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Tea ( Camellia sinensis ) infusions ameliorate cancer in 4TI metastatic breast cancer model

View Article: PubMed Central - PubMed

ABSTRACT

Background: Tea (Camellia sinensis) infusions are widely consumed beverages with numerous health benefits. However, physiological and molecular responses mediating these activities are poorly understood.

Method: Three replicates of 4TI cancer cell suspension (2.0 × 105 cells/ml) were challenged in vitro with various concentrations of green, black and purple tea infusions to asseses their cytoxicity and associated differentially expressed genes in the cells. Inhibitory activity was tested by using serial dilutions of respective tea infusions in a 96 well ELISA plate.

Results: Green tea had the highest inhibition on 4TI cells proliferation at a concentration of IC50 = 13.12 μg/ml. Further analysis of the 4TI cancer cell line treated with tea using 454 pyrosequencing generated 425,696 reads with an input mean length of 286.54. Trimmed sequences were imported on a CLC genomic workbench v7.03 and annotated on a reference mouse genome (Mus musculus strain C57BL/6 J). Results revealed a differential expression of apoptosis related genes in the transcriptome. Casp8, Casp9, Casp3, Casp6, Casp8AP2, Aifm1, Aifm2 and Apopt1 genes were significantly upregulated indicating the process of apoptosis was initiated and executed.

Conclusion: These findings on caspases offer valuable information on the mechanism of tea as an anticancer agent and will contribute to further research in future novel treatments.

No MeSH data available.


Gel-like image of the cDNA library samples as run on an Agilent bioanalyzer high sensitivity DNA chip. The samples produced a smear that ranged from 0.2 kb to 7 kb as shown in the arrow
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Fig8: Gel-like image of the cDNA library samples as run on an Agilent bioanalyzer high sensitivity DNA chip. The samples produced a smear that ranged from 0.2 kb to 7 kb as shown in the arrow

Mentions: cDNA libraries were synthesized from the isolated mRNA and the quality of the cDNA synthesized assessed using a DNA chip. A peak which ranged between 600 and 1200 bp was produced (Fig. 7). A gel like image presentation of the same also produced a thick band at the same fragment length as Fig. 8 which was within the required range for good quality cDNA.Fig. 7


Tea ( Camellia sinensis ) infusions ameliorate cancer in 4TI metastatic breast cancer model
Gel-like image of the cDNA library samples as run on an Agilent bioanalyzer high sensitivity DNA chip. The samples produced a smear that ranged from 0.2 kb to 7 kb as shown in the arrow
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5383982&req=5

Fig8: Gel-like image of the cDNA library samples as run on an Agilent bioanalyzer high sensitivity DNA chip. The samples produced a smear that ranged from 0.2 kb to 7 kb as shown in the arrow
Mentions: cDNA libraries were synthesized from the isolated mRNA and the quality of the cDNA synthesized assessed using a DNA chip. A peak which ranged between 600 and 1200 bp was produced (Fig. 7). A gel like image presentation of the same also produced a thick band at the same fragment length as Fig. 8 which was within the required range for good quality cDNA.Fig. 7

View Article: PubMed Central - PubMed

ABSTRACT

Background: Tea (Camellia sinensis) infusions are widely consumed beverages with numerous health benefits. However, physiological and molecular responses mediating these activities are poorly understood.

Method: Three replicates of 4TI cancer cell suspension (2.0 × 105 cells/ml) were challenged in vitro with various concentrations of green, black and purple tea infusions to asseses their cytoxicity and associated differentially expressed genes in the cells. Inhibitory activity was tested by using serial dilutions of respective tea infusions in a 96 well ELISA plate.

Results: Green tea had the highest inhibition on 4TI cells proliferation at a concentration of IC50 = 13.12 μg/ml. Further analysis of the 4TI cancer cell line treated with tea using 454 pyrosequencing generated 425,696 reads with an input mean length of 286.54. Trimmed sequences were imported on a CLC genomic workbench v7.03 and annotated on a reference mouse genome (Mus musculus strain C57BL/6 J). Results revealed a differential expression of apoptosis related genes in the transcriptome. Casp8, Casp9, Casp3, Casp6, Casp8AP2, Aifm1, Aifm2 and Apopt1 genes were significantly upregulated indicating the process of apoptosis was initiated and executed.

Conclusion: These findings on caspases offer valuable information on the mechanism of tea as an anticancer agent and will contribute to further research in future novel treatments.

No MeSH data available.