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RAPD assisted selection of black gram ( Vigna mungo L. Hepper) towards the development of multiple disease resistant germplasm

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ABSTRACT

Black gram (Vigna mungo L. Hepper), is an extensively studied food crop which is affected by many abiotic and biotic factors, especially diseases. The yield potential of Black gram is shallow due to lack of genetic variability and biotic stress susceptibility. Core biotic stress factors include mung bean yellow mosaic virus (MYMV), urdbean leaf crinkle virus (UCLV), wilt (Fusarium oxysporum) and powdery mildew (Erysiphe polygoni DC). Although many studies determine resistant varieties to a particular disease, however, it is often complimented by low yield and susceptibility to other diseases. Hence, this study focuses on investigating the genetic relationships among three varieties and nine accessions of black gram having disease resistance to previously described diseases and susceptibility using random amplified polymorphic deoxyribonucleic acid (RAPD) markers. A total of 33 RAPD primers were used for diversity analysis and yielded 206 fragments. Number of amplified fragments ranged from two (OPN-1) to 13 (OPF-1). The highest similarity coefficient was observed between IC-145202 and IC-164118 (0.921), while lowest similarity was between PU-31 and IC-145202 (0.572). The genetic diversity obtained in this study along with disease analysis suggests PU31as a useful variety for the development of markers linked to MYMV, UCLV, wilt and powdery mildew resistance by marker-assisted back cross breeding and facilitates the production of crosses with multiple disease resistance.

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Dendogram of disease resistant and susceptible varieties, along with nine accessions using Jaccard’s similarity coefficient and UPGMA clustering. (MYMV mung bean yellow mosaic virus, UCLV urdbean leaf crinkle virus, PM powdery mildew)
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Fig3: Dendogram of disease resistant and susceptible varieties, along with nine accessions using Jaccard’s similarity coefficient and UPGMA clustering. (MYMV mung bean yellow mosaic virus, UCLV urdbean leaf crinkle virus, PM powdery mildew)

Mentions: After performing SAHN clustering on the binary matrix with subsequent calculation of Jaccard’s similarity dendrogram, the major cluster of 15 genotypes was divided into two sub-clusters, i.e., cluster I and cluster II at 64% of similarity. Cluster I accommodated PU-31, PU-205, PU-1075, LBG-623, IC-1575, IC-20880, LBG-645 and LBG-685, while cluster II comprised IC-59718, IC-61063, IC-61106, IC-73306, IC-110790, IC-145202 and IC-164118. Cluster I was further divided into two sub-clusters, i.e., Cluster Ia and Cluster Ib at 66% genetic similarity. Cluster Ia included three varieties, namely PU-31, PU-205 and PU-1075 displayed 74% similarity with remaining samples of cluster Ib. Cluster Ib was further divided into two clusters Ib1 and Ib2 at 73% of similarity. Cluster Ib1 had one MYMV susceptible variety (LBG-623) and two MYMV susceptible accessions, i.e., IC-1575 and IC-20880 and displayed 76% similarity with remaining samples of cluster Ib2. Ib2 accommodated two MYMV susceptible varieties, i.e., LBG-645 and LBG-685 which displayed 79% similarity with all the remaining samples in the cluster. Cluster II was further divided into two sub-clusters, i.e., IIa and IIb at 77% of similarity. The sub-cluster IIa contained one accession, i.e., IC-59718 which displayed 74% similarity with all remaining samples of cluster IIb and sub-cluster IIb was further divided into two clusters IIb1 and IIb2. IIb1 cluster displayed three accessions, i.e., IC-61063, IC-61106 and IC-73306 displayed 77% similarity with remaining samples of cluster IIb2 cluster. Cluster IIb2 showed three accessions IC-110790, IC-145202 and IC-164118 displayed 88% similarity with all the remaining samples in the cluster (Fig. 3).Fig. 3


RAPD assisted selection of black gram ( Vigna mungo L. Hepper) towards the development of multiple disease resistant germplasm
Dendogram of disease resistant and susceptible varieties, along with nine accessions using Jaccard’s similarity coefficient and UPGMA clustering. (MYMV mung bean yellow mosaic virus, UCLV urdbean leaf crinkle virus, PM powdery mildew)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383788&req=5

Fig3: Dendogram of disease resistant and susceptible varieties, along with nine accessions using Jaccard’s similarity coefficient and UPGMA clustering. (MYMV mung bean yellow mosaic virus, UCLV urdbean leaf crinkle virus, PM powdery mildew)
Mentions: After performing SAHN clustering on the binary matrix with subsequent calculation of Jaccard’s similarity dendrogram, the major cluster of 15 genotypes was divided into two sub-clusters, i.e., cluster I and cluster II at 64% of similarity. Cluster I accommodated PU-31, PU-205, PU-1075, LBG-623, IC-1575, IC-20880, LBG-645 and LBG-685, while cluster II comprised IC-59718, IC-61063, IC-61106, IC-73306, IC-110790, IC-145202 and IC-164118. Cluster I was further divided into two sub-clusters, i.e., Cluster Ia and Cluster Ib at 66% genetic similarity. Cluster Ia included three varieties, namely PU-31, PU-205 and PU-1075 displayed 74% similarity with remaining samples of cluster Ib. Cluster Ib was further divided into two clusters Ib1 and Ib2 at 73% of similarity. Cluster Ib1 had one MYMV susceptible variety (LBG-623) and two MYMV susceptible accessions, i.e., IC-1575 and IC-20880 and displayed 76% similarity with remaining samples of cluster Ib2. Ib2 accommodated two MYMV susceptible varieties, i.e., LBG-645 and LBG-685 which displayed 79% similarity with all the remaining samples in the cluster. Cluster II was further divided into two sub-clusters, i.e., IIa and IIb at 77% of similarity. The sub-cluster IIa contained one accession, i.e., IC-59718 which displayed 74% similarity with all remaining samples of cluster IIb and sub-cluster IIb was further divided into two clusters IIb1 and IIb2. IIb1 cluster displayed three accessions, i.e., IC-61063, IC-61106 and IC-73306 displayed 77% similarity with remaining samples of cluster IIb2 cluster. Cluster IIb2 showed three accessions IC-110790, IC-145202 and IC-164118 displayed 88% similarity with all the remaining samples in the cluster (Fig. 3).Fig. 3

View Article: PubMed Central - PubMed

ABSTRACT

Black gram (Vigna mungo L. Hepper), is an extensively studied food crop which is affected by many abiotic and biotic factors, especially diseases. The yield potential of Black gram is shallow due to lack of genetic variability and biotic stress susceptibility. Core biotic stress factors include mung bean yellow mosaic virus (MYMV), urdbean leaf crinkle virus (UCLV), wilt (Fusarium oxysporum) and powdery mildew (Erysiphe polygoni DC). Although many studies determine resistant varieties to a particular disease, however, it is often complimented by low yield and susceptibility to other diseases. Hence, this study focuses on investigating the genetic relationships among three varieties and nine accessions of black gram having disease resistance to previously described diseases and susceptibility using random amplified polymorphic deoxyribonucleic acid (RAPD) markers. A total of 33 RAPD primers were used for diversity analysis and yielded 206 fragments. Number of amplified fragments ranged from two (OPN-1) to 13 (OPF-1). The highest similarity coefficient was observed between IC-145202 and IC-164118 (0.921), while lowest similarity was between PU-31 and IC-145202 (0.572). The genetic diversity obtained in this study along with disease analysis suggests PU31as a useful variety for the development of markers linked to MYMV, UCLV, wilt and powdery mildew resistance by marker-assisted back cross breeding and facilitates the production of crosses with multiple disease resistance.

No MeSH data available.


Related in: MedlinePlus