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A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

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ABSTRACT

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

No MeSH data available.


Typical responses of biosensor, based on GlOx adsorbed on silicalite, to 20 μM glutamate. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode
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Fig7: Typical responses of biosensor, based on GlOx adsorbed on silicalite, to 20 μM glutamate. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode

Mentions: After optimization of the procedure of coating of the amperometric sensor with silicalite layer and GlOx adsorption on silicalite, it was interesting to check main analytical characteristics of the obtained biosensor for glutamate determination. To demonstrate the biosensor work under optimal conditions, typical responses of the biosensor, to 20 μM glutamate is shown in Fig. 7. As seen, the measured responses were quite pronounced with a slight noise of baseline. In our view, the fluctuations of signal level after adding glutamate are because of either the noise caused by stirrer or the variations of oxygen level in the membrane. The minimum response time was 20–30 s.Fig. 7


A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite
Typical responses of biosensor, based on GlOx adsorbed on silicalite, to 20 μM glutamate. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5383914&req=5

Fig7: Typical responses of biosensor, based on GlOx adsorbed on silicalite, to 20 μM glutamate. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode
Mentions: After optimization of the procedure of coating of the amperometric sensor with silicalite layer and GlOx adsorption on silicalite, it was interesting to check main analytical characteristics of the obtained biosensor for glutamate determination. To demonstrate the biosensor work under optimal conditions, typical responses of the biosensor, to 20 μM glutamate is shown in Fig. 7. As seen, the measured responses were quite pronounced with a slight noise of baseline. In our view, the fluctuations of signal level after adding glutamate are because of either the noise caused by stirrer or the variations of oxygen level in the membrane. The minimum response time was 20–30 s.Fig. 7

View Article: PubMed Central - PubMed

ABSTRACT

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

No MeSH data available.