies on a development of bacteriophage cocktail for potential therapeutic applications.

No MeSH data available.