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Intriguing Interaction of Bacteriophage-Host Association: An Understanding in the Era of Omics

View Article: PubMed Central - PubMed

ABSTRACT

Innovations in next-generation sequencing technology have introduced new avenues in microbial studies through “omics” approaches. This technology has considerably augmented the knowledge of the microbial world without isolation prior to their identification. With an enormous volume of bacterial “omics” data, considerable attempts have been recently invested to improve an insight into virosphere. The interplay between bacteriophages and their host has created a significant influence on the biogeochemical cycles, microbial diversity, and bacterial population regulation. This review highlights various concepts such as genomics, transcriptomics, proteomics, and metabolomics to infer the phylogenetic affiliation and function of bacteriophages and their impact on diverse microbial communities. Omics technologies illuminate the role of bacteriophage in an environment, the influences of phage proteins on the bacterial host and provide information about the genes important for interaction with bacteria. These investigations will reveal some of bio-molecules and biomarkers of the novel phage which demand to be unveiled.

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Related in: MedlinePlus

Understanding phage diversity, community interactions and chemical profiles using meta-omics approach. Genomics elucidates the phage diversity, abundance, probable functional features while transcriptomics gives an insight about the actively expressed genes in a community. Proteomics suggests the phage structural proteins, its functions and the proteins responsible for interaction between phage and host. Metabolomics advocates the metabolites produced by hosts in presence and absence of phage infection, alterations in regulation and metabolic profiles after infection. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; ESI-MS, electron spray ionization–mass spectroscopy; LC-MS, liquid chromatography–mass spectrometry; MALDI-MS, matrix-assisted laser ionization and deionization; NMR, nuclear magnetic resonance; NIMS, nanostructure initiator MS.
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Figure 4: Understanding phage diversity, community interactions and chemical profiles using meta-omics approach. Genomics elucidates the phage diversity, abundance, probable functional features while transcriptomics gives an insight about the actively expressed genes in a community. Proteomics suggests the phage structural proteins, its functions and the proteins responsible for interaction between phage and host. Metabolomics advocates the metabolites produced by hosts in presence and absence of phage infection, alterations in regulation and metabolic profiles after infection. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; ESI-MS, electron spray ionization–mass spectroscopy; LC-MS, liquid chromatography–mass spectrometry; MALDI-MS, matrix-assisted laser ionization and deionization; NMR, nuclear magnetic resonance; NIMS, nanostructure initiator MS.

Mentions: Metagenomics and SCG technologies can be strongly adapted to illustrate the exact identity and diversity of phages in an environment which can guide across the dark matter of viral ecosystem (Figure 4). Along with investigating the diversity of phages in an environment, to succumb with a coherent outline of their functional aspect in an ecosystem, it becomes imperative to deduce mechanisms underlying transcriptions of phages. Hence, transcriptomic studies provide knowledge about functions of active genes in given condition.


Intriguing Interaction of Bacteriophage-Host Association: An Understanding in the Era of Omics
Understanding phage diversity, community interactions and chemical profiles using meta-omics approach. Genomics elucidates the phage diversity, abundance, probable functional features while transcriptomics gives an insight about the actively expressed genes in a community. Proteomics suggests the phage structural proteins, its functions and the proteins responsible for interaction between phage and host. Metabolomics advocates the metabolites produced by hosts in presence and absence of phage infection, alterations in regulation and metabolic profiles after infection. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; ESI-MS, electron spray ionization–mass spectroscopy; LC-MS, liquid chromatography–mass spectrometry; MALDI-MS, matrix-assisted laser ionization and deionization; NMR, nuclear magnetic resonance; NIMS, nanostructure initiator MS.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383658&req=5

Figure 4: Understanding phage diversity, community interactions and chemical profiles using meta-omics approach. Genomics elucidates the phage diversity, abundance, probable functional features while transcriptomics gives an insight about the actively expressed genes in a community. Proteomics suggests the phage structural proteins, its functions and the proteins responsible for interaction between phage and host. Metabolomics advocates the metabolites produced by hosts in presence and absence of phage infection, alterations in regulation and metabolic profiles after infection. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; ESI-MS, electron spray ionization–mass spectroscopy; LC-MS, liquid chromatography–mass spectrometry; MALDI-MS, matrix-assisted laser ionization and deionization; NMR, nuclear magnetic resonance; NIMS, nanostructure initiator MS.
Mentions: Metagenomics and SCG technologies can be strongly adapted to illustrate the exact identity and diversity of phages in an environment which can guide across the dark matter of viral ecosystem (Figure 4). Along with investigating the diversity of phages in an environment, to succumb with a coherent outline of their functional aspect in an ecosystem, it becomes imperative to deduce mechanisms underlying transcriptions of phages. Hence, transcriptomic studies provide knowledge about functions of active genes in given condition.

View Article: PubMed Central - PubMed

ABSTRACT

Innovations in next-generation sequencing technology have introduced new avenues in microbial studies through “omics” approaches. This technology has considerably augmented the knowledge of the microbial world without isolation prior to their identification. With an enormous volume of bacterial “omics” data, considerable attempts have been recently invested to improve an insight into virosphere. The interplay between bacteriophages and their host has created a significant influence on the biogeochemical cycles, microbial diversity, and bacterial population regulation. This review highlights various concepts such as genomics, transcriptomics, proteomics, and metabolomics to infer the phylogenetic affiliation and function of bacteriophages and their impact on diverse microbial communities. Omics technologies illuminate the role of bacteriophage in an environment, the influences of phage proteins on the bacterial host and provide information about the genes important for interaction with bacteria. These investigations will reveal some of bio-molecules and biomarkers of the novel phage which demand to be unveiled.

No MeSH data available.


Related in: MedlinePlus