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Serum Th1 and Th17 related cytokines and autoantibodies in patients with Posner-Schlossman syndrome

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ABSTRACT

Posner-Schlossman syndrome (PSS) shares some clinical features with uveitis and open angle glaucoma. Cytokines and autoantibodies have been associated with uveitis and open angle glaucoma. However, the role of serum cytokines and autoantibodies in the pathogenesis of PSS remains unknown. This study aimed to evaluate the associations of type 1 T helper (Th1) and Th17 related cytokines and autoantibodies with PSS. Peripheral blood serum samples were collected from 81 patients with PSS and 97 gender- and age-matched healthy blood donors. Th1 and Th17 related cytokines, including interleukin-1β (IL-1β), IL-12, tumor necrosis factor-α (TNF-α), interferon- γ (IFN-γ), IL-6 and IL-17, and glucose-6-phosphate isomerase (GPI) were determined by double antibody sandwich ELISA. Anti-nuclear antibody (ANA), anti-keratin antibody (AKA) and anti-neutrophil cytoplasmic antibody (ANCA) were detected by indirect immunofluorescence assay. Anti-cardiolipin antibody (ACA)-IgG, ACA-IgM, ACA-IgA, anti-double stranded DNA (anti-dsDNA) and anti-cyclic citrullinated peptide antibody (anti-CCP) were detected by indirect ELISA. Serum levels of IL-1β, IL-12 and IL-6 in PSS patients were significantly lower than those in controls (P < 0.003), and these associations survived the Bonferroni correction (Pc < 0.018). There was no significant difference in serum levels of TNF-α, IFN-γ and IL-17 between the PSS and control groups (Pc > 0.12). Positive rate of serum anti-dsDNA in PSS patients was significantly higher than that in the control group (P = 0.002, Pc = 0.018), while positive rates of serum ANA, AKA, ANCA, ACA-IgG, ACA-IgM, ACA-IgA, GPI and anti-CCP in the PSS group were not significantly different from those in the control group (Pc > 0.09). These results suggest that anti-dsDNA may contribute to the pathogenesis of PSS, while Th1 and Th17 related cytokines and other autoantibodies may not be major contributors to PSS.

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Diagram of the network on interactions among the Th1 and Th17 related cytokines, anti-double stranded DNA (anti-dsDNA) and Glucose-6-Phosphate Isomerase (GPI).Abbreviations: IL, interleukin; TNF-α, tumor necrosis factor-α; IFN-γ, interferon- γ. Arrows indicate stimulatory effect, bar-headed line indicates inhibitory effect and dashed arrows indicate indirect effect.
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pone.0175519.g001: Diagram of the network on interactions among the Th1 and Th17 related cytokines, anti-double stranded DNA (anti-dsDNA) and Glucose-6-Phosphate Isomerase (GPI).Abbreviations: IL, interleukin; TNF-α, tumor necrosis factor-α; IFN-γ, interferon- γ. Arrows indicate stimulatory effect, bar-headed line indicates inhibitory effect and dashed arrows indicate indirect effect.

Mentions: IL-1 is an early inflammatory cytokine with the ability to regulate immune and inflammatory responses in vivo. Since IL-1α is mainly present in the cell and IL-1β is mainly present in the body fluid, the biological effects of IL-1 are mainly mediated by IL-1β [18]. Fleisher et al. reported that IL-1β could stimulate the production of IL-6 (Fig 1), leading to intraocular inflammation in uveitis [19]. In the present study, we found that serum IL-1β levels in PSS patients were significantly lower than those in normal controls (Table 3). Intriguingly, Li et al. also reported that aqueous IL-1β levels in patients with PSS were lower than those in cataract controls although the difference did not reach statistical significance possibly due to the small sample sizes [5]. These findings indicate that IL-1β might not be the main cytokine responsible for the inflammatory response in PSS.


Serum Th1 and Th17 related cytokines and autoantibodies in patients with Posner-Schlossman syndrome
Diagram of the network on interactions among the Th1 and Th17 related cytokines, anti-double stranded DNA (anti-dsDNA) and Glucose-6-Phosphate Isomerase (GPI).Abbreviations: IL, interleukin; TNF-α, tumor necrosis factor-α; IFN-γ, interferon- γ. Arrows indicate stimulatory effect, bar-headed line indicates inhibitory effect and dashed arrows indicate indirect effect.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5383301&req=5

pone.0175519.g001: Diagram of the network on interactions among the Th1 and Th17 related cytokines, anti-double stranded DNA (anti-dsDNA) and Glucose-6-Phosphate Isomerase (GPI).Abbreviations: IL, interleukin; TNF-α, tumor necrosis factor-α; IFN-γ, interferon- γ. Arrows indicate stimulatory effect, bar-headed line indicates inhibitory effect and dashed arrows indicate indirect effect.
Mentions: IL-1 is an early inflammatory cytokine with the ability to regulate immune and inflammatory responses in vivo. Since IL-1α is mainly present in the cell and IL-1β is mainly present in the body fluid, the biological effects of IL-1 are mainly mediated by IL-1β [18]. Fleisher et al. reported that IL-1β could stimulate the production of IL-6 (Fig 1), leading to intraocular inflammation in uveitis [19]. In the present study, we found that serum IL-1β levels in PSS patients were significantly lower than those in normal controls (Table 3). Intriguingly, Li et al. also reported that aqueous IL-1β levels in patients with PSS were lower than those in cataract controls although the difference did not reach statistical significance possibly due to the small sample sizes [5]. These findings indicate that IL-1β might not be the main cytokine responsible for the inflammatory response in PSS.

View Article: PubMed Central - PubMed

ABSTRACT

Posner-Schlossman syndrome (PSS) shares some clinical features with uveitis and open angle glaucoma. Cytokines and autoantibodies have been associated with uveitis and open angle glaucoma. However, the role of serum cytokines and autoantibodies in the pathogenesis of PSS remains unknown. This study aimed to evaluate the associations of type 1 T helper (Th1) and Th17 related cytokines and autoantibodies with PSS. Peripheral blood serum samples were collected from 81 patients with PSS and 97 gender- and age-matched healthy blood donors. Th1 and Th17 related cytokines, including interleukin-1β (IL-1β), IL-12, tumor necrosis factor-α (TNF-α), interferon- γ (IFN-γ), IL-6 and IL-17, and glucose-6-phosphate isomerase (GPI) were determined by double antibody sandwich ELISA. Anti-nuclear antibody (ANA), anti-keratin antibody (AKA) and anti-neutrophil cytoplasmic antibody (ANCA) were detected by indirect immunofluorescence assay. Anti-cardiolipin antibody (ACA)-IgG, ACA-IgM, ACA-IgA, anti-double stranded DNA (anti-dsDNA) and anti-cyclic citrullinated peptide antibody (anti-CCP) were detected by indirect ELISA. Serum levels of IL-1β, IL-12 and IL-6 in PSS patients were significantly lower than those in controls (P < 0.003), and these associations survived the Bonferroni correction (Pc < 0.018). There was no significant difference in serum levels of TNF-α, IFN-γ and IL-17 between the PSS and control groups (Pc > 0.12). Positive rate of serum anti-dsDNA in PSS patients was significantly higher than that in the control group (P = 0.002, Pc = 0.018), while positive rates of serum ANA, AKA, ANCA, ACA-IgG, ACA-IgM, ACA-IgA, GPI and anti-CCP in the PSS group were not significantly different from those in the control group (Pc > 0.09). These results suggest that anti-dsDNA may contribute to the pathogenesis of PSS, while Th1 and Th17 related cytokines and other autoantibodies may not be major contributors to PSS.

No MeSH data available.


Related in: MedlinePlus