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L -Theanine Content and Related Gene Expression: Novel Insights into Theanine Biosynthesis and Hydrolysis among Different Tea Plant ( Camellia sinensis L.) Tissues and Cultivars

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ABSTRACT

L-Theanine content has tissues and cultivars specificity in tea plant (Camellia sinensis L.), the correlations of theanine metabolic related genes expression profiles with theanine contents were explored in this study. L-theanine contents in the bud and 1st leaf, 2nd leaf, 3rd leaf, old leaf, stem, and lateral root were determined by HPLC from three C. sinensis cultivars, namely ‘Huangjinya’, ‘Anjibaicha’, and ‘Yingshuang’, respectively. The theanine contents in leaves and root of ‘Huangjinya’ were the highest, followed by ‘Anjibaicha’, and ‘Yingshuang’. The theanine contents in the leaves reduced as the leaf mature gradually, and in stem were the least. Seventeen genes encoding enzymes involved in theanine metabolism were identified from GenBank and our tea transcriptome database, including CsTS1, CsTS2, CsGS1, CsGS2, CsGOGAT-Fe, CsGOGAT-NAD(P)H, CsGDH1, CsGDH2, CsALT, CsSAMDC, CsADC, CsCuAO, CsPAO, CsNiR, CsNR, CsGGT1, and CsGGT3. The transcript profiles of those seventeen genes in the different tissues of three tea plant cultivars were analyzed comparatively. Among the different cultivars, the transcript levels of most selected genes in ‘Huangjinya’ were significantly higher than that in the ‘Anjibaicha’ and ‘Yingshuang’. Among the different tissues, the transcript levels of CsTS2, CsGS1, and CsGDH2 almost showed positive correlation with the theanine contents, while the other genes showed negative correlation with the theanine contents in most cases. The theanine contents showed correlations with related genes expression levels among cultivars and tissues of tea plant, and were determined by the integrated effect of the metabolic related genes.

No MeSH data available.


Expression levels of L-theanine metabolism pathway genes among C. sinensis cultivars ‘Chawansanhao’, ‘Ruchengmaoyecha’, and ‘Anjibaicha’.
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Figure 5: Expression levels of L-theanine metabolism pathway genes among C. sinensis cultivars ‘Chawansanhao’, ‘Ruchengmaoyecha’, and ‘Anjibaicha’.

Mentions: Then the unigene expression changes in different tea plant cultivars after quantification using the RPKM values (Figure 5 and Supplementary Table S5). Among the selected 17 unigenes, except the CsGDH1, CsGGT1, CsGGT3, and CsNR, most of them expresses lower level in the C. sinensis ‘Ruchengmaoyecha’ (large-leaf cultivar) than that in the other two cultivars C. sinensis ‘Chawansanhao’ and ‘Anjibaicha’ (small-leaf cultivar). It may be deduced that the expression of metabolic genes involved in theanine metabolism were correlated with theanine content to a large extent.


L -Theanine Content and Related Gene Expression: Novel Insights into Theanine Biosynthesis and Hydrolysis among Different Tea Plant ( Camellia sinensis L.) Tissues and Cultivars
Expression levels of L-theanine metabolism pathway genes among C. sinensis cultivars ‘Chawansanhao’, ‘Ruchengmaoyecha’, and ‘Anjibaicha’.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383724&req=5

Figure 5: Expression levels of L-theanine metabolism pathway genes among C. sinensis cultivars ‘Chawansanhao’, ‘Ruchengmaoyecha’, and ‘Anjibaicha’.
Mentions: Then the unigene expression changes in different tea plant cultivars after quantification using the RPKM values (Figure 5 and Supplementary Table S5). Among the selected 17 unigenes, except the CsGDH1, CsGGT1, CsGGT3, and CsNR, most of them expresses lower level in the C. sinensis ‘Ruchengmaoyecha’ (large-leaf cultivar) than that in the other two cultivars C. sinensis ‘Chawansanhao’ and ‘Anjibaicha’ (small-leaf cultivar). It may be deduced that the expression of metabolic genes involved in theanine metabolism were correlated with theanine content to a large extent.

View Article: PubMed Central - PubMed

ABSTRACT

L-Theanine content has tissues and cultivars specificity in tea plant (Camellia sinensis L.), the correlations of theanine metabolic related genes expression profiles with theanine contents were explored in this study. L-theanine contents in the bud and 1st leaf, 2nd leaf, 3rd leaf, old leaf, stem, and lateral root were determined by HPLC from three C. sinensis cultivars, namely ‘Huangjinya’, ‘Anjibaicha’, and ‘Yingshuang’, respectively. The theanine contents in leaves and root of ‘Huangjinya’ were the highest, followed by ‘Anjibaicha’, and ‘Yingshuang’. The theanine contents in the leaves reduced as the leaf mature gradually, and in stem were the least. Seventeen genes encoding enzymes involved in theanine metabolism were identified from GenBank and our tea transcriptome database, including CsTS1, CsTS2, CsGS1, CsGS2, CsGOGAT-Fe, CsGOGAT-NAD(P)H, CsGDH1, CsGDH2, CsALT, CsSAMDC, CsADC, CsCuAO, CsPAO, CsNiR, CsNR, CsGGT1, and CsGGT3. The transcript profiles of those seventeen genes in the different tissues of three tea plant cultivars were analyzed comparatively. Among the different cultivars, the transcript levels of most selected genes in ‘Huangjinya’ were significantly higher than that in the ‘Anjibaicha’ and ‘Yingshuang’. Among the different tissues, the transcript levels of CsTS2, CsGS1, and CsGDH2 almost showed positive correlation with the theanine contents, while the other genes showed negative correlation with the theanine contents in most cases. The theanine contents showed correlations with related genes expression levels among cultivars and tissues of tea plant, and were determined by the integrated effect of the metabolic related genes.

No MeSH data available.