Limits...
Enhanced efficacy with reduced toxicity of chemotherapy drug 5-fluorouracil by synergistic treatment with Abnormal Savda Munziq from Uyghur medicine

View Article: PubMed Central - PubMed

ABSTRACT

Background: Abnormal Savda Munziq (ASMq) is a traditional prescription in Uyghur Medicine, and its treatment of complex diseases such as tumors and asthma has been proven to be effective in Uyghur medical clinical practice. The efficacy-enhancing and toxicity-reducing properties of ASMq were studied on mice with transplanted cervical cancer (U27) tumors, which were treated with 5-fluorouracil (5-FU) in this work.

Methods: To investigate the synergistic effect of ASMq and 5-FU on U27 cells, inhibitory effects on cell proliferation were determined through a MTT assay. 48 Kunming mice which were randomly divided in to 6 groups: control group, model group, 5-FU group, 5-FU combine with ASMq low-dose group, 5-FU combine with ASMq medium-dose group, and 5-FU combine with ASMq high- dose group, the inhibition rate of the tumor, the viscera indexes, and the content of serum tumor necrosis factor-α (TNF-α), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. The expression levels of transforming growth factor-β1 (TGF-β1) and human papillomavirus type 16 E2 (HPV16 E2) protein were assessed by Western blot. Pathological changes in the liver were observed.

Result: The inhibition rates of tumors, the 5-FU + ASMq.H group(80.64%), 5-FU + ASMq.M group (90.67%), 5-FU + ASMq.L group (72.03%) and 5-FU group (66.89%), clearly indicated that the effects of tumor inhibition. The thymus index and spleen index were increased, and the serum concentration of TNF-α increased while ALT and AST concentrations were decreased, and TNF-α protein expression were increased while TGF-β1 and HPV16 E2 were decreased. ASMq might can improve livers central vein hyperemia and interstitial edema, and preserve the radial structure of the hepatic cords.

Conclusions: The results suggested that ASMq might reduce toxicity and enhance the efficacy of the chemotherapeutic drug 5-fluorouracil in the treatment of cervical carcinoma.

No MeSH data available.


Liver pathology examination
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC5383980&req=5

Fig5: Liver pathology examination

Mentions: The pathological changes in the liver from all experimental groups were analyzed by HE staining. From the histological sections in Fig. 5. In the control group, the liver tissue structure was normal, and no pathological changes were found under low power lens or high power lens. In the model group, the liver tissue structure was not distinguished under low power lens, and the liver cells generally swelling to be granular or eosinophilic change. According to high power lens, the liver structure was not distinguishable, and the liver cells generally swollen and changed into granules. In the 5-FU group, the liver tissue structure was not distinguishable, and the cells were generally swollen and changed into granules, or had diffuse eosinophilic change, and the central vein was dilated. Under high power lens, the structure of liver cells was not distinguishable, and the cells were generally swollen and changed into granules, or having diffuse eosinophilic change. In the 5-FU + ASMq.H group, under low power lens, the structure of liver cells was not distinguished, and the cells were generally swollen and changed into granules, or having diffuse eosinophilic change. Under the high power lens, the structure of liver cells was not distinguishable, and the cells were generally swollen and changed into granules. In the 5-FU + ASMq.M group, under low power lens, the liver tissue structure was normal, and the liver cells showed slightly swollen and changed into granules, or diffuse eosinophilic change. Under high power lens, swelling of the liver cells showed granular degeneration or scattered eosinophilic degeneration, and liver sinus were not distinguished. In the 5-FU + ASMq.L group, under the low power lens, the liver tissue structure was not distinguished, and the cells generally swelled and became granular or scattered eosinophilic change, and focal necrosis were found. Under the low power microscope, the liver structure is not clear, cell swelling or granular scattered eosinophilic degeneration, and liver sinus were not distinguishable.Fig. 5


Enhanced efficacy with reduced toxicity of chemotherapy drug 5-fluorouracil by synergistic treatment with Abnormal Savda Munziq from Uyghur medicine
Liver pathology examination
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5383980&req=5

Fig5: Liver pathology examination
Mentions: The pathological changes in the liver from all experimental groups were analyzed by HE staining. From the histological sections in Fig. 5. In the control group, the liver tissue structure was normal, and no pathological changes were found under low power lens or high power lens. In the model group, the liver tissue structure was not distinguished under low power lens, and the liver cells generally swelling to be granular or eosinophilic change. According to high power lens, the liver structure was not distinguishable, and the liver cells generally swollen and changed into granules. In the 5-FU group, the liver tissue structure was not distinguishable, and the cells were generally swollen and changed into granules, or had diffuse eosinophilic change, and the central vein was dilated. Under high power lens, the structure of liver cells was not distinguishable, and the cells were generally swollen and changed into granules, or having diffuse eosinophilic change. In the 5-FU + ASMq.H group, under low power lens, the structure of liver cells was not distinguished, and the cells were generally swollen and changed into granules, or having diffuse eosinophilic change. Under the high power lens, the structure of liver cells was not distinguishable, and the cells were generally swollen and changed into granules. In the 5-FU + ASMq.M group, under low power lens, the liver tissue structure was normal, and the liver cells showed slightly swollen and changed into granules, or diffuse eosinophilic change. Under high power lens, swelling of the liver cells showed granular degeneration or scattered eosinophilic degeneration, and liver sinus were not distinguished. In the 5-FU + ASMq.L group, under the low power lens, the liver tissue structure was not distinguished, and the cells generally swelled and became granular or scattered eosinophilic change, and focal necrosis were found. Under the low power microscope, the liver structure is not clear, cell swelling or granular scattered eosinophilic degeneration, and liver sinus were not distinguishable.Fig. 5

View Article: PubMed Central - PubMed

ABSTRACT

Background: Abnormal Savda Munziq (ASMq) is a traditional prescription in Uyghur Medicine, and its treatment of complex diseases such as tumors and asthma has been proven to be effective in Uyghur medical clinical practice. The efficacy-enhancing and toxicity-reducing properties of ASMq were studied on mice with transplanted cervical cancer (U27) tumors, which were treated with 5-fluorouracil (5-FU) in this work.

Methods: To investigate the synergistic effect of ASMq and 5-FU on U27 cells, inhibitory effects on cell proliferation were determined through a MTT assay. 48 Kunming mice which were randomly divided in to 6 groups: control group, model group, 5-FU group, 5-FU combine with ASMq low-dose group, 5-FU combine with ASMq medium-dose group, and 5-FU combine with ASMq high- dose group, the inhibition rate of the tumor, the viscera indexes, and the content of serum tumor necrosis factor-α (TNF-α), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. The expression levels of transforming growth factor-β1 (TGF-β1) and human papillomavirus type 16 E2 (HPV16 E2) protein were assessed by Western blot. Pathological changes in the liver were observed.

Result: The inhibition rates of tumors, the 5-FU + ASMq.H group(80.64%), 5-FU + ASMq.M group (90.67%), 5-FU + ASMq.L group (72.03%) and 5-FU group (66.89%), clearly indicated that the effects of tumor inhibition. The thymus index and spleen index were increased, and the serum concentration of TNF-α increased while ALT and AST concentrations were decreased, and TNF-α protein expression were increased while TGF-β1 and HPV16 E2 were decreased. ASMq might can improve livers central vein hyperemia and interstitial edema, and preserve the radial structure of the hepatic cords.

Conclusions: The results suggested that ASMq might reduce toxicity and enhance the efficacy of the chemotherapeutic drug 5-fluorouracil in the treatment of cervical carcinoma.

No MeSH data available.