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New methods for isolation of keratolytic bacteria inducing intractable hoof wall cavity ( Gidoh ) in a horse; double screening procedures of the horn powder agar-translucency test and horn zymography

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ABSTRACT

To establish a new system to isolate keratolytic bacteria from the hoof wall cavity (Gidoh) of a racehorse, we invented the horn powder agar-translucency(HoPAT) test and horn zymography (HZ). Using routine bacteriological techniques and these methods, we isolated five strains of keratolytic soil bacteria, whichwere then identified by means of 16S ribosomal RNA (rRNA) gene sequencing analysis. The findings from the study on the horse suggested that Brevibacteriumluteolum played the main role in the local fragility of the hoof, eventually forming a Gidoh in coordination with four other strains of keratolytic bacteria.The double screening procedures of the HoPAT test and HZ were useful and easy techniques for isolating the keratolytic bacteria from the horn lesions.

No MeSH data available.


Related in: MedlinePlus

One of the culture results from the horn powder agar-translucency test: The strain in (corresponding to No. 2) appeared as a yellowish colony and presented a stronger and wider translucency characteristic around the colony on the horn powderagar than other strains. The colony in  on the right side of No. 2 showed weak translucency,whereas  had no translucency characteristic.
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fig_003: One of the culture results from the horn powder agar-translucency test: The strain in (corresponding to No. 2) appeared as a yellowish colony and presented a stronger and wider translucency characteristic around the colony on the horn powderagar than other strains. The colony in on the right side of No. 2 showed weak translucency,whereas had no translucency characteristic.

Mentions: HoPAT; Horn powder agar translucency.


New methods for isolation of keratolytic bacteria inducing intractable hoof wall cavity ( Gidoh ) in a horse; double screening procedures of the horn powder agar-translucency test and horn zymography
One of the culture results from the horn powder agar-translucency test: The strain in (corresponding to No. 2) appeared as a yellowish colony and presented a stronger and wider translucency characteristic around the colony on the horn powderagar than other strains. The colony in  on the right side of No. 2 showed weak translucency,whereas  had no translucency characteristic.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383627&req=5

fig_003: One of the culture results from the horn powder agar-translucency test: The strain in (corresponding to No. 2) appeared as a yellowish colony and presented a stronger and wider translucency characteristic around the colony on the horn powderagar than other strains. The colony in on the right side of No. 2 showed weak translucency,whereas had no translucency characteristic.
Mentions: HoPAT; Horn powder agar translucency.

View Article: PubMed Central - PubMed

ABSTRACT

To establish a new system to isolate keratolytic bacteria from the hoof wall cavity (Gidoh) of a racehorse, we invented the horn powder agar-translucency(HoPAT) test and horn zymography (HZ). Using routine bacteriological techniques and these methods, we isolated five strains of keratolytic soil bacteria, whichwere then identified by means of 16S ribosomal RNA (rRNA) gene sequencing analysis. The findings from the study on the horse suggested that Brevibacteriumluteolum played the main role in the local fragility of the hoof, eventually forming a Gidoh in coordination with four other strains of keratolytic bacteria.The double screening procedures of the HoPAT test and HZ were useful and easy techniques for isolating the keratolytic bacteria from the horn lesions.

No MeSH data available.


Related in: MedlinePlus