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Improved efficacy of ultrafiltered xylanase – pectinase concoction in biobleaching of plywood waste soda pulp

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ABSTRACT

The effect of ultrafiltered xylanase–pectinase concoction produced simultaneously by a bacterial isolate using agro-waste-based media was assessed in prebleaching of plywood waste pulp. Ultrafiltered enzymes caused 12.5% reduction in kappa number at reduced enzyme dose of xylanase–pectinase (4.0–0.8 IU) per gram of pulp under optimized conditions at pH 8.5, temperature 55 °C, and treatment period of 2 h. Using this methodology, amount of Cl2–ClO2 consumption can be reduced up to 30 and 28.86%. Significant improvement in physical and optical properties of pulp was obtained along with an additional reduction in BOD and COD values up to 18.13 and 21.66% using this novel biodelignification approach. This is the first report showing the advantages of using ultrafiltered xylanase–pectinase over crude enzymes in enhancing the bleaching capacity of pulp. This study focussing on the development of good quality paper with less pollution generating strategy will definitely prove a boon for industries.

No MeSH data available.


Effect of enzyme dose, pH, treatment time, and temperature on pulp biobleaching. a These experiments were performed by treating the pulp at 55 °C for 180 min. For enzyme dose optimization, pH was set at 8.5, and for pH optimization, 4.0 IU of xylanase and 0.8 IU of pectinase dose per g of pulp were used. b These experiments were performed at pH 8.5 and enzyme dose of 4 IU of xylanase and 0.8 IU of pectinase per g of pulp. For treatment time optimization, reaction was allowed to proceed at 55 °C, and for temperature determination, treatment time was 120 min
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Fig1: Effect of enzyme dose, pH, treatment time, and temperature on pulp biobleaching. a These experiments were performed by treating the pulp at 55 °C for 180 min. For enzyme dose optimization, pH was set at 8.5, and for pH optimization, 4.0 IU of xylanase and 0.8 IU of pectinase dose per g of pulp were used. b These experiments were performed at pH 8.5 and enzyme dose of 4 IU of xylanase and 0.8 IU of pectinase per g of pulp. For treatment time optimization, reaction was allowed to proceed at 55 °C, and for temperature determination, treatment time was 120 min

Mentions: Maximum bleaching of plywood veneer SAQ pulp was observed with enzyme dose of 4.0 IU xylanase and 0.8 IU pectinase per g of pulp at pH 8.5 using UF enzymes (Fig. 1a), whereas 6.0 IU xylanase and 2.4 IU pectinase per g of pulp were found to be optimum for the bleaching of SAQ veneer pulp using crude enzymes at pH 8.5 (Agrawal et al. 2016). Ultrafiltration strategy resulted in the removal of low-molecular weight impurities from the crude xylanase–pectinase preparation, which was interfering with the kinetic efficiency of enzymes. Kaur et al. (2010) reported maximum prebleaching of mixed hardwood and bamboo kraft pulp with enzyme dose of 4.5 IU xylanase and 0.9 IU pectinase at pH 8.5. Xylanase dose of 10 IU/g of pulp at pH 9.0 was found to be optimum for biobleaching of mixed wood kraft pulp (Nagar et al. 2013).Fig. 1


Improved efficacy of ultrafiltered xylanase – pectinase concoction in biobleaching of plywood waste soda pulp
Effect of enzyme dose, pH, treatment time, and temperature on pulp biobleaching. a These experiments were performed by treating the pulp at 55 °C for 180 min. For enzyme dose optimization, pH was set at 8.5, and for pH optimization, 4.0 IU of xylanase and 0.8 IU of pectinase dose per g of pulp were used. b These experiments were performed at pH 8.5 and enzyme dose of 4 IU of xylanase and 0.8 IU of pectinase per g of pulp. For treatment time optimization, reaction was allowed to proceed at 55 °C, and for temperature determination, treatment time was 120 min
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5383790&req=5

Fig1: Effect of enzyme dose, pH, treatment time, and temperature on pulp biobleaching. a These experiments were performed by treating the pulp at 55 °C for 180 min. For enzyme dose optimization, pH was set at 8.5, and for pH optimization, 4.0 IU of xylanase and 0.8 IU of pectinase dose per g of pulp were used. b These experiments were performed at pH 8.5 and enzyme dose of 4 IU of xylanase and 0.8 IU of pectinase per g of pulp. For treatment time optimization, reaction was allowed to proceed at 55 °C, and for temperature determination, treatment time was 120 min
Mentions: Maximum bleaching of plywood veneer SAQ pulp was observed with enzyme dose of 4.0 IU xylanase and 0.8 IU pectinase per g of pulp at pH 8.5 using UF enzymes (Fig. 1a), whereas 6.0 IU xylanase and 2.4 IU pectinase per g of pulp were found to be optimum for the bleaching of SAQ veneer pulp using crude enzymes at pH 8.5 (Agrawal et al. 2016). Ultrafiltration strategy resulted in the removal of low-molecular weight impurities from the crude xylanase–pectinase preparation, which was interfering with the kinetic efficiency of enzymes. Kaur et al. (2010) reported maximum prebleaching of mixed hardwood and bamboo kraft pulp with enzyme dose of 4.5 IU xylanase and 0.9 IU pectinase at pH 8.5. Xylanase dose of 10 IU/g of pulp at pH 9.0 was found to be optimum for biobleaching of mixed wood kraft pulp (Nagar et al. 2013).Fig. 1

View Article: PubMed Central - PubMed

ABSTRACT

The effect of ultrafiltered xylanase–pectinase concoction produced simultaneously by a bacterial isolate using agro-waste-based media was assessed in prebleaching of plywood waste pulp. Ultrafiltered enzymes caused 12.5% reduction in kappa number at reduced enzyme dose of xylanase–pectinase (4.0–0.8 IU) per gram of pulp under optimized conditions at pH 8.5, temperature 55 °C, and treatment period of 2 h. Using this methodology, amount of Cl2–ClO2 consumption can be reduced up to 30 and 28.86%. Significant improvement in physical and optical properties of pulp was obtained along with an additional reduction in BOD and COD values up to 18.13 and 21.66% using this novel biodelignification approach. This is the first report showing the advantages of using ultrafiltered xylanase–pectinase over crude enzymes in enhancing the bleaching capacity of pulp. This study focussing on the development of good quality paper with less pollution generating strategy will definitely prove a boon for industries.

No MeSH data available.