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Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep

View Article: PubMed Central - PubMed

ABSTRACT

Follistatin (FST) is involved in hair follicle morphogenesis. However, its effects on hair traits are not clear. This study was designed to investigate the effects of FST gene single nucleotide polymorphisms (SNP) on wool quality traits in Chinese Merino sheep (Junken Type). We performed gene expression analysis, SNP detection, and association analysis of FST gene with sheep wool quality traits. The real-time RT-PCR analysis showed that FST gene was differentially expressed in adult skin between Chinese Merino sheep (Junken Type) and Suffolk sheep. Immunostaining showed that FST was localized in inner root sheath (IRS) and matrix of hair follicle (HF) in both SF and Suffolk sheep. Sequencing analysis identified a total of seven SNPs (termed SNPs 1–7) in the FST gene in Chinese Merino sheep (Junken Type). Association analysis showed that SNP2 (Chr 16. 25,633,662 G>A) was significantly associated with average wool fiber diameter, wool fineness SD, and wool crimp (P < 0.05). SNP4 (Chr 16. 25,633,569 C>T) was significantly associated with wool fineness SD and CV of fiber diameter (P < 0.05). Similarly, the haplotypes derived from these seven identified SNPs were also significantly associated with average wool fiber diameter, wool fineness SD, CV of fiber diameter, and wool crimp (P < 0.05). Our results suggest that FST influences wool quality traits and its SNPs 2 and 4 might be useful markers for marker-assisted selection and sheep breeding.

No MeSH data available.


Ovine FST gene expression.(A) The average wool fiber diameter of SF (n = 3) and Suffolk (n = 3) sheep. In a row with different superscripts differ significantly (P < 0.05). (B) The FST gene expression in various tissues of the SF sheep (n = 3). The housekeeping gene GAPDH was used as an internal control for real-time RT-PCR analysis. In a row with different superscripts differ significantly (P < 0.05). (C) The FST gene expression levels in the body side skin between SF (n = 8) and Suffolk (n = 8). The housekeeping gene GAPDH was used as an internal control for real-time RT-PCR analysis. In a row with different superscripts differ significantly (P < 0.05). (D) Immunohistochemical localization of FST in SF sheep skin (n = 3). (E) Immunohistochemical localization of FST in Suffolk sheep skin (n = 3). The inner root sheath, arrowhead; matrix, arrow.
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pone.0174868.g001: Ovine FST gene expression.(A) The average wool fiber diameter of SF (n = 3) and Suffolk (n = 3) sheep. In a row with different superscripts differ significantly (P < 0.05). (B) The FST gene expression in various tissues of the SF sheep (n = 3). The housekeeping gene GAPDH was used as an internal control for real-time RT-PCR analysis. In a row with different superscripts differ significantly (P < 0.05). (C) The FST gene expression levels in the body side skin between SF (n = 8) and Suffolk (n = 8). The housekeeping gene GAPDH was used as an internal control for real-time RT-PCR analysis. In a row with different superscripts differ significantly (P < 0.05). (D) Immunohistochemical localization of FST in SF sheep skin (n = 3). (E) Immunohistochemical localization of FST in Suffolk sheep skin (n = 3). The inner root sheath, arrowhead; matrix, arrow.

Mentions: Merino and Suffolk are two different sheep breeds. There is a dramatic difference in average wool fiber diameter between the two sheep breeds [20]. Consistent with the known wool quality difference between Merino and Suffolk, our wool fiber measurement showed that the superfine wool strain (SF) Merino had lower average wool fiber diameter than Suffolk sheep (SF sheep: 17.58 μm; Suffolk: 30.92 μm; P = 0.0009; Fig 1A). To obtain the clue for understanding the effects of FST gene on sheep wool quality traits, we performed the tissue expression analysis of FST gene in SF sheep and Suffolk sheep by real-time RT-PCR. The results showed that FST gene was ubiquitously expressed in the eleven tested adult tissues of SF sheep (Fig 1B), and comparatively highly expressed in the body side skin and ovary (Fig 1B). Skin expression comparison revealed that FST gene expression was 4.15-fold lower in SF sheep than in Suffolk sheep (P < 0.05; Fig 1C). Correlation analysis showed that skin FST gene expression was positively significantly correlated to the average wool fiber diameter (Pearson’s r = 0.987, P < 0.05), suggesting that FST gene expression variation may contribute to the variation of wool quality traits between SF sheep and Suffolk sheep. Immunohistochemical localization showed that FST was localized in inner root sheath (IRS) and matrix of hair follicle (HF) in both SF and Suffolk sheep (Fig 1D and 1E).


Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep
Ovine FST gene expression.(A) The average wool fiber diameter of SF (n = 3) and Suffolk (n = 3) sheep. In a row with different superscripts differ significantly (P < 0.05). (B) The FST gene expression in various tissues of the SF sheep (n = 3). The housekeeping gene GAPDH was used as an internal control for real-time RT-PCR analysis. In a row with different superscripts differ significantly (P < 0.05). (C) The FST gene expression levels in the body side skin between SF (n = 8) and Suffolk (n = 8). The housekeeping gene GAPDH was used as an internal control for real-time RT-PCR analysis. In a row with different superscripts differ significantly (P < 0.05). (D) Immunohistochemical localization of FST in SF sheep skin (n = 3). (E) Immunohistochemical localization of FST in Suffolk sheep skin (n = 3). The inner root sheath, arrowhead; matrix, arrow.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5383234&req=5

pone.0174868.g001: Ovine FST gene expression.(A) The average wool fiber diameter of SF (n = 3) and Suffolk (n = 3) sheep. In a row with different superscripts differ significantly (P < 0.05). (B) The FST gene expression in various tissues of the SF sheep (n = 3). The housekeeping gene GAPDH was used as an internal control for real-time RT-PCR analysis. In a row with different superscripts differ significantly (P < 0.05). (C) The FST gene expression levels in the body side skin between SF (n = 8) and Suffolk (n = 8). The housekeeping gene GAPDH was used as an internal control for real-time RT-PCR analysis. In a row with different superscripts differ significantly (P < 0.05). (D) Immunohistochemical localization of FST in SF sheep skin (n = 3). (E) Immunohistochemical localization of FST in Suffolk sheep skin (n = 3). The inner root sheath, arrowhead; matrix, arrow.
Mentions: Merino and Suffolk are two different sheep breeds. There is a dramatic difference in average wool fiber diameter between the two sheep breeds [20]. Consistent with the known wool quality difference between Merino and Suffolk, our wool fiber measurement showed that the superfine wool strain (SF) Merino had lower average wool fiber diameter than Suffolk sheep (SF sheep: 17.58 μm; Suffolk: 30.92 μm; P = 0.0009; Fig 1A). To obtain the clue for understanding the effects of FST gene on sheep wool quality traits, we performed the tissue expression analysis of FST gene in SF sheep and Suffolk sheep by real-time RT-PCR. The results showed that FST gene was ubiquitously expressed in the eleven tested adult tissues of SF sheep (Fig 1B), and comparatively highly expressed in the body side skin and ovary (Fig 1B). Skin expression comparison revealed that FST gene expression was 4.15-fold lower in SF sheep than in Suffolk sheep (P < 0.05; Fig 1C). Correlation analysis showed that skin FST gene expression was positively significantly correlated to the average wool fiber diameter (Pearson’s r = 0.987, P < 0.05), suggesting that FST gene expression variation may contribute to the variation of wool quality traits between SF sheep and Suffolk sheep. Immunohistochemical localization showed that FST was localized in inner root sheath (IRS) and matrix of hair follicle (HF) in both SF and Suffolk sheep (Fig 1D and 1E).

View Article: PubMed Central - PubMed

ABSTRACT

Follistatin (FST) is involved in hair follicle morphogenesis. However, its effects on hair traits are not clear. This study was designed to investigate the effects of FST gene single nucleotide polymorphisms (SNP) on wool quality traits in Chinese Merino sheep (Junken Type). We performed gene expression analysis, SNP detection, and association analysis of FST gene with sheep wool quality traits. The real-time RT-PCR analysis showed that FST gene was differentially expressed in adult skin between Chinese Merino sheep (Junken Type) and Suffolk sheep. Immunostaining showed that FST was localized in inner root sheath (IRS) and matrix of hair follicle (HF) in both SF and Suffolk sheep. Sequencing analysis identified a total of seven SNPs (termed SNPs 1&ndash;7) in the FST gene in Chinese Merino sheep (Junken Type). Association analysis showed that SNP2 (Chr 16. 25,633,662 G&gt;A) was significantly associated with average wool fiber diameter, wool fineness SD, and wool crimp (P &lt; 0.05). SNP4 (Chr 16. 25,633,569 C&gt;T) was significantly associated with wool fineness SD and CV of fiber diameter (P &lt; 0.05). Similarly, the haplotypes derived from these seven identified SNPs were also significantly associated with average wool fiber diameter, wool fineness SD, CV of fiber diameter, and wool crimp (P &lt; 0.05). Our results suggest that FST influences wool quality traits and its SNPs 2 and 4 might be useful markers for marker-assisted selection and sheep breeding.

No MeSH data available.