Limits...
Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains

View Article: PubMed Central - PubMed

ABSTRACT

Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.

Doi:: http://dx.doi.org/10.7554/eLife.25114.001

No MeSH data available.


Actin depolymerisation does not abolish FLS2 cluster formation.DOI:http://dx.doi.org/10.7554/eLife.25114.025
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5383397&req=5

fig12: Actin depolymerisation does not abolish FLS2 cluster formation.DOI:http://dx.doi.org/10.7554/eLife.25114.025

Mentions: The physiological characterisation of rem mutants is ongoing. It is however worthwhile to note that no significant phenotype has ever been reported so far for any single rem mutant in Arabidopsis, and thus that the generation of multiple mutants may be required. Similarly, studies on the interplay between cytoskeleton and receptor clusters are in progress. Preliminary data indicate that cluster formation is not abolished upon actin depolymerisation, for example; however, the influence on cluster dynamics is still unclear (see Author response image 3).10.7554/eLife.25114.025Author response image 3.Actin depolymerisation does not abolish FLS2 cluster formation.


Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains
Actin depolymerisation does not abolish FLS2 cluster formation.DOI:http://dx.doi.org/10.7554/eLife.25114.025
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383397&req=5

fig12: Actin depolymerisation does not abolish FLS2 cluster formation.DOI:http://dx.doi.org/10.7554/eLife.25114.025
Mentions: The physiological characterisation of rem mutants is ongoing. It is however worthwhile to note that no significant phenotype has ever been reported so far for any single rem mutant in Arabidopsis, and thus that the generation of multiple mutants may be required. Similarly, studies on the interplay between cytoskeleton and receptor clusters are in progress. Preliminary data indicate that cluster formation is not abolished upon actin depolymerisation, for example; however, the influence on cluster dynamics is still unclear (see Author response image 3).10.7554/eLife.25114.025Author response image 3.Actin depolymerisation does not abolish FLS2 cluster formation.

View Article: PubMed Central - PubMed

ABSTRACT

Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.

Doi:: http://dx.doi.org/10.7554/eLife.25114.001

No MeSH data available.