Limits...
Tea ( Camellia sinensis ) infusions ameliorate cancer in 4TI metastatic breast cancer model

View Article: PubMed Central - PubMed

ABSTRACT

Background: Tea (Camellia sinensis) infusions are widely consumed beverages with numerous health benefits. However, physiological and molecular responses mediating these activities are poorly understood.

Method: Three replicates of 4TI cancer cell suspension (2.0 × 105 cells/ml) were challenged in vitro with various concentrations of green, black and purple tea infusions to asseses their cytoxicity and associated differentially expressed genes in the cells. Inhibitory activity was tested by using serial dilutions of respective tea infusions in a 96 well ELISA plate.

Results: Green tea had the highest inhibition on 4TI cells proliferation at a concentration of IC50 = 13.12 μg/ml. Further analysis of the 4TI cancer cell line treated with tea using 454 pyrosequencing generated 425,696 reads with an input mean length of 286.54. Trimmed sequences were imported on a CLC genomic workbench v7.03 and annotated on a reference mouse genome (Mus musculus strain C57BL/6 J). Results revealed a differential expression of apoptosis related genes in the transcriptome. Casp8, Casp9, Casp3, Casp6, Casp8AP2, Aifm1, Aifm2 and Apopt1 genes were significantly upregulated indicating the process of apoptosis was initiated and executed.

Conclusion: These findings on caspases offer valuable information on the mechanism of tea as an anticancer agent and will contribute to further research in future novel treatments.

No MeSH data available.


Related in: MedlinePlus

Sensitivity of 4TI cancer cells to green tea at IC50 of 13.12 μg/ml showing disruption of monolayer
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC5383982&req=5

Fig5: Sensitivity of 4TI cancer cells to green tea at IC50 of 13.12 μg/ml showing disruption of monolayer

Mentions: However, when the 4TI cells were treated with black, purple andgreen tea an effect on the cell viability and proliferation was observed on the monolayer formation. Results from this study revealed that tea had an effect on cell growth by disrupting the monolayer formation which was evidenced by a reduction in the cell to cell contact and this was the reason why there was an ultimate reduction in the spreading of the cells (Figs. 3, 4 and 5). This remarkable observation was a clear indication that tea inhibited cell viability and proliferation.Fig. 3


Tea ( Camellia sinensis ) infusions ameliorate cancer in 4TI metastatic breast cancer model
Sensitivity of 4TI cancer cells to green tea at IC50 of 13.12 μg/ml showing disruption of monolayer
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5383982&req=5

Fig5: Sensitivity of 4TI cancer cells to green tea at IC50 of 13.12 μg/ml showing disruption of monolayer
Mentions: However, when the 4TI cells were treated with black, purple andgreen tea an effect on the cell viability and proliferation was observed on the monolayer formation. Results from this study revealed that tea had an effect on cell growth by disrupting the monolayer formation which was evidenced by a reduction in the cell to cell contact and this was the reason why there was an ultimate reduction in the spreading of the cells (Figs. 3, 4 and 5). This remarkable observation was a clear indication that tea inhibited cell viability and proliferation.Fig. 3

View Article: PubMed Central - PubMed

ABSTRACT

Background: Tea (Camellia sinensis) infusions are widely consumed beverages with numerous health benefits. However, physiological and molecular responses mediating these activities are poorly understood.

Method: Three replicates of 4TI cancer cell suspension (2.0 × 105 cells/ml) were challenged in vitro with various concentrations of green, black and purple tea infusions to asseses their cytoxicity and associated differentially expressed genes in the cells. Inhibitory activity was tested by using serial dilutions of respective tea infusions in a 96 well ELISA plate.

Results: Green tea had the highest inhibition on 4TI cells proliferation at a concentration of IC50 = 13.12 μg/ml. Further analysis of the 4TI cancer cell line treated with tea using 454 pyrosequencing generated 425,696 reads with an input mean length of 286.54. Trimmed sequences were imported on a CLC genomic workbench v7.03 and annotated on a reference mouse genome (Mus musculus strain C57BL/6 J). Results revealed a differential expression of apoptosis related genes in the transcriptome. Casp8, Casp9, Casp3, Casp6, Casp8AP2, Aifm1, Aifm2 and Apopt1 genes were significantly upregulated indicating the process of apoptosis was initiated and executed.

Conclusion: These findings on caspases offer valuable information on the mechanism of tea as an anticancer agent and will contribute to further research in future novel treatments.

No MeSH data available.


Related in: MedlinePlus