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Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains

View Article: PubMed Central - PubMed

ABSTRACT

Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.

Doi:: http://dx.doi.org/10.7554/eLife.25114.001

No MeSH data available.


Related in: MedlinePlus

FLS2-GFP forms receptor clusters in fls2 mutant background.The images show the plasma membrane localisation of pFLS2::FLS2-GFP stably transformed in the fls2 mutant or Columbia wild type background, respectively. The images were acquired 5 days post germination using CLSM and the scale bars represents a distance of 5 µm.DOI:http://dx.doi.org/10.7554/eLife.25114.024
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fig11: FLS2-GFP forms receptor clusters in fls2 mutant background.The images show the plasma membrane localisation of pFLS2::FLS2-GFP stably transformed in the fls2 mutant or Columbia wild type background, respectively. The images were acquired 5 days post germination using CLSM and the scale bars represents a distance of 5 µm.DOI:http://dx.doi.org/10.7554/eLife.25114.024

Mentions: Regarding the presence of endogenous receptors on the analysis, we do not think that a withdrawal of endogenous populations would change the conclusions of our observations. Preliminary data indicate no major differences for FLS2-GFP expressed under the FLS2 promoter in either wild-type Col-0 or fls2 mutant background (see Author response image 2). Though, the presence of endogenous receptors may lead to reduced fluorescence in certain clusters, which would not affect the present conclusions.10.7554/eLife.25114.024Author response image 2.FLS2-GFP forms receptor clusters in fls2 mutant background.


Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains
FLS2-GFP forms receptor clusters in fls2 mutant background.The images show the plasma membrane localisation of pFLS2::FLS2-GFP stably transformed in the fls2 mutant or Columbia wild type background, respectively. The images were acquired 5 days post germination using CLSM and the scale bars represents a distance of 5 µm.DOI:http://dx.doi.org/10.7554/eLife.25114.024
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5383397&req=5

fig11: FLS2-GFP forms receptor clusters in fls2 mutant background.The images show the plasma membrane localisation of pFLS2::FLS2-GFP stably transformed in the fls2 mutant or Columbia wild type background, respectively. The images were acquired 5 days post germination using CLSM and the scale bars represents a distance of 5 µm.DOI:http://dx.doi.org/10.7554/eLife.25114.024
Mentions: Regarding the presence of endogenous receptors on the analysis, we do not think that a withdrawal of endogenous populations would change the conclusions of our observations. Preliminary data indicate no major differences for FLS2-GFP expressed under the FLS2 promoter in either wild-type Col-0 or fls2 mutant background (see Author response image 2). Though, the presence of endogenous receptors may lead to reduced fluorescence in certain clusters, which would not affect the present conclusions.10.7554/eLife.25114.024Author response image 2.FLS2-GFP forms receptor clusters in fls2 mutant background.

View Article: PubMed Central - PubMed

ABSTRACT

Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.

Doi:: http://dx.doi.org/10.7554/eLife.25114.001

No MeSH data available.


Related in: MedlinePlus