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New methods for isolation of keratolytic bacteria inducing intractable hoof wall cavity ( Gidoh ) in a horse; double screening procedures of the horn powder agar-translucency test and horn zymography

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ABSTRACT

To establish a new system to isolate keratolytic bacteria from the hoof wall cavity (Gidoh) of a racehorse, we invented the horn powder agar-translucency(HoPAT) test and horn zymography (HZ). Using routine bacteriological techniques and these methods, we isolated five strains of keratolytic soil bacteria, whichwere then identified by means of 16S ribosomal RNA (rRNA) gene sequencing analysis. The findings from the study on the horse suggested that Brevibacteriumluteolum played the main role in the local fragility of the hoof, eventually forming a Gidoh in coordination with four other strains of keratolytic bacteria.The double screening procedures of the HoPAT test and HZ were useful and easy techniques for isolating the keratolytic bacteria from the horn lesions.

No MeSH data available.


A fragile area of the horn was observed at the toe of the left foot. A progressive fine linear cavity ran through the middle of the fragile lesion. Inthis picture, a cross-sectional surface of the cavity shows a punctate hole between the stratum medium and the stratuminternum.
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fig_001: A fragile area of the horn was observed at the toe of the left foot. A progressive fine linear cavity ran through the middle of the fragile lesion. Inthis picture, a cross-sectional surface of the cavity shows a punctate hole between the stratum medium and the stratuminternum.

Mentions: The studied horse (thoroughbred, male, six years old) was retired from racing due to the recurrent white line disease-type Gidoh [16, 20] manifesting on the forefeet with no response to regular shoeing, correctivefarriery, and local debridement. At the first examination after retirement, we identified an obsolete dry cavity in the wall of the right forefoot and a freshmoist cavity in the wall of the left forefoot. The latter cavity showed fine branching lines that ran between the stratum medium and thestratum internum of the wall from the bearing surface of the white line to 5 cm above the bearing border (Fig. 1Fig. 1.


New methods for isolation of keratolytic bacteria inducing intractable hoof wall cavity ( Gidoh ) in a horse; double screening procedures of the horn powder agar-translucency test and horn zymography
A fragile area of the horn was observed at the toe of the left foot. A progressive fine linear cavity ran through the middle of the fragile lesion. Inthis picture, a cross-sectional surface of the cavity shows a punctate hole between the stratum medium and the stratuminternum.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383627&req=5

fig_001: A fragile area of the horn was observed at the toe of the left foot. A progressive fine linear cavity ran through the middle of the fragile lesion. Inthis picture, a cross-sectional surface of the cavity shows a punctate hole between the stratum medium and the stratuminternum.
Mentions: The studied horse (thoroughbred, male, six years old) was retired from racing due to the recurrent white line disease-type Gidoh [16, 20] manifesting on the forefeet with no response to regular shoeing, correctivefarriery, and local debridement. At the first examination after retirement, we identified an obsolete dry cavity in the wall of the right forefoot and a freshmoist cavity in the wall of the left forefoot. The latter cavity showed fine branching lines that ran between the stratum medium and thestratum internum of the wall from the bearing surface of the white line to 5 cm above the bearing border (Fig. 1Fig. 1.

View Article: PubMed Central - PubMed

ABSTRACT

To establish a new system to isolate keratolytic bacteria from the hoof wall cavity (Gidoh) of a racehorse, we invented the horn powder agar-translucency(HoPAT) test and horn zymography (HZ). Using routine bacteriological techniques and these methods, we isolated five strains of keratolytic soil bacteria, whichwere then identified by means of 16S ribosomal RNA (rRNA) gene sequencing analysis. The findings from the study on the horse suggested that Brevibacteriumluteolum played the main role in the local fragility of the hoof, eventually forming a Gidoh in coordination with four other strains of keratolytic bacteria.The double screening procedures of the HoPAT test and HZ were useful and easy techniques for isolating the keratolytic bacteria from the horn lesions.

No MeSH data available.