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A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

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ABSTRACT

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

No MeSH data available.


Reproducibility of biosensor responses to 0.5 mM (1) and 1 mM (2) glutamate during one working day (a) and operational stability of the biosensor responses to 1 mM glutamate during four days (b). Measurements in 25 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode
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Fig9: Reproducibility of biosensor responses to 0.5 mM (1) and 1 mM (2) glutamate during one working day (a) and operational stability of the biosensor responses to 1 mM glutamate during four days (b). Measurements in 25 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode

Mentions: The signal reproducibility and operational stability are the key characteristics of any biosensor. To determine reproducibility of signals of the GlOx/silicalite biosensor, the responses to glutamate of two concentrations (0.5 and 1 mM) were measured over one working day with 20-min intervals; between measurements, the biosensor was kept in the buffer with continuous stirring (Fig. 9a). Relative standard deviations were 5.7 and 6.9%, respectively.Fig. 9


A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite
Reproducibility of biosensor responses to 0.5 mM (1) and 1 mM (2) glutamate during one working day (a) and operational stability of the biosensor responses to 1 mM glutamate during four days (b). Measurements in 25 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383914&req=5

Fig9: Reproducibility of biosensor responses to 0.5 mM (1) and 1 mM (2) glutamate during one working day (a) and operational stability of the biosensor responses to 1 mM glutamate during four days (b). Measurements in 25 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode
Mentions: The signal reproducibility and operational stability are the key characteristics of any biosensor. To determine reproducibility of signals of the GlOx/silicalite biosensor, the responses to glutamate of two concentrations (0.5 and 1 mM) were measured over one working day with 20-min intervals; between measurements, the biosensor was kept in the buffer with continuous stirring (Fig. 9a). Relative standard deviations were 5.7 and 6.9%, respectively.Fig. 9

View Article: PubMed Central - PubMed

ABSTRACT

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

No MeSH data available.