Limits...
New methods for isolation of keratolytic bacteria inducing intractable hoof wall cavity ( Gidoh ) in a horse; double screening procedures of the horn powder agar-translucency test and horn zymography

View Article: PubMed Central - PubMed

ABSTRACT

To establish a new system to isolate keratolytic bacteria from the hoof wall cavity (Gidoh) of a racehorse, we invented the horn powder agar-translucency(HoPAT) test and horn zymography (HZ). Using routine bacteriological techniques and these methods, we isolated five strains of keratolytic soil bacteria, whichwere then identified by means of 16S ribosomal RNA (rRNA) gene sequencing analysis. The findings from the study on the horse suggested that Brevibacteriumluteolum played the main role in the local fragility of the hoof, eventually forming a Gidoh in coordination with four other strains of keratolytic bacteria.The double screening procedures of the HoPAT test and HZ were useful and easy techniques for isolating the keratolytic bacteria from the horn lesions.

No MeSH data available.


A large number of Gram-positive bacteria were attached to the border of the hoof wall cavity (a) and invaded and proliferated inside the horn tissues nearthe cavity (b). All bacteria were observed within 300 µm from the edge of the lesion in the depth direction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5383627&req=5

fig_002: A large number of Gram-positive bacteria were attached to the border of the hoof wall cavity (a) and invaded and proliferated inside the horn tissues nearthe cavity (b). All bacteria were observed within 300 µm from the edge of the lesion in the depth direction.

Mentions: Within the histopathological findings, a large number of Gram-positive cocci and bacilli and a few Gram-negative cocci attached to the borders of the hoof wallcavities were identified (Fig. 2aFig. 2.


New methods for isolation of keratolytic bacteria inducing intractable hoof wall cavity ( Gidoh ) in a horse; double screening procedures of the horn powder agar-translucency test and horn zymography
A large number of Gram-positive bacteria were attached to the border of the hoof wall cavity (a) and invaded and proliferated inside the horn tissues nearthe cavity (b). All bacteria were observed within 300 µm from the edge of the lesion in the depth direction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383627&req=5

fig_002: A large number of Gram-positive bacteria were attached to the border of the hoof wall cavity (a) and invaded and proliferated inside the horn tissues nearthe cavity (b). All bacteria were observed within 300 µm from the edge of the lesion in the depth direction.
Mentions: Within the histopathological findings, a large number of Gram-positive cocci and bacilli and a few Gram-negative cocci attached to the borders of the hoof wallcavities were identified (Fig. 2aFig. 2.

View Article: PubMed Central - PubMed

ABSTRACT

To establish a new system to isolate keratolytic bacteria from the hoof wall cavity (Gidoh) of a racehorse, we invented the horn powder agar-translucency(HoPAT) test and horn zymography (HZ). Using routine bacteriological techniques and these methods, we isolated five strains of keratolytic soil bacteria, whichwere then identified by means of 16S ribosomal RNA (rRNA) gene sequencing analysis. The findings from the study on the horse suggested that Brevibacteriumluteolum played the main role in the local fragility of the hoof, eventually forming a Gidoh in coordination with four other strains of keratolytic bacteria.The double screening procedures of the HoPAT test and HZ were useful and easy techniques for isolating the keratolytic bacteria from the horn lesions.

No MeSH data available.