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A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

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ABSTRACT

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

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Dependence of responses of biosensor based on GlOx, adsorbed on silicalite, on concentration of silicalite deposited on transducer during 90 s. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode. Glutamate concentration, 1 mM
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Fig5: Dependence of responses of biosensor based on GlOx, adsorbed on silicalite, on concentration of silicalite deposited on transducer during 90 s. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode. Glutamate concentration, 1 mM

Mentions: Next, it was necessary to determine the optimal concentration of silicalite when forming its layer on the surface of platinum disc electrode of GlOx-based biosensor (Fig. 5). Deposition of 2.5% silicalite significantly increased the bioselective element activity compared with 0.25% silicalite, but higher silicalite concentration had no notable effect. Therefore, in further studies, GlOx was adsorbed on the amperometric transducers covered with 2.5% silicalite suspension.Fig. 5


A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite
Dependence of responses of biosensor based on GlOx, adsorbed on silicalite, on concentration of silicalite deposited on transducer during 90 s. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode. Glutamate concentration, 1 mM
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383914&req=5

Fig5: Dependence of responses of biosensor based on GlOx, adsorbed on silicalite, on concentration of silicalite deposited on transducer during 90 s. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode. Glutamate concentration, 1 mM
Mentions: Next, it was necessary to determine the optimal concentration of silicalite when forming its layer on the surface of platinum disc electrode of GlOx-based biosensor (Fig. 5). Deposition of 2.5% silicalite significantly increased the bioselective element activity compared with 0.25% silicalite, but higher silicalite concentration had no notable effect. Therefore, in further studies, GlOx was adsorbed on the amperometric transducers covered with 2.5% silicalite suspension.Fig. 5

View Article: PubMed Central - PubMed

ABSTRACT

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

No MeSH data available.


Related in: MedlinePlus