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Long-term wheel-running can prevent deterioration of bone properties in diabetes mellitus model rats

View Article: PubMed Central - PubMed

ABSTRACT

Objectives:: The purpose of this study was to examine the effects of long-term wheel-running on tibia bone properties in T2DM Otsuka Long-Evans Tokushima Fatty (OLETF) rats.

Methods:: Ten five-week-old male OLETF rats were used as experimental animals and 5 Long-Evans Tokushima Otsuka (LETO) rats as controls. Half of OLETF rats performed daily voluntary wheel-running for 17 months (OLETF-EXE), while neither the remainder of OLETF nor LETO rats had exercise. At the end of experiment, in addition to serum biochemical and bone formation/resorption marker analyses, bone mass, trabecular bone microarchitecture and cortical bone geometry were analyzed in left tibia, and bone mechanical strength of right tibia was measured.

Results:: Tibia bone mass, trabecular bone microarchitecture, cortical bone geometry and bone mechanical strength deteriorated in diabetic OLETF rats. However, such deterioration was obviously attenuated in OLETF-EXE rats, which maintained normal levels of blood glucose, HbA1c and blood urea nitrogen.

Conclusions:: Daily wheel-running could prevent the deterioration of bone properties in OLETF rats. This would be induced mainly by suppressing the development of T2DM. Regular physical exercise may be a potent strategy for preventing not only the development of diabetes but also the deterioration of bone properties in patients with chronic T2DM.

No MeSH data available.


Serum levels of bone specific alkaline phosphatase (A) and tartrate-resistant acid phosphatase-5b (B). BAP, bone specific alkaline phosphatase; TRACP-5b, tartrate-resistant acid phosphatase-5b. Symbols represent data from individual animals. More than 1.4 mU/mL of serum TRACP-5b concentration could be detected with the present ELISA. *Significantly different from the LETO group (p<0.05). N=5 in each group.
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Figure 5: Serum levels of bone specific alkaline phosphatase (A) and tartrate-resistant acid phosphatase-5b (B). BAP, bone specific alkaline phosphatase; TRACP-5b, tartrate-resistant acid phosphatase-5b. Symbols represent data from individual animals. More than 1.4 mU/mL of serum TRACP-5b concentration could be detected with the present ELISA. *Significantly different from the LETO group (p<0.05). N=5 in each group.

Mentions: Serum levels of the bone formation marker BAP were significantly lower not only in the OLETF group but also in the OLETF-EXE group than in the LETO group (Figure 5). On the other hand, the present ELISA was unable to detect the bone resorption marker TRACP-5b in 4 serum samples from the OLETF-EXE group as well as from the LETO group, but was able to detect it in 3 serum samples from the OLETF group (Figure 5).


Long-term wheel-running can prevent deterioration of bone properties in diabetes mellitus model rats
Serum levels of bone specific alkaline phosphatase (A) and tartrate-resistant acid phosphatase-5b (B). BAP, bone specific alkaline phosphatase; TRACP-5b, tartrate-resistant acid phosphatase-5b. Symbols represent data from individual animals. More than 1.4 mU/mL of serum TRACP-5b concentration could be detected with the present ELISA. *Significantly different from the LETO group (p<0.05). N=5 in each group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383771&req=5

Figure 5: Serum levels of bone specific alkaline phosphatase (A) and tartrate-resistant acid phosphatase-5b (B). BAP, bone specific alkaline phosphatase; TRACP-5b, tartrate-resistant acid phosphatase-5b. Symbols represent data from individual animals. More than 1.4 mU/mL of serum TRACP-5b concentration could be detected with the present ELISA. *Significantly different from the LETO group (p<0.05). N=5 in each group.
Mentions: Serum levels of the bone formation marker BAP were significantly lower not only in the OLETF group but also in the OLETF-EXE group than in the LETO group (Figure 5). On the other hand, the present ELISA was unable to detect the bone resorption marker TRACP-5b in 4 serum samples from the OLETF-EXE group as well as from the LETO group, but was able to detect it in 3 serum samples from the OLETF group (Figure 5).

View Article: PubMed Central - PubMed

ABSTRACT

Objectives:: The purpose of this study was to examine the effects of long-term wheel-running on tibia bone properties in T2DM Otsuka Long-Evans Tokushima Fatty (OLETF) rats.

Methods:: Ten five-week-old male OLETF rats were used as experimental animals and 5 Long-Evans Tokushima Otsuka (LETO) rats as controls. Half of OLETF rats performed daily voluntary wheel-running for 17 months (OLETF-EXE), while neither the remainder of OLETF nor LETO rats had exercise. At the end of experiment, in addition to serum biochemical and bone formation/resorption marker analyses, bone mass, trabecular bone microarchitecture and cortical bone geometry were analyzed in left tibia, and bone mechanical strength of right tibia was measured.

Results:: Tibia bone mass, trabecular bone microarchitecture, cortical bone geometry and bone mechanical strength deteriorated in diabetic OLETF rats. However, such deterioration was obviously attenuated in OLETF-EXE rats, which maintained normal levels of blood glucose, HbA1c and blood urea nitrogen.

Conclusions:: Daily wheel-running could prevent the deterioration of bone properties in OLETF rats. This would be induced mainly by suppressing the development of T2DM. Regular physical exercise may be a potent strategy for preventing not only the development of diabetes but also the deterioration of bone properties in patients with chronic T2DM.

No MeSH data available.