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Establishment of heterotopic ossification via sharp instrument injury in rats

View Article: PubMed Central - PubMed

ABSTRACT

Objective:: To establish an animal model for heterotopic ossification (HO) induced by sharp instrument injury in Sprague-Dawley (SD) rat and to investigate its possible mechanism.

Materials and methods:: A total of 48 male SD rats were divided into 3 groups (n=16). In sham group, incision and suture were performed only in the left leg. Partial tenotomy was performed in the left Achilles tendons in the PAT group. In Achilles’ tenotomy (AT) group, tenotomy was performed in the left Achilles tendons to establish animal model of EO. X-ray and histological examinations were made at 6 and 10 weeks after operation.

Results:: No HO occurred in the sham and PAT groups. In AT group, X–ray results on 4 rats showed cartilage and bone formation while the remaining 4 rats showed chondrification in histological examination at 6 weeks after operation. At 10 weeks all rats showed bone formation with trabecular bone. This kind of HO usually develops through a process of endochondral ossification.

Conclusion:: Tenotomy is a simple, effective and feasible method to induce HO.

No MeSH data available.


Related in: MedlinePlus

Histological examination results: (A) Sham group at the 10th week after operation, normal Achilles tendon tissues (HE, 400X); (B) PAT group at the 10th week after operation, degenerated Achilles tendon tissues (HE, 400X); (C) AT group at the 6th week after operation, cartilage cells seen inside degenerated Achilles tendon (HE, 400X); (D) AT group at the 10th week after operation, mature bone tissues, trabecular structure and marrow cavity could be seen (HE, 400X); (E) AT group at the 6th week after operation, bone lacuna and trabecular bone structure could be seen (Masson, 400X); (F) AT group at the 10th week after operation, mature lamellar bone structure could be seen (Masson, 400X).
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Figure 2: Histological examination results: (A) Sham group at the 10th week after operation, normal Achilles tendon tissues (HE, 400X); (B) PAT group at the 10th week after operation, degenerated Achilles tendon tissues (HE, 400X); (C) AT group at the 6th week after operation, cartilage cells seen inside degenerated Achilles tendon (HE, 400X); (D) AT group at the 10th week after operation, mature bone tissues, trabecular structure and marrow cavity could be seen (HE, 400X); (E) AT group at the 6th week after operation, bone lacuna and trabecular bone structure could be seen (Masson, 400X); (F) AT group at the 10th week after operation, mature lamellar bone structure could be seen (Masson, 400X).

Mentions: At the 6th and the 10th postoperative week, histological examination on the sham and PAT groups samples, demonstrated no cartilage or bone formation (Figure 2-A, B). In the AT group at 6th postoperative week, cartilage cells with bone lacuna and trabecular bone structure were observed (Figure 2-C, E); at the 10th week after operation, mature bone tissues, trabecular structure, marrow cavity and lamellar bone structure could be seen inside the slices (Figure 2-D, F).


Establishment of heterotopic ossification via sharp instrument injury in rats
Histological examination results: (A) Sham group at the 10th week after operation, normal Achilles tendon tissues (HE, 400X); (B) PAT group at the 10th week after operation, degenerated Achilles tendon tissues (HE, 400X); (C) AT group at the 6th week after operation, cartilage cells seen inside degenerated Achilles tendon (HE, 400X); (D) AT group at the 10th week after operation, mature bone tissues, trabecular structure and marrow cavity could be seen (HE, 400X); (E) AT group at the 6th week after operation, bone lacuna and trabecular bone structure could be seen (Masson, 400X); (F) AT group at the 10th week after operation, mature lamellar bone structure could be seen (Masson, 400X).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383774&req=5

Figure 2: Histological examination results: (A) Sham group at the 10th week after operation, normal Achilles tendon tissues (HE, 400X); (B) PAT group at the 10th week after operation, degenerated Achilles tendon tissues (HE, 400X); (C) AT group at the 6th week after operation, cartilage cells seen inside degenerated Achilles tendon (HE, 400X); (D) AT group at the 10th week after operation, mature bone tissues, trabecular structure and marrow cavity could be seen (HE, 400X); (E) AT group at the 6th week after operation, bone lacuna and trabecular bone structure could be seen (Masson, 400X); (F) AT group at the 10th week after operation, mature lamellar bone structure could be seen (Masson, 400X).
Mentions: At the 6th and the 10th postoperative week, histological examination on the sham and PAT groups samples, demonstrated no cartilage or bone formation (Figure 2-A, B). In the AT group at 6th postoperative week, cartilage cells with bone lacuna and trabecular bone structure were observed (Figure 2-C, E); at the 10th week after operation, mature bone tissues, trabecular structure, marrow cavity and lamellar bone structure could be seen inside the slices (Figure 2-D, F).

View Article: PubMed Central - PubMed

ABSTRACT

Objective:: To establish an animal model for heterotopic ossification (HO) induced by sharp instrument injury in Sprague-Dawley (SD) rat and to investigate its possible mechanism.

Materials and methods:: A total of 48 male SD rats were divided into 3 groups (n=16). In sham group, incision and suture were performed only in the left leg. Partial tenotomy was performed in the left Achilles tendons in the PAT group. In Achilles’ tenotomy (AT) group, tenotomy was performed in the left Achilles tendons to establish animal model of EO. X-ray and histological examinations were made at 6 and 10 weeks after operation.

Results:: No HO occurred in the sham and PAT groups. In AT group, X–ray results on 4 rats showed cartilage and bone formation while the remaining 4 rats showed chondrification in histological examination at 6 weeks after operation. At 10 weeks all rats showed bone formation with trabecular bone. This kind of HO usually develops through a process of endochondral ossification.

Conclusion:: Tenotomy is a simple, effective and feasible method to induce HO.

No MeSH data available.


Related in: MedlinePlus