Limits...
Serial Changes of Heat Shock Protein 70 and Interleukin-8 in Burn Blister Fluid

View Article: PubMed Central - PubMed

ABSTRACT

Background: It has been reported that heat shock protein 70 (HSP70) and interleukin-8 (IL-8) play an important role in cells during the wound healing process. However, there has been no report on the effect of HSP70 and IL-8 on the blisters of burn patients.

Objective: This study aimed to evaluate the serial quantitative changes of HSP70 and IL-8 in burn blisters.

Methods: Twenty-five burn patients were included, for a total of 36 cases: twenty cases on the first day, six cases on the second, five cases on the third, three cases on the fourth, and two cases on the fifth. A correlation analysis was performed to determine the relationship between the concentration of HSP70 and IL-8 and the length of the treatment period.

Results: The HSP70 concentration was the highest on the first day, after which it decreased down to near zero. Most HSP70 was generated during the first 12 hours after the burn accident. There was no correlation between the concentration of HSP70 on the first day and the length of the treatment period. No measurable concentration of IL-8 was detected before 5 hours, but the concentration started to increase after 11 hours. The peak value was measured on the fourth day.

Conclusion: While HSP70 increased in the first few hours and decreased afterwards, IL-8 was produced after 11 hours and increased afterward in burn blister fluid. These findings provide new evidence on serial changes of inflammatory mediators in burn blister fluid.

No MeSH data available.


Related in: MedlinePlus

(A) Variation of the interleukin-8 (IL-8) concentration within partial thickness burn blister over the time. The IL-8 concentration was measured to be the highest on the fourth day. (B) The IL-8 concentration within partial thickness burn blister up to 12 hours after damage. IL-8 was not observed up to 5 hours of the occurrence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5383745&req=5

Figure 2: (A) Variation of the interleukin-8 (IL-8) concentration within partial thickness burn blister over the time. The IL-8 concentration was measured to be the highest on the fourth day. (B) The IL-8 concentration within partial thickness burn blister up to 12 hours after damage. IL-8 was not observed up to 5 hours of the occurrence.

Mentions: The concentration of IL-8 was measured from the first day to the fifth day. The median concentration of IL-8 was 0 pg/dl on the first day, 1,477.245 pg/dl (IQR, 733.622~2,302.500 pg/dl) on the second day, 511.429 pg/dl (IQR, 322.483~4,021.122 pg/dl) on the third day, 1,653.010 pg/dl (IQR, 584.388~2,187.322 pg/dl) on the fourth day, and 1,473.6735 pg/dl (IQR, 1,006.071~1,707.477 pg/dl) on the fifth day (Fig. 2A). When the concentration of IL-8 within the first 12 hours was analyzed, IL-8 was not detected up to 5 hours after the damage (Fig. 2B). There was no correlation between the concentration of IL-8 and treatment period (rho=−0.326, p=0.053).


Serial Changes of Heat Shock Protein 70 and Interleukin-8 in Burn Blister Fluid
(A) Variation of the interleukin-8 (IL-8) concentration within partial thickness burn blister over the time. The IL-8 concentration was measured to be the highest on the fourth day. (B) The IL-8 concentration within partial thickness burn blister up to 12 hours after damage. IL-8 was not observed up to 5 hours of the occurrence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383745&req=5

Figure 2: (A) Variation of the interleukin-8 (IL-8) concentration within partial thickness burn blister over the time. The IL-8 concentration was measured to be the highest on the fourth day. (B) The IL-8 concentration within partial thickness burn blister up to 12 hours after damage. IL-8 was not observed up to 5 hours of the occurrence.
Mentions: The concentration of IL-8 was measured from the first day to the fifth day. The median concentration of IL-8 was 0 pg/dl on the first day, 1,477.245 pg/dl (IQR, 733.622~2,302.500 pg/dl) on the second day, 511.429 pg/dl (IQR, 322.483~4,021.122 pg/dl) on the third day, 1,653.010 pg/dl (IQR, 584.388~2,187.322 pg/dl) on the fourth day, and 1,473.6735 pg/dl (IQR, 1,006.071~1,707.477 pg/dl) on the fifth day (Fig. 2A). When the concentration of IL-8 within the first 12 hours was analyzed, IL-8 was not detected up to 5 hours after the damage (Fig. 2B). There was no correlation between the concentration of IL-8 and treatment period (rho=−0.326, p=0.053).

View Article: PubMed Central - PubMed

ABSTRACT

Background: It has been reported that heat shock protein 70 (HSP70) and interleukin-8 (IL-8) play an important role in cells during the wound healing process. However, there has been no report on the effect of HSP70 and IL-8 on the blisters of burn patients.

Objective: This study aimed to evaluate the serial quantitative changes of HSP70 and IL-8 in burn blisters.

Methods: Twenty-five burn patients were included, for a total of 36 cases: twenty cases on the first day, six cases on the second, five cases on the third, three cases on the fourth, and two cases on the fifth. A correlation analysis was performed to determine the relationship between the concentration of HSP70 and IL-8 and the length of the treatment period.

Results: The HSP70 concentration was the highest on the first day, after which it decreased down to near zero. Most HSP70 was generated during the first 12 hours after the burn accident. There was no correlation between the concentration of HSP70 on the first day and the length of the treatment period. No measurable concentration of IL-8 was detected before 5 hours, but the concentration started to increase after 11 hours. The peak value was measured on the fourth day.

Conclusion: While HSP70 increased in the first few hours and decreased afterwards, IL-8 was produced after 11 hours and increased afterward in burn blister fluid. These findings provide new evidence on serial changes of inflammatory mediators in burn blister fluid.

No MeSH data available.


Related in: MedlinePlus