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The Translation Initiation Factor 1A ( TheIF1A ) from Tamarix hispida Is Regulated by a Dof Transcription Factor and Increased Abiotic Stress Tolerance

View Article: PubMed Central - PubMed

ABSTRACT

Eukaryotic translation initiation factor 1A (eIF1A) functions as an mRNA scanner and AUG initiation codon locator. However, few studies have clarified the role of eIF1A in abiotic stress. In this study, we cloned eIF1A (TheIF1A) from Tamarix hispida and found its expression to be induced by NaCl and polyethylene glycol (PEG) in roots, stems, and leaves. Compared to control, TheIF1A root expression was increased 187.63-fold when exposed to NaCl for 6 h, suggesting a potential abiotic stress response for this gene. Furthermore, transgenic tobacco plants overexpressing TheIF1A exhibited enhanced seed germination and a higher total chlorophyll content under salt and mannitol stresses. Increased superoxide dismutase, peroxidase, glutathione transferase and glutathione peroxidase activities, as well as decreased electrolyte leakage rates and malondialdehyde contents, were observed in TheIF1A-transgenic tobacco and T. hispida seedlings under salt and mannitol stresses. Histochemical staining suggested that TheIF1A improves reactive oxygen species (ROS) scavenging in plants. Moreover, TheIF1A may regulate expression of stress-related genes, including TOBLTP, GST, MnSOD, NtMPK9, poxN1, and CDPK15. Moreover, a 1352-bp promoter fragment of TheIF1A was isolated, and cis-elements were identified. Yeast one-hybrid assays showed that ThDof can specifically bind to the Dof motif present in the promoter. In addition, ThDof showed expression patterns similar to those of TheIF1A under NaCl and PEG stresses. These findings suggest the potential mechanism and physiological roles of TheIF1A. ThDof may be an upstream regulator of TheIF1A, and TheIF1A may function as a stress response regulator to improve plant salt and osmotic stress tolerance via regulation of associated enzymes and ROS scavenging, thereby reducing cell damage under stress conditions.

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TheIF1A and ThDof expression patterns in different organs of Tamarix hispida in response to various treatments. The relative expression level = transcription level under stress treatment/transcription level under control condition (0 h). And all the expression leves were log2 transformed. β-Actin (FJ618517), α-tubulin (FJ618518), and β-tubulin (FJ618519) were used as reference genes. Error bars were obtained from three replicates of real-time PCR, and every replicate included at least 20 seedlings used as biological replicates. (A) 0.4 M NaCl stress; (B) 20% (w/v) PEG6000 stress.
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Figure 1: TheIF1A and ThDof expression patterns in different organs of Tamarix hispida in response to various treatments. The relative expression level = transcription level under stress treatment/transcription level under control condition (0 h). And all the expression leves were log2 transformed. β-Actin (FJ618517), α-tubulin (FJ618518), and β-tubulin (FJ618519) were used as reference genes. Error bars were obtained from three replicates of real-time PCR, and every replicate included at least 20 seedlings used as biological replicates. (A) 0.4 M NaCl stress; (B) 20% (w/v) PEG6000 stress.

Mentions: The results of qRT-PCR showed TheIF1A to be induced in response to NaCl and polyethylene glycol (PEG) treatments. After NaCl treatment for 6 h, expression of TheIF1A in roots was increased by 187.6-fold compared to the control. At 48 h, TheIF1A expression was down-regulated in roots compared to the control but up-regulated by 1.97-fold in stems (Figure 1A). Under PEG stress, TheIF1A transcription was considerably higher in leaves than in roots and stems, with TheIF1A transcripts in leaves being 4.22-fold higher in roots and 2.43-fold higher in stems at 12 h (Figure 1B).


The Translation Initiation Factor 1A ( TheIF1A ) from Tamarix hispida Is Regulated by a Dof Transcription Factor and Increased Abiotic Stress Tolerance
TheIF1A and ThDof expression patterns in different organs of Tamarix hispida in response to various treatments. The relative expression level = transcription level under stress treatment/transcription level under control condition (0 h). And all the expression leves were log2 transformed. β-Actin (FJ618517), α-tubulin (FJ618518), and β-tubulin (FJ618519) were used as reference genes. Error bars were obtained from three replicates of real-time PCR, and every replicate included at least 20 seedlings used as biological replicates. (A) 0.4 M NaCl stress; (B) 20% (w/v) PEG6000 stress.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383729&req=5

Figure 1: TheIF1A and ThDof expression patterns in different organs of Tamarix hispida in response to various treatments. The relative expression level = transcription level under stress treatment/transcription level under control condition (0 h). And all the expression leves were log2 transformed. β-Actin (FJ618517), α-tubulin (FJ618518), and β-tubulin (FJ618519) were used as reference genes. Error bars were obtained from three replicates of real-time PCR, and every replicate included at least 20 seedlings used as biological replicates. (A) 0.4 M NaCl stress; (B) 20% (w/v) PEG6000 stress.
Mentions: The results of qRT-PCR showed TheIF1A to be induced in response to NaCl and polyethylene glycol (PEG) treatments. After NaCl treatment for 6 h, expression of TheIF1A in roots was increased by 187.6-fold compared to the control. At 48 h, TheIF1A expression was down-regulated in roots compared to the control but up-regulated by 1.97-fold in stems (Figure 1A). Under PEG stress, TheIF1A transcription was considerably higher in leaves than in roots and stems, with TheIF1A transcripts in leaves being 4.22-fold higher in roots and 2.43-fold higher in stems at 12 h (Figure 1B).

View Article: PubMed Central - PubMed

ABSTRACT

Eukaryotic translation initiation factor 1A (eIF1A) functions as an mRNA scanner and AUG initiation codon locator. However, few studies have clarified the role of eIF1A in abiotic stress. In this study, we cloned eIF1A (TheIF1A) from Tamarix hispida and found its expression to be induced by NaCl and polyethylene glycol (PEG) in roots, stems, and leaves. Compared to control, TheIF1A root expression was increased 187.63-fold when exposed to NaCl for 6 h, suggesting a potential abiotic stress response for this gene. Furthermore, transgenic tobacco plants overexpressing TheIF1A exhibited enhanced seed germination and a higher total chlorophyll content under salt and mannitol stresses. Increased superoxide dismutase, peroxidase, glutathione transferase and glutathione peroxidase activities, as well as decreased electrolyte leakage rates and malondialdehyde contents, were observed in TheIF1A-transgenic tobacco and T. hispida seedlings under salt and mannitol stresses. Histochemical staining suggested that TheIF1A improves reactive oxygen species (ROS) scavenging in plants. Moreover, TheIF1A may regulate expression of stress-related genes, including TOBLTP, GST, MnSOD, NtMPK9, poxN1, and CDPK15. Moreover, a 1352-bp promoter fragment of TheIF1A was isolated, and cis-elements were identified. Yeast one-hybrid assays showed that ThDof can specifically bind to the Dof motif present in the promoter. In addition, ThDof showed expression patterns similar to those of TheIF1A under NaCl and PEG stresses. These findings suggest the potential mechanism and physiological roles of TheIF1A. ThDof may be an upstream regulator of TheIF1A, and TheIF1A may function as a stress response regulator to improve plant salt and osmotic stress tolerance via regulation of associated enzymes and ROS scavenging, thereby reducing cell damage under stress conditions.

No MeSH data available.


Related in: MedlinePlus