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A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

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ABSTRACT

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

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Dependence of responses of biosensor based on GlOx, adsorbed on silicalite, on the time of silicalite deposition on transducer. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode. Glutamate concentration, 1 mM
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Fig4: Dependence of responses of biosensor based on GlOx, adsorbed on silicalite, on the time of silicalite deposition on transducer. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode. Glutamate concentration, 1 mM

Mentions: First, the procedure of deposition of 5% of silicalite on the transducer was carried out with different time duration of heating at 150 °C from 10 to 960 s in the oven. It allowed a controllable increase in amount of silicalite on transducer and made it possible to explore an effect of this option on the final biosensor sensitivity to glutamate (1 mM). The dependence of biosensor responses on the time of silicalite heating on the electrode surface is shown in Fig. 4. As seen, the time of silicalite layer formation on the platinum electrode surface should be at least 60–90 s.Fig. 4


A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite
Dependence of responses of biosensor based on GlOx, adsorbed on silicalite, on the time of silicalite deposition on transducer. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode. Glutamate concentration, 1 mM
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383914&req=5

Fig4: Dependence of responses of biosensor based on GlOx, adsorbed on silicalite, on the time of silicalite deposition on transducer. Measurements in 20 mM HEPES buffer, pH 7.4, at a constant potential of +0.6 V vs Ag/AgCl reference electrode. Glutamate concentration, 1 mM
Mentions: First, the procedure of deposition of 5% of silicalite on the transducer was carried out with different time duration of heating at 150 °C from 10 to 960 s in the oven. It allowed a controllable increase in amount of silicalite on transducer and made it possible to explore an effect of this option on the final biosensor sensitivity to glutamate (1 mM). The dependence of biosensor responses on the time of silicalite heating on the electrode surface is shown in Fig. 4. As seen, the time of silicalite layer formation on the platinum electrode surface should be at least 60–90 s.Fig. 4

View Article: PubMed Central - PubMed

ABSTRACT

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

No MeSH data available.


Related in: MedlinePlus