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Serial Changes of Heat Shock Protein 70 and Interleukin-8 in Burn Blister Fluid

View Article: PubMed Central - PubMed

ABSTRACT

Background: It has been reported that heat shock protein 70 (HSP70) and interleukin-8 (IL-8) play an important role in cells during the wound healing process. However, there has been no report on the effect of HSP70 and IL-8 on the blisters of burn patients.

Objective: This study aimed to evaluate the serial quantitative changes of HSP70 and IL-8 in burn blisters.

Methods: Twenty-five burn patients were included, for a total of 36 cases: twenty cases on the first day, six cases on the second, five cases on the third, three cases on the fourth, and two cases on the fifth. A correlation analysis was performed to determine the relationship between the concentration of HSP70 and IL-8 and the length of the treatment period.

Results: The HSP70 concentration was the highest on the first day, after which it decreased down to near zero. Most HSP70 was generated during the first 12 hours after the burn accident. There was no correlation between the concentration of HSP70 on the first day and the length of the treatment period. No measurable concentration of IL-8 was detected before 5 hours, but the concentration started to increase after 11 hours. The peak value was measured on the fourth day.

Conclusion: While HSP70 increased in the first few hours and decreased afterwards, IL-8 was produced after 11 hours and increased afterward in burn blister fluid. These findings provide new evidence on serial changes of inflammatory mediators in burn blister fluid.

No MeSH data available.


Related in: MedlinePlus

(A) The heat shock protein (HSP) concentration variation within partial thickness burn blister. The HSP70 concentration was the highest on the first day (up to 12 hours after the damage). Then, the value drastically dropped down to near zero on the second day. There was a small increased on the third day and no significant amount was found on the fourth and fifth day. (B) The concentration of HSP70 within partial thickness burn blister of up to 12 hours after damage. The HSP70 concentration after one hour of the damage was measured to be the highest. After one hour, the concentration drops rapidly.
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Figure 1: (A) The heat shock protein (HSP) concentration variation within partial thickness burn blister. The HSP70 concentration was the highest on the first day (up to 12 hours after the damage). Then, the value drastically dropped down to near zero on the second day. There was a small increased on the third day and no significant amount was found on the fourth and fifth day. (B) The concentration of HSP70 within partial thickness burn blister of up to 12 hours after damage. The HSP70 concentration after one hour of the damage was measured to be the highest. After one hour, the concentration drops rapidly.

Mentions: The median concentration of HSP70 was the highest on the first day (median, 5.555 ng/dl; IQR, 1.602~12.164 ng/dl). On the second day and third day, the median values were 0.038 ng/dl (IQR, 0.007~0.058 ng/dl) and 0.170 ng/dl (IQR, 0.058~0.552 ng/dl), respectively. HSP70 was not detected on the fourth and the fifth days (Fig. 1A). The majority of the HSP70 was found in the first 12 hours. The highest median HSP70 value was measured 1 hour after the damage, at 9.261 ng/dl (IQR, 7.347~15.345 ng/dl). After 1 hour, the concentration decreased rapidly: 7.285 ng/dl (IQR, 1.732~14.515 ng/dl) after 2 hours, 2.038 ng/dl after 4 hours, 0 ng/dl after 5 hours, 1.264 ng/dl after 11 hours, and 0.182 ng/dl after 12 hours (Fig. 1B). There was no correlation between the concentration of HSP70 and treatment period during the first 12 hours (rho=−0.316, p=0.175).


Serial Changes of Heat Shock Protein 70 and Interleukin-8 in Burn Blister Fluid
(A) The heat shock protein (HSP) concentration variation within partial thickness burn blister. The HSP70 concentration was the highest on the first day (up to 12 hours after the damage). Then, the value drastically dropped down to near zero on the second day. There was a small increased on the third day and no significant amount was found on the fourth and fifth day. (B) The concentration of HSP70 within partial thickness burn blister of up to 12 hours after damage. The HSP70 concentration after one hour of the damage was measured to be the highest. After one hour, the concentration drops rapidly.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383745&req=5

Figure 1: (A) The heat shock protein (HSP) concentration variation within partial thickness burn blister. The HSP70 concentration was the highest on the first day (up to 12 hours after the damage). Then, the value drastically dropped down to near zero on the second day. There was a small increased on the third day and no significant amount was found on the fourth and fifth day. (B) The concentration of HSP70 within partial thickness burn blister of up to 12 hours after damage. The HSP70 concentration after one hour of the damage was measured to be the highest. After one hour, the concentration drops rapidly.
Mentions: The median concentration of HSP70 was the highest on the first day (median, 5.555 ng/dl; IQR, 1.602~12.164 ng/dl). On the second day and third day, the median values were 0.038 ng/dl (IQR, 0.007~0.058 ng/dl) and 0.170 ng/dl (IQR, 0.058~0.552 ng/dl), respectively. HSP70 was not detected on the fourth and the fifth days (Fig. 1A). The majority of the HSP70 was found in the first 12 hours. The highest median HSP70 value was measured 1 hour after the damage, at 9.261 ng/dl (IQR, 7.347~15.345 ng/dl). After 1 hour, the concentration decreased rapidly: 7.285 ng/dl (IQR, 1.732~14.515 ng/dl) after 2 hours, 2.038 ng/dl after 4 hours, 0 ng/dl after 5 hours, 1.264 ng/dl after 11 hours, and 0.182 ng/dl after 12 hours (Fig. 1B). There was no correlation between the concentration of HSP70 and treatment period during the first 12 hours (rho=−0.316, p=0.175).

View Article: PubMed Central - PubMed

ABSTRACT

Background: It has been reported that heat shock protein 70 (HSP70) and interleukin-8 (IL-8) play an important role in cells during the wound healing process. However, there has been no report on the effect of HSP70 and IL-8 on the blisters of burn patients.

Objective: This study aimed to evaluate the serial quantitative changes of HSP70 and IL-8 in burn blisters.

Methods: Twenty-five burn patients were included, for a total of 36 cases: twenty cases on the first day, six cases on the second, five cases on the third, three cases on the fourth, and two cases on the fifth. A correlation analysis was performed to determine the relationship between the concentration of HSP70 and IL-8 and the length of the treatment period.

Results: The HSP70 concentration was the highest on the first day, after which it decreased down to near zero. Most HSP70 was generated during the first 12 hours after the burn accident. There was no correlation between the concentration of HSP70 on the first day and the length of the treatment period. No measurable concentration of IL-8 was detected before 5 hours, but the concentration started to increase after 11 hours. The peak value was measured on the fourth day.

Conclusion: While HSP70 increased in the first few hours and decreased afterwards, IL-8 was produced after 11 hours and increased afterward in burn blister fluid. These findings provide new evidence on serial changes of inflammatory mediators in burn blister fluid.

No MeSH data available.


Related in: MedlinePlus