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Exploration of Elite Stilbene Synthase Alleles for Resveratrol Concentration in Wild Chinese Vitis spp. and Vitis Cultivars

View Article: PubMed Central - PubMed

ABSTRACT

Resveratrol contributes to a plant’s tolerance of various abiotic and biotic stresses and is highly beneficial to human health. A search for elite alleles affecting resveratrol production was undertaken to find useful grapevine germplasm resources. Resveratrol levels in both berry skins and leaves were determined in 95 grapevine accessions (including 50 wild Chinese grapevine accessions and 45 cultivars) during two consecutive years. Resveratrol contents were higher in berry skins than in leaves and in wild Chinese grapevines than in grapevine cultivars. Using genotyping data, 79 simple sequence repeat (SSR) markers linked to 44 stilbene synthase (STS) genes were detected in the 95 accessions, identifying 40 SSR markers with higher polymorphisms. Eight SSR marker loci, encompassing 19 alleles, were significantly associated with resveratrol content on (P < 0.001), and 5 SSR loci showed repeated associations. Locus Sh5 had four associations: three positive for allele 232 (including leaves in the 2 years) and one negative for allele 236 in four environments. Loci Sh9 and Sh56 for a total of 7 alleles exhibited positive effects in berry skins in the 2 years. In berry skins, locus Sh56 with positive effects was closely linked to VvSTS27, and locus Sh77 with negative effects to VvSTS17, importantly, the two candidate genes both were located on Chromosome 16. The SSR marker loci and candidate genes identified in this study will provide a useful basis for future molecular breeding for increased production of natural resveratrol and its derivatives.

No MeSH data available.


Related in: MedlinePlus

Distribution of Linkage disequilibrium (LD) among 40 SSR loci in two groups of 95 grapevine accessions. SSR markers are organized in linkage groups marked along the X- and Y-axis; each pixel above the diagonal indicates the D′value of the corresponding marker pair as shown in the color code at the upper right, while each pixel below the diagonal indicates the p-value size of the testing LD of the corresponding marker pairs as shown in the color code at the lower right. (A) Distribution of LD in 95 grapevine accessions. (B) 50 wild Chinese accessions. (C) 45 grapevine cultivars.
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Figure 4: Distribution of Linkage disequilibrium (LD) among 40 SSR loci in two groups of 95 grapevine accessions. SSR markers are organized in linkage groups marked along the X- and Y-axis; each pixel above the diagonal indicates the D′value of the corresponding marker pair as shown in the color code at the upper right, while each pixel below the diagonal indicates the p-value size of the testing LD of the corresponding marker pairs as shown in the color code at the lower right. (A) Distribution of LD in 95 grapevine accessions. (B) 50 wild Chinese accessions. (C) 45 grapevine cultivars.

Mentions: Linkage disequilibrium (LD) among genes was the basis of the association analysis. Distribution of LD among the 40 SSR loci in the two groups (according to Figure 2) was shown as Figure 4A. Loci with high LD values (D′ > 0.7; upper right corner) were Sh13, Sh16, Sh22, Sh31, Sh37, Sh68, and Sh78.The LD among the wild Chinese grapevines (Figure 4B) was significantly higher than those of the grapevine cultivars (Figure 4C, including table grapes and wine grapes). The mean frequency distribution of the D′ value (P < 0.001) was 0.5329 for all experimental samples (Table 2A), 0.6046 for the V. vinifera cultivars, and 0.7037 for the wild Chinese accessions (Table 2B). The higher D′ in the wild population indicates more variation. In addition, the number of LD loci among the grapevine cultivars was fewer than in the wild Chinese accessions (Table 2B).


Exploration of Elite Stilbene Synthase Alleles for Resveratrol Concentration in Wild Chinese Vitis spp. and Vitis Cultivars
Distribution of Linkage disequilibrium (LD) among 40 SSR loci in two groups of 95 grapevine accessions. SSR markers are organized in linkage groups marked along the X- and Y-axis; each pixel above the diagonal indicates the D′value of the corresponding marker pair as shown in the color code at the upper right, while each pixel below the diagonal indicates the p-value size of the testing LD of the corresponding marker pairs as shown in the color code at the lower right. (A) Distribution of LD in 95 grapevine accessions. (B) 50 wild Chinese accessions. (C) 45 grapevine cultivars.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383651&req=5

Figure 4: Distribution of Linkage disequilibrium (LD) among 40 SSR loci in two groups of 95 grapevine accessions. SSR markers are organized in linkage groups marked along the X- and Y-axis; each pixel above the diagonal indicates the D′value of the corresponding marker pair as shown in the color code at the upper right, while each pixel below the diagonal indicates the p-value size of the testing LD of the corresponding marker pairs as shown in the color code at the lower right. (A) Distribution of LD in 95 grapevine accessions. (B) 50 wild Chinese accessions. (C) 45 grapevine cultivars.
Mentions: Linkage disequilibrium (LD) among genes was the basis of the association analysis. Distribution of LD among the 40 SSR loci in the two groups (according to Figure 2) was shown as Figure 4A. Loci with high LD values (D′ > 0.7; upper right corner) were Sh13, Sh16, Sh22, Sh31, Sh37, Sh68, and Sh78.The LD among the wild Chinese grapevines (Figure 4B) was significantly higher than those of the grapevine cultivars (Figure 4C, including table grapes and wine grapes). The mean frequency distribution of the D′ value (P < 0.001) was 0.5329 for all experimental samples (Table 2A), 0.6046 for the V. vinifera cultivars, and 0.7037 for the wild Chinese accessions (Table 2B). The higher D′ in the wild population indicates more variation. In addition, the number of LD loci among the grapevine cultivars was fewer than in the wild Chinese accessions (Table 2B).

View Article: PubMed Central - PubMed

ABSTRACT

Resveratrol contributes to a plant&rsquo;s tolerance of various abiotic and biotic stresses and is highly beneficial to human health. A search for elite alleles affecting resveratrol production was undertaken to find useful grapevine germplasm resources. Resveratrol levels in both berry skins and leaves were determined in 95 grapevine accessions (including 50 wild Chinese grapevine accessions and 45 cultivars) during two consecutive years. Resveratrol contents were higher in berry skins than in leaves and in wild Chinese grapevines than in grapevine cultivars. Using genotyping data, 79 simple sequence repeat (SSR) markers linked to 44 stilbene synthase (STS) genes were detected in the 95 accessions, identifying 40 SSR markers with higher polymorphisms. Eight SSR marker loci, encompassing 19 alleles, were significantly associated with resveratrol content on (P &lt; 0.001), and 5 SSR loci showed repeated associations. Locus Sh5 had four associations: three positive for allele 232 (including leaves in the 2 years) and one negative for allele 236 in four environments. Loci Sh9 and Sh56 for a total of 7 alleles exhibited positive effects in berry skins in the 2 years. In berry skins, locus Sh56 with positive effects was closely linked to VvSTS27, and locus Sh77 with negative effects to VvSTS17, importantly, the two candidate genes both were located on Chromosome 16. The SSR marker loci and candidate genes identified in this study will provide a useful basis for future molecular breeding for increased production of natural resveratrol and its derivatives.

No MeSH data available.


Related in: MedlinePlus