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Pulse of inflammatory proteins in the pregnant uterus of European polecats ( Mustela putorius ) leading to the time of implantation

View Article: PubMed Central - PubMed

ABSTRACT

Uterine secretory proteins protect the uterus and conceptuses against infection, facilitate implantation, control cellular damage resulting from implantation, and supply pre-implantation embryos with nutrients. Unlike in humans, the early conceptus of the European polecat (Mustela putorius; ferret) grows and develops free in the uterus until implanting at about 12 days after mating. We found that the proteins appearing in polecat uteri changed dramatically with time leading to implantation. Several of these proteins have also been found in pregnant uteri of other eutherian mammals. However, we found a combination of two increasingly abundant proteins that have not been recorded before in pre-placentation uteri. First, the broad-spectrum proteinase inhibitor α2-macroglobulin rose to dominate the protein profile by the time of implantation. Its functions may be to limit damage caused by the release of proteinases during implantation or infection, and to control other processes around sites of implantation. Second, lipocalin-1 (also known as tear lipocalin) also increased substantially in concentration. This protein has not previously been recorded as a uterine secretion in pregnancy in any species. If polecat lipocalin-1 has similar biological properties to that of humans, then it may have a combined function in antimicrobial protection and transporting or scavenging lipids. The changes in the uterine secretory protein repertoire of European polecats is therefore unusual, and may be representative of pre-placentation supportive uterine secretions in mustelids (otters, weasels, badgers, mink, wolverines) in general.

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Subunit composition of major proteins in European polecat uterine flush following protein reduction. The labelled bands were excised from a non-reducing preparative SDS-PAGE gel of a uterine flush sample taken from a pregnant animal on day 12 after mating and re-run under reducing conditions. The letter codes for each band are consistent with those in figure 1. The two proteins that most notably increased in concentration with time were α2-macroglobulin (bands A and B) and lipocalin-1 (band G). The original protein samples were run under reducing (R) or non-reducing (NR) conditions. M, marker/calibration proteins with relative mobilities (Mr) indicated in kilodaltons (kDa).
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RSOS161085F2: Subunit composition of major proteins in European polecat uterine flush following protein reduction. The labelled bands were excised from a non-reducing preparative SDS-PAGE gel of a uterine flush sample taken from a pregnant animal on day 12 after mating and re-run under reducing conditions. The letter codes for each band are consistent with those in figure 1. The two proteins that most notably increased in concentration with time were α2-macroglobulin (bands A and B) and lipocalin-1 (band G). The original protein samples were run under reducing (R) or non-reducing (NR) conditions. M, marker/calibration proteins with relative mobilities (Mr) indicated in kilodaltons (kDa).

Mentions: Identification of the proteins isolated from bands excised from the protein electrophoresis gels indicated in figures 1 and 2.


Pulse of inflammatory proteins in the pregnant uterus of European polecats ( Mustela putorius ) leading to the time of implantation
Subunit composition of major proteins in European polecat uterine flush following protein reduction. The labelled bands were excised from a non-reducing preparative SDS-PAGE gel of a uterine flush sample taken from a pregnant animal on day 12 after mating and re-run under reducing conditions. The letter codes for each band are consistent with those in figure 1. The two proteins that most notably increased in concentration with time were α2-macroglobulin (bands A and B) and lipocalin-1 (band G). The original protein samples were run under reducing (R) or non-reducing (NR) conditions. M, marker/calibration proteins with relative mobilities (Mr) indicated in kilodaltons (kDa).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383852&req=5

RSOS161085F2: Subunit composition of major proteins in European polecat uterine flush following protein reduction. The labelled bands were excised from a non-reducing preparative SDS-PAGE gel of a uterine flush sample taken from a pregnant animal on day 12 after mating and re-run under reducing conditions. The letter codes for each band are consistent with those in figure 1. The two proteins that most notably increased in concentration with time were α2-macroglobulin (bands A and B) and lipocalin-1 (band G). The original protein samples were run under reducing (R) or non-reducing (NR) conditions. M, marker/calibration proteins with relative mobilities (Mr) indicated in kilodaltons (kDa).
Mentions: Identification of the proteins isolated from bands excised from the protein electrophoresis gels indicated in figures 1 and 2.

View Article: PubMed Central - PubMed

ABSTRACT

Uterine secretory proteins protect the uterus and conceptuses against infection, facilitate implantation, control cellular damage resulting from implantation, and supply pre-implantation embryos with nutrients. Unlike in humans, the early conceptus of the European polecat (Mustela putorius; ferret) grows and develops free in the uterus until implanting at about 12 days after mating. We found that the proteins appearing in polecat uteri changed dramatically with time leading to implantation. Several of these proteins have also been found in pregnant uteri of other eutherian mammals. However, we found a combination of two increasingly abundant proteins that have not been recorded before in pre-placentation uteri. First, the broad-spectrum proteinase inhibitor α2-macroglobulin rose to dominate the protein profile by the time of implantation. Its functions may be to limit damage caused by the release of proteinases during implantation or infection, and to control other processes around sites of implantation. Second, lipocalin-1 (also known as tear lipocalin) also increased substantially in concentration. This protein has not previously been recorded as a uterine secretion in pregnancy in any species. If polecat lipocalin-1 has similar biological properties to that of humans, then it may have a combined function in antimicrobial protection and transporting or scavenging lipids. The changes in the uterine secretory protein repertoire of European polecats is therefore unusual, and may be representative of pre-placentation supportive uterine secretions in mustelids (otters, weasels, badgers, mink, wolverines) in general.

No MeSH data available.


Related in: MedlinePlus