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Chemical constituents and anti-inflammatory activities of Maqian ( Zanthoxylum myriacanthum var. pubescens ) bark extracts

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ABSTRACT

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from the ethyl acetate extracts of Maqian bark by column chromatography and identified by chemical and spectral analyses. Rhoifoline B, zanthoxyline dimethoxy derivative, N-nortidine, nitidine, decarine are the major alkaloids. Both the petroleum ether and ethyl acetate extracts showed significant inhibition on NO production, which imply anti-inflammatory activity, in lipopolysaccharide-induced RAW 264.7 cells without cell toxicity. Decarine is the major anti-inflammatory constituent with NO IC50 values of 48.43 μM on RAW264.7 cells. The petroleum ether extract, the ethyl acetate extract and decarine showed anti-inflammatory activities through inhibiting TNF-α and IL-1β production in lipopolysaccharide-stimulated THP-1 cells without cell toxicity too. Decarine showed anti-inflammatory activity on human colon cells by reducing IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells. These results support the use of Maqian bark as a remedy for enteritis and colitis recorded by Dai medicine in China, and elucidate the major pharmacological compounds in Maqian bark.

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Effect of decarine on the cell viability of Caco-2 cells.All values were means ± SD, n = 3. Dex: dexamethasone; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-alpha.
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f4: Effect of decarine on the cell viability of Caco-2 cells.All values were means ± SD, n = 3. Dex: dexamethasone; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-alpha.

Mentions: Decarine did not reduce viability of Caco-2 cells up to the concentration of 40 μM (Fig. 4). The results indicated that decarine isolated from the ethyl acetate extract of Zanthoxylum myriacanthum var. pubescens did not exhibit any cytotoxic effect at the employed concentrations of 10. 20 and 40 μM.


Chemical constituents and anti-inflammatory activities of Maqian ( Zanthoxylum myriacanthum var. pubescens ) bark extracts
Effect of decarine on the cell viability of Caco-2 cells.All values were means ± SD, n = 3. Dex: dexamethasone; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-alpha.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5382921&req=5

f4: Effect of decarine on the cell viability of Caco-2 cells.All values were means ± SD, n = 3. Dex: dexamethasone; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-alpha.
Mentions: Decarine did not reduce viability of Caco-2 cells up to the concentration of 40 μM (Fig. 4). The results indicated that decarine isolated from the ethyl acetate extract of Zanthoxylum myriacanthum var. pubescens did not exhibit any cytotoxic effect at the employed concentrations of 10. 20 and 40 μM.

View Article: PubMed Central - PubMed

ABSTRACT

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from the ethyl acetate extracts of Maqian bark by column chromatography and identified by chemical and spectral analyses. Rhoifoline B, zanthoxyline dimethoxy derivative, N-nortidine, nitidine, decarine are the major alkaloids. Both the petroleum ether and ethyl acetate extracts showed significant inhibition on NO production, which imply anti-inflammatory activity, in lipopolysaccharide-induced RAW 264.7 cells without cell toxicity. Decarine is the major anti-inflammatory constituent with NO IC50 values of 48.43 μM on RAW264.7 cells. The petroleum ether extract, the ethyl acetate extract and decarine showed anti-inflammatory activities through inhibiting TNF-α and IL-1β production in lipopolysaccharide-stimulated THP-1 cells without cell toxicity too. Decarine showed anti-inflammatory activity on human colon cells by reducing IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells. These results support the use of Maqian bark as a remedy for enteritis and colitis recorded by Dai medicine in China, and elucidate the major pharmacological compounds in Maqian bark.

No MeSH data available.


Related in: MedlinePlus