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Concurrence in the ability for lipid synthesis between life stages in insects

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ABSTRACT

The ability to synthesize lipids is critical for an organism’s fitness; hence, metabolic pathways, underlying lipid synthesis, tend to be highly conserved. Surprisingly, the majority of parasitoids deviate from this general metabolic model by lacking the ability to convert sugars and other carbohydrates into lipids. These insects spend the first part of their life feeding and developing in or on an arthropod host, during which they can carry over a substantial amount of lipid reserves. While many parasitoid species have been tested for lipogenic ability at the adult life stage, it has remained unclear whether parasitoid larvae can synthesize lipids. Here we investigate whether or not several insects can synthesize lipids during the larval stage using three ectoparasitic wasps (developing on the outside of the host) and the vinegar fly Drosophila melanogaster that differ in lipogenic ability in the adult life stage. Using feeding experiments and stable isotope tracing with gas chromatography/mass spectrometry, we first confirm lipogenic abilities in the adult life stage. Using topical application of stable isotopes in developing larvae, we then provide clear evidence of concurrence in lipogenic ability between larval and adult life stages in all species tested.

No MeSH data available.


Deuterium incorporation for larvae. Asterisks indicate significant differences in incorporation between larvae receiving the deuterium treatment and those receiving water controls. Points and error bars denote log-transformed adjusted mean per cent incorporation and 95% CIs, respectively.
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RSOS160815F2: Deuterium incorporation for larvae. Asterisks indicate significant differences in incorporation between larvae receiving the deuterium treatment and those receiving water controls. Points and error bars denote log-transformed adjusted mean per cent incorporation and 95% CIs, respectively.

Mentions: Isotope tracing following topical application of a water or deuterium solution was performed for larvae of all species. Linear mixed-effects models of larval incorporation showed significant main effects of species and treatment, but no effect of chain length (table 1). Interactions between factors were also significant (table 1). Incorporation was absent in E. vuilleti larvae (t30.0=0.095, p=0.925), whereas larvae of D. melanogaster (t19.96=2.11, p=0.0475), G. agilis (t171.56=4.936, p<0.0001) and G. areator (t228.52=4.495, p<0.0001) readily incorporated deuterium in their FA fractions (figure 2) when compared with water-only treatments.Figure 2.


Concurrence in the ability for lipid synthesis between life stages in insects
Deuterium incorporation for larvae. Asterisks indicate significant differences in incorporation between larvae receiving the deuterium treatment and those receiving water controls. Points and error bars denote log-transformed adjusted mean per cent incorporation and 95% CIs, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383825&req=5

RSOS160815F2: Deuterium incorporation for larvae. Asterisks indicate significant differences in incorporation between larvae receiving the deuterium treatment and those receiving water controls. Points and error bars denote log-transformed adjusted mean per cent incorporation and 95% CIs, respectively.
Mentions: Isotope tracing following topical application of a water or deuterium solution was performed for larvae of all species. Linear mixed-effects models of larval incorporation showed significant main effects of species and treatment, but no effect of chain length (table 1). Interactions between factors were also significant (table 1). Incorporation was absent in E. vuilleti larvae (t30.0=0.095, p=0.925), whereas larvae of D. melanogaster (t19.96=2.11, p=0.0475), G. agilis (t171.56=4.936, p<0.0001) and G. areator (t228.52=4.495, p<0.0001) readily incorporated deuterium in their FA fractions (figure 2) when compared with water-only treatments.Figure 2.

View Article: PubMed Central - PubMed

ABSTRACT

The ability to synthesize lipids is critical for an organism&rsquo;s fitness; hence, metabolic pathways, underlying lipid synthesis, tend to be highly conserved. Surprisingly, the majority of parasitoids deviate from this general metabolic model by lacking the ability to convert sugars and other carbohydrates into lipids. These insects spend the first part of their life feeding and developing in or on an arthropod host, during which they can carry over a substantial amount of lipid reserves. While many parasitoid species have been tested for lipogenic ability at the adult life stage, it has remained unclear whether parasitoid larvae can synthesize lipids. Here we investigate whether or not several insects can synthesize lipids during the larval stage using three ectoparasitic wasps (developing on the outside of the host) and the vinegar fly Drosophila melanogaster that differ in lipogenic ability in the adult life stage. Using feeding experiments and stable isotope tracing with gas chromatography/mass spectrometry, we first confirm lipogenic abilities in the adult life stage. Using topical application of stable isotopes in developing larvae, we then provide clear evidence of concurrence in lipogenic ability between larval and adult life stages in all species tested.

No MeSH data available.