Limits...
Chemical constituents and anti-inflammatory activities of Maqian ( Zanthoxylum myriacanthum var. pubescens ) bark extracts

View Article: PubMed Central - PubMed

ABSTRACT

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from the ethyl acetate extracts of Maqian bark by column chromatography and identified by chemical and spectral analyses. Rhoifoline B, zanthoxyline dimethoxy derivative, N-nortidine, nitidine, decarine are the major alkaloids. Both the petroleum ether and ethyl acetate extracts showed significant inhibition on NO production, which imply anti-inflammatory activity, in lipopolysaccharide-induced RAW 264.7 cells without cell toxicity. Decarine is the major anti-inflammatory constituent with NO IC50 values of 48.43 μM on RAW264.7 cells. The petroleum ether extract, the ethyl acetate extract and decarine showed anti-inflammatory activities through inhibiting TNF-α and IL-1β production in lipopolysaccharide-stimulated THP-1 cells without cell toxicity too. Decarine showed anti-inflammatory activity on human colon cells by reducing IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells. These results support the use of Maqian bark as a remedy for enteritis and colitis recorded by Dai medicine in China, and elucidate the major pharmacological compounds in Maqian bark.

No MeSH data available.


Chemical structure of decarine (18)34.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5382921&req=5

f6: Chemical structure of decarine (18)34.

Mentions: According to the effect of the anti-inflammatory activity of extracts from Maqian bark, the inhibitory activity of the ethyl acetate extract was significantly higher than that of the other two Maqian bark extracts at all tested concentrations, indicating it had a better anti-inflammatory activity. Results showed that the petroleum ether extract also had a significant anti-inflammatory activity while the n-butanol extract had only very weak activity. Decarine (18) (Fig. 6) is the major anti-inflammatory active constituent in the ethyl acetate extract with IC50 values of 48.43 μM (14.67 μg/ml) (Table 3).


Chemical constituents and anti-inflammatory activities of Maqian ( Zanthoxylum myriacanthum var. pubescens ) bark extracts
Chemical structure of decarine (18)34.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382921&req=5

f6: Chemical structure of decarine (18)34.
Mentions: According to the effect of the anti-inflammatory activity of extracts from Maqian bark, the inhibitory activity of the ethyl acetate extract was significantly higher than that of the other two Maqian bark extracts at all tested concentrations, indicating it had a better anti-inflammatory activity. Results showed that the petroleum ether extract also had a significant anti-inflammatory activity while the n-butanol extract had only very weak activity. Decarine (18) (Fig. 6) is the major anti-inflammatory active constituent in the ethyl acetate extract with IC50 values of 48.43 μM (14.67 μg/ml) (Table 3).

View Article: PubMed Central - PubMed

ABSTRACT

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from the ethyl acetate extracts of Maqian bark by column chromatography and identified by chemical and spectral analyses. Rhoifoline B, zanthoxyline dimethoxy derivative, N-nortidine, nitidine, decarine are the major alkaloids. Both the petroleum ether and ethyl acetate extracts showed significant inhibition on NO production, which imply anti-inflammatory activity, in lipopolysaccharide-induced RAW 264.7 cells without cell toxicity. Decarine is the major anti-inflammatory constituent with NO IC50 values of 48.43 μM on RAW264.7 cells. The petroleum ether extract, the ethyl acetate extract and decarine showed anti-inflammatory activities through inhibiting TNF-α and IL-1β production in lipopolysaccharide-stimulated THP-1 cells without cell toxicity too. Decarine showed anti-inflammatory activity on human colon cells by reducing IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells. These results support the use of Maqian bark as a remedy for enteritis and colitis recorded by Dai medicine in China, and elucidate the major pharmacological compounds in Maqian bark.

No MeSH data available.