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Cytokinin, auxin, and abscisic acid affects sucrose metabolism conduce to de novo shoot organogenesis in rice ( Oryza sativa L.) callus

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ABSTRACT

Background: Shoot regeneration frequency in rice callus is still low and highly diverse among rice cultivars. This study aimed to investigate the association of plant hormone signaling and sucrose uptake and metabolism in rice during callus induction and early shoot organogenesis. The immatured seeds of two rice cultivars, Ai-Nan-Tsao 39 (ANT39) and Tainan 11 (TN11) are used in this study.

Results: Callus formation is earlier, callus fresh weight is higher, but water content is significant lower in ANT39 than in TN11 while their explants are inoculated on callus induction medium (CIM). Besides, the regeneration frequency is prominently higher in ANT39 (~80%) compared to TN11 callus (0%). Levels of glucose, sucrose, and starch are all significant higher in ANT39 than in TN11 either at callus induction or early shoot organogenesis stage. Moreover, high expression levels of Cell wall-bound invertase 1, Sucrose transporter 1 (OsSUT1) and OsSUT2 are detected in ANT39 at the fourth-day in CIM but it cannot be detected in TN11 until the tenth-day. It suggested that ANT39 has higher callus growth rate and shoot regeneration ability may cause from higher activity of sucrose uptake and metabolism. As well, the expression levels of ORYZA SATIVA RESPONSE REGULATOR 1 (ORR1), PIN-formed 1 and Late embryogenesis-abundant 1, representing endogenous cytokinin, auxin and ABA signals, respectively, were also up-regulated in highly regenerable callus, ANT39, but only ORR1 was greatly enhanced in TN11 at the tenth-day in CIM.

Conclusion: Thus, phytohormone signals may affect sucrose metabolism to trigger callus initiation and further de novo shoot regeneration in rice culture.

Electronic supplementary material: The online version of this article (doi:10.1186/1999-3110-54-5) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus

Carbohydrate content during callus induction and early shoot organogenesis in rice. Glucose, sucrose and starch content in ANT39 and TN11 calli inoculated in callus induction media (CIM; a-c) and regeneration media (RM; d-f). Data are mean ± standard error (n=3).
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Fig3: Carbohydrate content during callus induction and early shoot organogenesis in rice. Glucose, sucrose and starch content in ANT39 and TN11 calli inoculated in callus induction media (CIM; a-c) and regeneration media (RM; d-f). Data are mean ± standard error (n=3).

Mentions: To clarify the relationship between shoot organogenesis ability and carbohydrate metabolism, glucose, sucrose and starch contents were determined at callus induction and early shoot regeneration stage. The result showed that glucose, sucrose, and starch contents are all significant higher in the HR calli, ANT39, than in NR calli, TN11, either at callus induction or regeneration period (Figure 3). The high carbohydrate content in ANT39 was maintained during callus induction (Figure 3a-c), but the levels of glucose and starch are gradually decreased after transferred to RM in 7 days (Figure 3d, f). All glucose, sucrose, and starch contents in TN11 calli are low and have no significant change during the whole evaluation time. The carbohydrate utilization is higher in ANT39 than in TN11 calli would supply to the energy and osmotic requirement of callus formation and starch accumulation. ANT39 callus possesses high level of starch mainly caused from higher biosynthetic activity and is also observed in our previous study by histochemical analysis (Huang et al., 2006). Besides, high levels of cellular carbohydrates associated with shoot organogenesis in rice callus are similar to the regeneration system induced by osmotic stress (Huang and Liu, 2002).Figure 3


Cytokinin, auxin, and abscisic acid affects sucrose metabolism conduce to de novo shoot organogenesis in rice ( Oryza sativa L.) callus
Carbohydrate content during callus induction and early shoot organogenesis in rice. Glucose, sucrose and starch content in ANT39 and TN11 calli inoculated in callus induction media (CIM; a-c) and regeneration media (RM; d-f). Data are mean ± standard error (n=3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383921&req=5

Fig3: Carbohydrate content during callus induction and early shoot organogenesis in rice. Glucose, sucrose and starch content in ANT39 and TN11 calli inoculated in callus induction media (CIM; a-c) and regeneration media (RM; d-f). Data are mean ± standard error (n=3).
Mentions: To clarify the relationship between shoot organogenesis ability and carbohydrate metabolism, glucose, sucrose and starch contents were determined at callus induction and early shoot regeneration stage. The result showed that glucose, sucrose, and starch contents are all significant higher in the HR calli, ANT39, than in NR calli, TN11, either at callus induction or regeneration period (Figure 3). The high carbohydrate content in ANT39 was maintained during callus induction (Figure 3a-c), but the levels of glucose and starch are gradually decreased after transferred to RM in 7 days (Figure 3d, f). All glucose, sucrose, and starch contents in TN11 calli are low and have no significant change during the whole evaluation time. The carbohydrate utilization is higher in ANT39 than in TN11 calli would supply to the energy and osmotic requirement of callus formation and starch accumulation. ANT39 callus possesses high level of starch mainly caused from higher biosynthetic activity and is also observed in our previous study by histochemical analysis (Huang et al., 2006). Besides, high levels of cellular carbohydrates associated with shoot organogenesis in rice callus are similar to the regeneration system induced by osmotic stress (Huang and Liu, 2002).Figure 3

View Article: PubMed Central

ABSTRACT

Background: Shoot regeneration frequency in rice callus is still low and highly diverse among rice cultivars. This study aimed to investigate the association of plant hormone signaling and sucrose uptake and metabolism in rice during callus induction and early shoot organogenesis. The immatured seeds of two rice cultivars, Ai-Nan-Tsao 39 (ANT39) and Tainan 11 (TN11) are used in this study.

Results: Callus formation is earlier, callus fresh weight is higher, but water content is significant lower in ANT39 than in TN11 while their explants are inoculated on callus induction medium (CIM). Besides, the regeneration frequency is prominently higher in ANT39 (~80%) compared to TN11 callus (0%). Levels of glucose, sucrose, and starch are all significant higher in ANT39 than in TN11 either at callus induction or early shoot organogenesis stage. Moreover, high expression levels of Cell wall-bound invertase 1, Sucrose transporter 1 (OsSUT1) and OsSUT2 are detected in ANT39 at the fourth-day in CIM but it cannot be detected in TN11 until the tenth-day. It suggested that ANT39 has higher callus growth rate and shoot regeneration ability may cause from higher activity of sucrose uptake and metabolism. As well, the expression levels of ORYZA SATIVA RESPONSE REGULATOR 1 (ORR1), PIN-formed 1 and Late embryogenesis-abundant 1, representing endogenous cytokinin, auxin and ABA signals, respectively, were also up-regulated in highly regenerable callus, ANT39, but only ORR1 was greatly enhanced in TN11 at the tenth-day in CIM.

Conclusion: Thus, phytohormone signals may affect sucrose metabolism to trigger callus initiation and further de novo shoot regeneration in rice culture.

Electronic supplementary material: The online version of this article (doi:10.1186/1999-3110-54-5) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus