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Long-term wheel-running can prevent deterioration of bone properties in diabetes mellitus model rats

View Article: PubMed Central - PubMed

ABSTRACT

Objectives:: The purpose of this study was to examine the effects of long-term wheel-running on tibia bone properties in T2DM Otsuka Long-Evans Tokushima Fatty (OLETF) rats.

Methods:: Ten five-week-old male OLETF rats were used as experimental animals and 5 Long-Evans Tokushima Otsuka (LETO) rats as controls. Half of OLETF rats performed daily voluntary wheel-running for 17 months (OLETF-EXE), while neither the remainder of OLETF nor LETO rats had exercise. At the end of experiment, in addition to serum biochemical and bone formation/resorption marker analyses, bone mass, trabecular bone microarchitecture and cortical bone geometry were analyzed in left tibia, and bone mechanical strength of right tibia was measured.

Results:: Tibia bone mass, trabecular bone microarchitecture, cortical bone geometry and bone mechanical strength deteriorated in diabetic OLETF rats. However, such deterioration was obviously attenuated in OLETF-EXE rats, which maintained normal levels of blood glucose, HbA1c and blood urea nitrogen.

Conclusions:: Daily wheel-running could prevent the deterioration of bone properties in OLETF rats. This would be induced mainly by suppressing the development of T2DM. Regular physical exercise may be a potent strategy for preventing not only the development of diabetes but also the deterioration of bone properties in patients with chronic T2DM.

No MeSH data available.


Related in: MedlinePlus

Trabecular bone microarchitecture parameters in tibia metaphysis. A, tissue volume (TV); B, bone volume (BV); C, bone volume fraction (BV/TV); D, trabecular thickness (Tb.Th); E, trabecular number (Tb.N); F, trabecular separation (Tb.Sp); G, connectivity density (Conn.D). *Significantly different from the LETO group (p<0.05), †Significantly different from the OLETF group (p<0.05). N=5 in each group.
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Figure 2: Trabecular bone microarchitecture parameters in tibia metaphysis. A, tissue volume (TV); B, bone volume (BV); C, bone volume fraction (BV/TV); D, trabecular thickness (Tb.Th); E, trabecular number (Tb.N); F, trabecular separation (Tb.Sp); G, connectivity density (Conn.D). *Significantly different from the LETO group (p<0.05), †Significantly different from the OLETF group (p<0.05). N=5 in each group.

Mentions: In trabecular bone microarchitecture parameters of the tibia metaphysis, BV, BV/TV, Tb.N and Conn.D were considerably higher in the OLETF-EXE and LETO groups than in the OLETF group, though TV in the OLETF-EXE and OLETF groups were the lowest and medium, respectively (Figure 2). To the contrary, Tb.Th was significantly lower in the LETO group than in the OLETF group (Figure 2). There was no significant difference in Tb.Sp among the 3 groups. In cortical bone geometry parameters of the tibia diaphysis, on the other hand, CV, CV/AV, Ct.Th and Ct.Ar were significantly lower in the OLETF-EXE group, as well as in the OLETF group, than in the LETO group (Figure 3). However, CV/AV and Ct.Th in the OLETF-EXE group were significantly higher than those of the OLETF group. To the contrary, MV in the OLETF group was prominent as compared with two other groups. In addition, the OLETF-EXE group showed a decreased Ec.Pm, irrespective of similar Ps.Pm, compared with two other groups (Figure 3).


Long-term wheel-running can prevent deterioration of bone properties in diabetes mellitus model rats
Trabecular bone microarchitecture parameters in tibia metaphysis. A, tissue volume (TV); B, bone volume (BV); C, bone volume fraction (BV/TV); D, trabecular thickness (Tb.Th); E, trabecular number (Tb.N); F, trabecular separation (Tb.Sp); G, connectivity density (Conn.D). *Significantly different from the LETO group (p<0.05), †Significantly different from the OLETF group (p<0.05). N=5 in each group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383771&req=5

Figure 2: Trabecular bone microarchitecture parameters in tibia metaphysis. A, tissue volume (TV); B, bone volume (BV); C, bone volume fraction (BV/TV); D, trabecular thickness (Tb.Th); E, trabecular number (Tb.N); F, trabecular separation (Tb.Sp); G, connectivity density (Conn.D). *Significantly different from the LETO group (p<0.05), †Significantly different from the OLETF group (p<0.05). N=5 in each group.
Mentions: In trabecular bone microarchitecture parameters of the tibia metaphysis, BV, BV/TV, Tb.N and Conn.D were considerably higher in the OLETF-EXE and LETO groups than in the OLETF group, though TV in the OLETF-EXE and OLETF groups were the lowest and medium, respectively (Figure 2). To the contrary, Tb.Th was significantly lower in the LETO group than in the OLETF group (Figure 2). There was no significant difference in Tb.Sp among the 3 groups. In cortical bone geometry parameters of the tibia diaphysis, on the other hand, CV, CV/AV, Ct.Th and Ct.Ar were significantly lower in the OLETF-EXE group, as well as in the OLETF group, than in the LETO group (Figure 3). However, CV/AV and Ct.Th in the OLETF-EXE group were significantly higher than those of the OLETF group. To the contrary, MV in the OLETF group was prominent as compared with two other groups. In addition, the OLETF-EXE group showed a decreased Ec.Pm, irrespective of similar Ps.Pm, compared with two other groups (Figure 3).

View Article: PubMed Central - PubMed

ABSTRACT

Objectives:: The purpose of this study was to examine the effects of long-term wheel-running on tibia bone properties in T2DM Otsuka Long-Evans Tokushima Fatty (OLETF) rats.

Methods:: Ten five-week-old male OLETF rats were used as experimental animals and 5 Long-Evans Tokushima Otsuka (LETO) rats as controls. Half of OLETF rats performed daily voluntary wheel-running for 17 months (OLETF-EXE), while neither the remainder of OLETF nor LETO rats had exercise. At the end of experiment, in addition to serum biochemical and bone formation/resorption marker analyses, bone mass, trabecular bone microarchitecture and cortical bone geometry were analyzed in left tibia, and bone mechanical strength of right tibia was measured.

Results:: Tibia bone mass, trabecular bone microarchitecture, cortical bone geometry and bone mechanical strength deteriorated in diabetic OLETF rats. However, such deterioration was obviously attenuated in OLETF-EXE rats, which maintained normal levels of blood glucose, HbA1c and blood urea nitrogen.

Conclusions:: Daily wheel-running could prevent the deterioration of bone properties in OLETF rats. This would be induced mainly by suppressing the development of T2DM. Regular physical exercise may be a potent strategy for preventing not only the development of diabetes but also the deterioration of bone properties in patients with chronic T2DM.

No MeSH data available.


Related in: MedlinePlus