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L -Theanine Content and Related Gene Expression: Novel Insights into Theanine Biosynthesis and Hydrolysis among Different Tea Plant ( Camellia sinensis L.) Tissues and Cultivars

View Article: PubMed Central - PubMed

ABSTRACT

L-Theanine content has tissues and cultivars specificity in tea plant (Camellia sinensis L.), the correlations of theanine metabolic related genes expression profiles with theanine contents were explored in this study. L-theanine contents in the bud and 1st leaf, 2nd leaf, 3rd leaf, old leaf, stem, and lateral root were determined by HPLC from three C. sinensis cultivars, namely ‘Huangjinya’, ‘Anjibaicha’, and ‘Yingshuang’, respectively. The theanine contents in leaves and root of ‘Huangjinya’ were the highest, followed by ‘Anjibaicha’, and ‘Yingshuang’. The theanine contents in the leaves reduced as the leaf mature gradually, and in stem were the least. Seventeen genes encoding enzymes involved in theanine metabolism were identified from GenBank and our tea transcriptome database, including CsTS1, CsTS2, CsGS1, CsGS2, CsGOGAT-Fe, CsGOGAT-NAD(P)H, CsGDH1, CsGDH2, CsALT, CsSAMDC, CsADC, CsCuAO, CsPAO, CsNiR, CsNR, CsGGT1, and CsGGT3. The transcript profiles of those seventeen genes in the different tissues of three tea plant cultivars were analyzed comparatively. Among the different cultivars, the transcript levels of most selected genes in ‘Huangjinya’ were significantly higher than that in the ‘Anjibaicha’ and ‘Yingshuang’. Among the different tissues, the transcript levels of CsTS2, CsGS1, and CsGDH2 almost showed positive correlation with the theanine contents, while the other genes showed negative correlation with the theanine contents in most cases. The theanine contents showed correlations with related genes expression levels among cultivars and tissues of tea plant, and were determined by the integrated effect of the metabolic related genes.

No MeSH data available.


Three Camellia sinensis cultivars ‘Huangjinya’(HJY), ‘Anjibaicha’(AJBC), and ‘Yingshuang’(YS) (1) bud, (2) 1st leaf, (3) 2nd leaf, (4) 3rd leaf, (5) old leaf, (6) stem, (7) root.
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Figure 1: Three Camellia sinensis cultivars ‘Huangjinya’(HJY), ‘Anjibaicha’(AJBC), and ‘Yingshuang’(YS) (1) bud, (2) 1st leaf, (3) 2nd leaf, (4) 3rd leaf, (5) old leaf, (6) stem, (7) root.

Mentions: To obtain novel insight into L-theanine metabolism in tea plant, the genes encoding the enzymes involved in the theanine metabolism pathway are identified based on GenBank and our tea plant transcriptome (Wu et al., 2014). The expression patterns of seventeen metabolic-related genes and L-theanine content in the bud and 1st leaf, 2nd leaf, 3rd leaf, old leaf, stem, and root of the three selected tea cultivars were detected and analyzed by RP-HPLC method. The three C. sinensis cultivars, ‘Huangjinya’, ‘Anjibaicha’, and ‘Yingshuang’, were used in this experiment (Figure 1). Based on the obvious difference of geographic and climate characteristics in these three tea production areas, the morphology and physiology of the three tea plants were different. The theanine contents and the ability of adaptation to a single or multiple stresses were different among the three tea plant cultivars. The mainly characteristics of the three tea plant cultivars were listed in Table 1. This work is aimed to predict the relationship between structural genes and L-theanine content and the distribution at transcript level, and to provide reference for the further study of regulation mechanisms of theanine metabolism.


L -Theanine Content and Related Gene Expression: Novel Insights into Theanine Biosynthesis and Hydrolysis among Different Tea Plant ( Camellia sinensis L.) Tissues and Cultivars
Three Camellia sinensis cultivars ‘Huangjinya’(HJY), ‘Anjibaicha’(AJBC), and ‘Yingshuang’(YS) (1) bud, (2) 1st leaf, (3) 2nd leaf, (4) 3rd leaf, (5) old leaf, (6) stem, (7) root.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383724&req=5

Figure 1: Three Camellia sinensis cultivars ‘Huangjinya’(HJY), ‘Anjibaicha’(AJBC), and ‘Yingshuang’(YS) (1) bud, (2) 1st leaf, (3) 2nd leaf, (4) 3rd leaf, (5) old leaf, (6) stem, (7) root.
Mentions: To obtain novel insight into L-theanine metabolism in tea plant, the genes encoding the enzymes involved in the theanine metabolism pathway are identified based on GenBank and our tea plant transcriptome (Wu et al., 2014). The expression patterns of seventeen metabolic-related genes and L-theanine content in the bud and 1st leaf, 2nd leaf, 3rd leaf, old leaf, stem, and root of the three selected tea cultivars were detected and analyzed by RP-HPLC method. The three C. sinensis cultivars, ‘Huangjinya’, ‘Anjibaicha’, and ‘Yingshuang’, were used in this experiment (Figure 1). Based on the obvious difference of geographic and climate characteristics in these three tea production areas, the morphology and physiology of the three tea plants were different. The theanine contents and the ability of adaptation to a single or multiple stresses were different among the three tea plant cultivars. The mainly characteristics of the three tea plant cultivars were listed in Table 1. This work is aimed to predict the relationship between structural genes and L-theanine content and the distribution at transcript level, and to provide reference for the further study of regulation mechanisms of theanine metabolism.

View Article: PubMed Central - PubMed

ABSTRACT

L-Theanine content has tissues and cultivars specificity in tea plant (Camellia sinensis L.), the correlations of theanine metabolic related genes expression profiles with theanine contents were explored in this study. L-theanine contents in the bud and 1st leaf, 2nd leaf, 3rd leaf, old leaf, stem, and lateral root were determined by HPLC from three C. sinensis cultivars, namely ‘Huangjinya’, ‘Anjibaicha’, and ‘Yingshuang’, respectively. The theanine contents in leaves and root of ‘Huangjinya’ were the highest, followed by ‘Anjibaicha’, and ‘Yingshuang’. The theanine contents in the leaves reduced as the leaf mature gradually, and in stem were the least. Seventeen genes encoding enzymes involved in theanine metabolism were identified from GenBank and our tea transcriptome database, including CsTS1, CsTS2, CsGS1, CsGS2, CsGOGAT-Fe, CsGOGAT-NAD(P)H, CsGDH1, CsGDH2, CsALT, CsSAMDC, CsADC, CsCuAO, CsPAO, CsNiR, CsNR, CsGGT1, and CsGGT3. The transcript profiles of those seventeen genes in the different tissues of three tea plant cultivars were analyzed comparatively. Among the different cultivars, the transcript levels of most selected genes in ‘Huangjinya’ were significantly higher than that in the ‘Anjibaicha’ and ‘Yingshuang’. Among the different tissues, the transcript levels of CsTS2, CsGS1, and CsGDH2 almost showed positive correlation with the theanine contents, while the other genes showed negative correlation with the theanine contents in most cases. The theanine contents showed correlations with related genes expression levels among cultivars and tissues of tea plant, and were determined by the integrated effect of the metabolic related genes.

No MeSH data available.