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Chemical constituents and anti-inflammatory activities of Maqian ( Zanthoxylum myriacanthum var. pubescens ) bark extracts

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ABSTRACT

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from the ethyl acetate extracts of Maqian bark by column chromatography and identified by chemical and spectral analyses. Rhoifoline B, zanthoxyline dimethoxy derivative, N-nortidine, nitidine, decarine are the major alkaloids. Both the petroleum ether and ethyl acetate extracts showed significant inhibition on NO production, which imply anti-inflammatory activity, in lipopolysaccharide-induced RAW 264.7 cells without cell toxicity. Decarine is the major anti-inflammatory constituent with NO IC50 values of 48.43 μM on RAW264.7 cells. The petroleum ether extract, the ethyl acetate extract and decarine showed anti-inflammatory activities through inhibiting TNF-α and IL-1β production in lipopolysaccharide-stimulated THP-1 cells without cell toxicity too. Decarine showed anti-inflammatory activity on human colon cells by reducing IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells. These results support the use of Maqian bark as a remedy for enteritis and colitis recorded by Dai medicine in China, and elucidate the major pharmacological compounds in Maqian bark.

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Effect of decarine on IL-6 and IL-8 production in IL-1β+TNF-α-induced Caco-2 cells.All values were means ± SD, n = 3. (*P < 0.05) *P < 0.05 indicated significant difference with cells treated by IL-1β and TNF-α. Dex: dexamethasone; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-alpha; IL-6: interleukin-6; IL-8: interleukin-8.
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f9: Effect of decarine on IL-6 and IL-8 production in IL-1β+TNF-α-induced Caco-2 cells.All values were means ± SD, n = 3. (*P < 0.05) *P < 0.05 indicated significant difference with cells treated by IL-1β and TNF-α. Dex: dexamethasone; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-alpha; IL-6: interleukin-6; IL-8: interleukin-8.

Mentions: Decarine showed significant inhibitions on IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells at the concentration of 20 μM (*P < 0.05, Fig. 9), which indicated that it has anti-inflammatory activity on human colon cells. Anti-inflammatory drug dexamethasone showed slight but not significant inhibitions on the IL-6 and IL-8 production at 5 μM, which meant large concentration of dexamethasone might be needed to decrease the IL-6 and IL-8 production.


Chemical constituents and anti-inflammatory activities of Maqian ( Zanthoxylum myriacanthum var. pubescens ) bark extracts
Effect of decarine on IL-6 and IL-8 production in IL-1β+TNF-α-induced Caco-2 cells.All values were means ± SD, n = 3. (*P < 0.05) *P < 0.05 indicated significant difference with cells treated by IL-1β and TNF-α. Dex: dexamethasone; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-alpha; IL-6: interleukin-6; IL-8: interleukin-8.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382921&req=5

f9: Effect of decarine on IL-6 and IL-8 production in IL-1β+TNF-α-induced Caco-2 cells.All values were means ± SD, n = 3. (*P < 0.05) *P < 0.05 indicated significant difference with cells treated by IL-1β and TNF-α. Dex: dexamethasone; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-alpha; IL-6: interleukin-6; IL-8: interleukin-8.
Mentions: Decarine showed significant inhibitions on IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells at the concentration of 20 μM (*P < 0.05, Fig. 9), which indicated that it has anti-inflammatory activity on human colon cells. Anti-inflammatory drug dexamethasone showed slight but not significant inhibitions on the IL-6 and IL-8 production at 5 μM, which meant large concentration of dexamethasone might be needed to decrease the IL-6 and IL-8 production.

View Article: PubMed Central - PubMed

ABSTRACT

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from the ethyl acetate extracts of Maqian bark by column chromatography and identified by chemical and spectral analyses. Rhoifoline B, zanthoxyline dimethoxy derivative, N-nortidine, nitidine, decarine are the major alkaloids. Both the petroleum ether and ethyl acetate extracts showed significant inhibition on NO production, which imply anti-inflammatory activity, in lipopolysaccharide-induced RAW 264.7 cells without cell toxicity. Decarine is the major anti-inflammatory constituent with NO IC50 values of 48.43&thinsp;&mu;M on RAW264.7 cells. The petroleum ether extract, the ethyl acetate extract and decarine showed anti-inflammatory activities through inhibiting TNF-&alpha; and IL-1&beta; production in lipopolysaccharide-stimulated THP-1 cells without cell toxicity too. Decarine showed anti-inflammatory activity on human colon cells by reducing IL-6 and IL-8 production in TNF-&alpha;+IL-1&beta;-induced Caco-2 cells. These results support the use of Maqian bark as a remedy for enteritis and colitis recorded by Dai medicine in China, and elucidate the major pharmacological compounds in Maqian bark.

No MeSH data available.


Related in: MedlinePlus