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Long-term wheel-running can prevent deterioration of bone properties in diabetes mellitus model rats

View Article: PubMed Central - PubMed

ABSTRACT

Objectives:: The purpose of this study was to examine the effects of long-term wheel-running on tibia bone properties in T2DM Otsuka Long-Evans Tokushima Fatty (OLETF) rats.

Methods:: Ten five-week-old male OLETF rats were used as experimental animals and 5 Long-Evans Tokushima Otsuka (LETO) rats as controls. Half of OLETF rats performed daily voluntary wheel-running for 17 months (OLETF-EXE), while neither the remainder of OLETF nor LETO rats had exercise. At the end of experiment, in addition to serum biochemical and bone formation/resorption marker analyses, bone mass, trabecular bone microarchitecture and cortical bone geometry were analyzed in left tibia, and bone mechanical strength of right tibia was measured.

Results:: Tibia bone mass, trabecular bone microarchitecture, cortical bone geometry and bone mechanical strength deteriorated in diabetic OLETF rats. However, such deterioration was obviously attenuated in OLETF-EXE rats, which maintained normal levels of blood glucose, HbA1c and blood urea nitrogen.

Conclusions:: Daily wheel-running could prevent the deterioration of bone properties in OLETF rats. This would be induced mainly by suppressing the development of T2DM. Regular physical exercise may be a potent strategy for preventing not only the development of diabetes but also the deterioration of bone properties in patients with chronic T2DM.

No MeSH data available.


Representative micro-CT images of tibia trabecular/cortical bone transverse sections. Trabecular bone of proximal tibia metaphysis in the LETO (A), OLETF (B) and OLETF-EXE (C) rat. Cortical bone of tibia diaphysis in the LETO (D), OLETF (E) and OLETF-EXE (F) rat. Bar=1 mm.
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Figure 1: Representative micro-CT images of tibia trabecular/cortical bone transverse sections. Trabecular bone of proximal tibia metaphysis in the LETO (A), OLETF (B) and OLETF-EXE (C) rat. Cortical bone of tibia diaphysis in the LETO (D), OLETF (E) and OLETF-EXE (F) rat. Bar=1 mm.

Mentions: Figure 1 shows cortical and trabecular bone transverse sections of tibia ROI in the 3 groups. Trabecular bone connectivity at metaphysis of the proximal tibia appears to be relatively well maintained in the OLETF-EXE group (Figure 1C), whereas trabecular bone of the OLETF groups is disconnected (Figure 1B). On the other hand, the micro-CT images of cortical bone at diaphysis of tibia show that bone marrow area is slightly increased and bone is somewhat thinner in the OLETF group (Figure 1E), as compared with in the OLETF-EXE group (Figure 1F). Thus, the preventive effects of wheel-running exercise on bone deterioration were evident in the T2DM rat model OLETF.


Long-term wheel-running can prevent deterioration of bone properties in diabetes mellitus model rats
Representative micro-CT images of tibia trabecular/cortical bone transverse sections. Trabecular bone of proximal tibia metaphysis in the LETO (A), OLETF (B) and OLETF-EXE (C) rat. Cortical bone of tibia diaphysis in the LETO (D), OLETF (E) and OLETF-EXE (F) rat. Bar=1 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383771&req=5

Figure 1: Representative micro-CT images of tibia trabecular/cortical bone transverse sections. Trabecular bone of proximal tibia metaphysis in the LETO (A), OLETF (B) and OLETF-EXE (C) rat. Cortical bone of tibia diaphysis in the LETO (D), OLETF (E) and OLETF-EXE (F) rat. Bar=1 mm.
Mentions: Figure 1 shows cortical and trabecular bone transverse sections of tibia ROI in the 3 groups. Trabecular bone connectivity at metaphysis of the proximal tibia appears to be relatively well maintained in the OLETF-EXE group (Figure 1C), whereas trabecular bone of the OLETF groups is disconnected (Figure 1B). On the other hand, the micro-CT images of cortical bone at diaphysis of tibia show that bone marrow area is slightly increased and bone is somewhat thinner in the OLETF group (Figure 1E), as compared with in the OLETF-EXE group (Figure 1F). Thus, the preventive effects of wheel-running exercise on bone deterioration were evident in the T2DM rat model OLETF.

View Article: PubMed Central - PubMed

ABSTRACT

Objectives:: The purpose of this study was to examine the effects of long-term wheel-running on tibia bone properties in T2DM Otsuka Long-Evans Tokushima Fatty (OLETF) rats.

Methods:: Ten five-week-old male OLETF rats were used as experimental animals and 5 Long-Evans Tokushima Otsuka (LETO) rats as controls. Half of OLETF rats performed daily voluntary wheel-running for 17 months (OLETF-EXE), while neither the remainder of OLETF nor LETO rats had exercise. At the end of experiment, in addition to serum biochemical and bone formation/resorption marker analyses, bone mass, trabecular bone microarchitecture and cortical bone geometry were analyzed in left tibia, and bone mechanical strength of right tibia was measured.

Results:: Tibia bone mass, trabecular bone microarchitecture, cortical bone geometry and bone mechanical strength deteriorated in diabetic OLETF rats. However, such deterioration was obviously attenuated in OLETF-EXE rats, which maintained normal levels of blood glucose, HbA1c and blood urea nitrogen.

Conclusions:: Daily wheel-running could prevent the deterioration of bone properties in OLETF rats. This would be induced mainly by suppressing the development of T2DM. Regular physical exercise may be a potent strategy for preventing not only the development of diabetes but also the deterioration of bone properties in patients with chronic T2DM.

No MeSH data available.