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Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study

View Article: PubMed Central - PubMed

ABSTRACT

Background: Nonalcoholic fatty liver disease (NAFLD) represents one of the most common forms of liver disease worldwide, and it is always regarded as a consequence of a sedentary, food-abundant lifestyle, sitting for an extended time, and a low physical activity level, which often coincide with chronic and long-lasting psychological stress. A Chinese medicine Sinisan (SNS) may be a potential formula for treating this kind of disease.

Methods: In this study, a long-term chronic restraint stress protocol was used to investigate the mechanism underlying stress-induced NALFD. To investigate the effect of SNS treatment on stress-induced NAFLD, we measured the liver and serum values of total cholesterol (TC), triglyceride (TG), liver free fatty acids (FFA), low-density lipoprotein, superoxide dismutase, tumor necrosis factor-α, malondialdehyde, interleukin (IL)-6, and serum values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase. Results are shown as a mean ± standard deviation. Significant differences between the groups were evaluated using the Student t-test. For multiple comparisons, one-way analysis of variance (ANOVA) was used. If the results of ANOVA indicated significant differences, post hoc analysis was performed with the Tukey test or Dunnett test, and p < 0.05 was considered statistically significant.

Results: Long-term chronic stress led to steatosis and non-alcoholic steatohepatitis. Additionally, SNS treatment significantly increased body weight gain (p < 0.01) and sucrose preference (p < 0.001), and it reduced the liver values of TC, TG, and FFA (p < 0.05). SNS also reduced the serum values of AST and ALT (p < 0.001), and the liver value of IL-6 (p < 0.01).

Conclusions: This study’s results demonstrate that psychological stress may be a significant risk factor of NAFLD. Furthermore, the traditional Chinese medicine formula SNS may have some beneficial effect in antagonizing psychological stress and stress-related NAFLD.

No MeSH data available.


Related in: MedlinePlus

The oxidative stress and inflammation of the liver. a The superoxide dismutase (SOD) level of each group. b The tumor necrosis factor (TNF)-α level of each group. c The malondialdehyde (MDA) level of each group. d The liver interleukin (IL)-6 level of each group. Values plotted are a mean ± standard deviation (N = 10 per group). #p < 0.05, ##p < 0.01 versus the control group, **p < 0.01 versus the stress group. SNS, Sinisan
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Fig5: The oxidative stress and inflammation of the liver. a The superoxide dismutase (SOD) level of each group. b The tumor necrosis factor (TNF)-α level of each group. c The malondialdehyde (MDA) level of each group. d The liver interleukin (IL)-6 level of each group. Values plotted are a mean ± standard deviation (N = 10 per group). #p < 0.05, ##p < 0.01 versus the control group, **p < 0.01 versus the stress group. SNS, Sinisan

Mentions: Results showed that stressed rats developed exacerbated NASH compared to the control rats, as indicated by increased levels of serum AST (p < 0.001) and ALT (p < 0.001), and NAFLD activity inflammation scores (p < 0.01) (Fig. 4a, b). No significant difference was observed in the serum alkaline phosphatase (ALP) level and AST/ALT ratio between the stress and control groups (Fig. 4c, d). Regarding the SOD (p < 0.01), MDA (p < 0.05), and IL-6 (p < 0.01) levels, stressed rats had higher liver levels than control rats, and concerning the TNF-α level, no significant increase was observed in the stress group (Fig. 5a-d).Fig. 4


Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study
The oxidative stress and inflammation of the liver. a The superoxide dismutase (SOD) level of each group. b The tumor necrosis factor (TNF)-α level of each group. c The malondialdehyde (MDA) level of each group. d The liver interleukin (IL)-6 level of each group. Values plotted are a mean ± standard deviation (N = 10 per group). #p < 0.05, ##p < 0.01 versus the control group, **p < 0.01 versus the stress group. SNS, Sinisan
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5383977&req=5

Fig5: The oxidative stress and inflammation of the liver. a The superoxide dismutase (SOD) level of each group. b The tumor necrosis factor (TNF)-α level of each group. c The malondialdehyde (MDA) level of each group. d The liver interleukin (IL)-6 level of each group. Values plotted are a mean ± standard deviation (N = 10 per group). #p < 0.05, ##p < 0.01 versus the control group, **p < 0.01 versus the stress group. SNS, Sinisan
Mentions: Results showed that stressed rats developed exacerbated NASH compared to the control rats, as indicated by increased levels of serum AST (p < 0.001) and ALT (p < 0.001), and NAFLD activity inflammation scores (p < 0.01) (Fig. 4a, b). No significant difference was observed in the serum alkaline phosphatase (ALP) level and AST/ALT ratio between the stress and control groups (Fig. 4c, d). Regarding the SOD (p < 0.01), MDA (p < 0.05), and IL-6 (p < 0.01) levels, stressed rats had higher liver levels than control rats, and concerning the TNF-α level, no significant increase was observed in the stress group (Fig. 5a-d).Fig. 4

View Article: PubMed Central - PubMed

ABSTRACT

Background: Nonalcoholic fatty liver disease (NAFLD) represents one of the most common forms of liver disease worldwide, and it is always regarded as a consequence of a sedentary, food-abundant lifestyle, sitting for an extended time, and a low physical activity level, which often coincide with chronic and long-lasting psychological stress. A Chinese medicine Sinisan (SNS) may be a potential formula for treating this kind of disease.

Methods: In this study, a long-term chronic restraint stress protocol was used to investigate the mechanism underlying stress-induced NALFD. To investigate the effect of SNS treatment on stress-induced NAFLD, we measured the liver and serum values of total cholesterol (TC), triglyceride (TG), liver free fatty acids (FFA), low-density lipoprotein, superoxide dismutase, tumor necrosis factor-&alpha;, malondialdehyde, interleukin (IL)-6, and serum values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase. Results are shown as a mean&nbsp;&plusmn;&nbsp;standard deviation. Significant differences between the groups were evaluated using the Student t-test. For multiple comparisons, one-way analysis of variance (ANOVA) was used. If the results of ANOVA indicated significant differences, post hoc analysis was performed with the Tukey test or Dunnett test, and p&nbsp;&lt;&nbsp;0.05 was considered statistically significant.

Results: Long-term chronic stress led to steatosis and non-alcoholic steatohepatitis. Additionally, SNS treatment significantly increased body weight gain (p&nbsp;&lt;&nbsp;0.01) and sucrose preference (p&nbsp;&lt;&nbsp;0.001), and it reduced the liver values of TC, TG, and FFA (p&nbsp;&lt;&nbsp;0.05). SNS also reduced the serum values of AST and ALT (p&nbsp;&lt;&nbsp;0.001), and the liver value of IL-6 (p&nbsp;&lt;&nbsp;0.01).

Conclusions: This study&rsquo;s results demonstrate that psychological stress may be a significant risk factor of NAFLD. Furthermore, the traditional Chinese medicine formula SNS may have some beneficial effect in antagonizing psychological stress and stress-related NAFLD.

No MeSH data available.


Related in: MedlinePlus