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Chemical constituents and anti-inflammatory activities of Maqian ( Zanthoxylum myriacanthum var. pubescens ) bark extracts

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ABSTRACT

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from the ethyl acetate extracts of Maqian bark by column chromatography and identified by chemical and spectral analyses. Rhoifoline B, zanthoxyline dimethoxy derivative, N-nortidine, nitidine, decarine are the major alkaloids. Both the petroleum ether and ethyl acetate extracts showed significant inhibition on NO production, which imply anti-inflammatory activity, in lipopolysaccharide-induced RAW 264.7 cells without cell toxicity. Decarine is the major anti-inflammatory constituent with NO IC50 values of 48.43 μM on RAW264.7 cells. The petroleum ether extract, the ethyl acetate extract and decarine showed anti-inflammatory activities through inhibiting TNF-α and IL-1β production in lipopolysaccharide-stimulated THP-1 cells without cell toxicity too. Decarine showed anti-inflammatory activity on human colon cells by reducing IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells. These results support the use of Maqian bark as a remedy for enteritis and colitis recorded by Dai medicine in China, and elucidate the major pharmacological compounds in Maqian bark.

No MeSH data available.


Effect of the extracts of Zanthoxylum myriacanthum var. pubescens on the cell viability of RAW 264.7 macrophages.All values were means ± SD, n = 3. Dex: dexamethasone.
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f2: Effect of the extracts of Zanthoxylum myriacanthum var. pubescens on the cell viability of RAW 264.7 macrophages.All values were means ± SD, n = 3. Dex: dexamethasone.

Mentions: The effect of the four kinds of Maqian bark extracts on cell viability was determined by the MTS assay. All extracts showed no effects on the cell viability of murine RAW 264.7 macrophages and human THP-1 cells at 10 μg/ml, 20 μg/ml, 40 μg/ml, 80 μg/ml (Figs 2 and 3). The ethyl acetate extract reduced cell viability slightly at 80 μg/ml. However, the viabilities of cells treated with 80 μg/ml ethyl acetate extract were not significantly different from those of the untreated control cells, as tested by Dunnett’s multiple comparison tests, implying that the anti-inflammatory activities of the extracts up to 80 μg/ml were not implicated with cell toxicity. Therefore, we inferred that concentrations up to 80 μg/ml of the extracts could be safe for the development of Maqian bark as a drug.


Chemical constituents and anti-inflammatory activities of Maqian ( Zanthoxylum myriacanthum var. pubescens ) bark extracts
Effect of the extracts of Zanthoxylum myriacanthum var. pubescens on the cell viability of RAW 264.7 macrophages.All values were means ± SD, n = 3. Dex: dexamethasone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382921&req=5

f2: Effect of the extracts of Zanthoxylum myriacanthum var. pubescens on the cell viability of RAW 264.7 macrophages.All values were means ± SD, n = 3. Dex: dexamethasone.
Mentions: The effect of the four kinds of Maqian bark extracts on cell viability was determined by the MTS assay. All extracts showed no effects on the cell viability of murine RAW 264.7 macrophages and human THP-1 cells at 10 μg/ml, 20 μg/ml, 40 μg/ml, 80 μg/ml (Figs 2 and 3). The ethyl acetate extract reduced cell viability slightly at 80 μg/ml. However, the viabilities of cells treated with 80 μg/ml ethyl acetate extract were not significantly different from those of the untreated control cells, as tested by Dunnett’s multiple comparison tests, implying that the anti-inflammatory activities of the extracts up to 80 μg/ml were not implicated with cell toxicity. Therefore, we inferred that concentrations up to 80 μg/ml of the extracts could be safe for the development of Maqian bark as a drug.

View Article: PubMed Central - PubMed

ABSTRACT

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from the ethyl acetate extracts of Maqian bark by column chromatography and identified by chemical and spectral analyses. Rhoifoline B, zanthoxyline dimethoxy derivative, N-nortidine, nitidine, decarine are the major alkaloids. Both the petroleum ether and ethyl acetate extracts showed significant inhibition on NO production, which imply anti-inflammatory activity, in lipopolysaccharide-induced RAW 264.7 cells without cell toxicity. Decarine is the major anti-inflammatory constituent with NO IC50 values of 48.43 μM on RAW264.7 cells. The petroleum ether extract, the ethyl acetate extract and decarine showed anti-inflammatory activities through inhibiting TNF-α and IL-1β production in lipopolysaccharide-stimulated THP-1 cells without cell toxicity too. Decarine showed anti-inflammatory activity on human colon cells by reducing IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells. These results support the use of Maqian bark as a remedy for enteritis and colitis recorded by Dai medicine in China, and elucidate the major pharmacological compounds in Maqian bark.

No MeSH data available.