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Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains

View Article: PubMed Central - PubMed

ABSTRACT

Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.

Doi:: http://dx.doi.org/10.7554/eLife.25114.001

No MeSH data available.


FLS2 still undergoes cluster formation in serk mutant backgrounds.DOI:http://dx.doi.org/10.7554/eLife.25114.026
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fig13: FLS2 still undergoes cluster formation in serk mutant backgrounds.DOI:http://dx.doi.org/10.7554/eLife.25114.026

Mentions: As mentioned earlier (see response to general editorial comments), our included preliminary data (Author response image 1) indicate the co-localisation of BAK1 with BRI1 and FLS2 in certain PM nanodomains. As part of another submitted manuscript (Hutten et al., under revision with PLoS One; co-last authors: Johannes Hohlbein & Jan Willem Borst; co-auther: CA Bücherl), we show that absence of SERK1 and BAK1 does not abolish cluster formation for BRI1. Further, we show here preliminary data indicating a similar situation for FLS2 (see Author response image 4).10.7554/eLife.25114.026Author response image 4.FLS2 still undergoes cluster formation in serk mutant backgrounds.


Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains
FLS2 still undergoes cluster formation in serk mutant backgrounds.DOI:http://dx.doi.org/10.7554/eLife.25114.026
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383397&req=5

fig13: FLS2 still undergoes cluster formation in serk mutant backgrounds.DOI:http://dx.doi.org/10.7554/eLife.25114.026
Mentions: As mentioned earlier (see response to general editorial comments), our included preliminary data (Author response image 1) indicate the co-localisation of BAK1 with BRI1 and FLS2 in certain PM nanodomains. As part of another submitted manuscript (Hutten et al., under revision with PLoS One; co-last authors: Johannes Hohlbein & Jan Willem Borst; co-auther: CA Bücherl), we show that absence of SERK1 and BAK1 does not abolish cluster formation for BRI1. Further, we show here preliminary data indicating a similar situation for FLS2 (see Author response image 4).10.7554/eLife.25114.026Author response image 4.FLS2 still undergoes cluster formation in serk mutant backgrounds.

View Article: PubMed Central - PubMed

ABSTRACT

Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.

Doi:: http://dx.doi.org/10.7554/eLife.25114.001

No MeSH data available.