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Chemical constituents and anti-inflammatory activities of Maqian ( Zanthoxylum myriacanthum var. pubescens ) bark extracts

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ABSTRACT

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from the ethyl acetate extracts of Maqian bark by column chromatography and identified by chemical and spectral analyses. Rhoifoline B, zanthoxyline dimethoxy derivative, N-nortidine, nitidine, decarine are the major alkaloids. Both the petroleum ether and ethyl acetate extracts showed significant inhibition on NO production, which imply anti-inflammatory activity, in lipopolysaccharide-induced RAW 264.7 cells without cell toxicity. Decarine is the major anti-inflammatory constituent with NO IC50 values of 48.43 μM on RAW264.7 cells. The petroleum ether extract, the ethyl acetate extract and decarine showed anti-inflammatory activities through inhibiting TNF-α and IL-1β production in lipopolysaccharide-stimulated THP-1 cells without cell toxicity too. Decarine showed anti-inflammatory activity on human colon cells by reducing IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells. These results support the use of Maqian bark as a remedy for enteritis and colitis recorded by Dai medicine in China, and elucidate the major pharmacological compounds in Maqian bark.

No MeSH data available.


The total ion chromatogram (TIC) of GC-MS of petroleum ether extract from Zanthoxylum myriacanthum var.pubescens bark.
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f1: The total ion chromatogram (TIC) of GC-MS of petroleum ether extract from Zanthoxylum myriacanthum var.pubescens bark.

Mentions: Forty four compounds (Table 1, Fig. 1) were identified in the petroleum ether extract, representing 75.542% of the petroleum ether extract of Maqian bark. Among them, 3(2H)-benzofuranone (26.173%), asarinin (6.484%), (dimethoxymethyl)-3-methoxy-benzene (5.443%), hexadecanoic acid (4.378%), 9,12-octadecadienoic acid (4.119%), nerolidol (2.451%), 9-octadecenoic acid (2.138%), (−)-caryophyllene oxide (2.023%), 2-tridecanone (1.349%), bornyl acetate (1.250%), (+)-spathulenol (1.246%), hexadecanoic acid, methyl ester (1.133%), were the major compounds, which accounted for 58.187% of the total compounds in the extract.


Chemical constituents and anti-inflammatory activities of Maqian ( Zanthoxylum myriacanthum var. pubescens ) bark extracts
The total ion chromatogram (TIC) of GC-MS of petroleum ether extract from Zanthoxylum myriacanthum var.pubescens bark.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382921&req=5

f1: The total ion chromatogram (TIC) of GC-MS of petroleum ether extract from Zanthoxylum myriacanthum var.pubescens bark.
Mentions: Forty four compounds (Table 1, Fig. 1) were identified in the petroleum ether extract, representing 75.542% of the petroleum ether extract of Maqian bark. Among them, 3(2H)-benzofuranone (26.173%), asarinin (6.484%), (dimethoxymethyl)-3-methoxy-benzene (5.443%), hexadecanoic acid (4.378%), 9,12-octadecadienoic acid (4.119%), nerolidol (2.451%), 9-octadecenoic acid (2.138%), (−)-caryophyllene oxide (2.023%), 2-tridecanone (1.349%), bornyl acetate (1.250%), (+)-spathulenol (1.246%), hexadecanoic acid, methyl ester (1.133%), were the major compounds, which accounted for 58.187% of the total compounds in the extract.

View Article: PubMed Central - PubMed

ABSTRACT

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from the ethyl acetate extracts of Maqian bark by column chromatography and identified by chemical and spectral analyses. Rhoifoline B, zanthoxyline dimethoxy derivative, N-nortidine, nitidine, decarine are the major alkaloids. Both the petroleum ether and ethyl acetate extracts showed significant inhibition on NO production, which imply anti-inflammatory activity, in lipopolysaccharide-induced RAW 264.7 cells without cell toxicity. Decarine is the major anti-inflammatory constituent with NO IC50 values of 48.43 μM on RAW264.7 cells. The petroleum ether extract, the ethyl acetate extract and decarine showed anti-inflammatory activities through inhibiting TNF-α and IL-1β production in lipopolysaccharide-stimulated THP-1 cells without cell toxicity too. Decarine showed anti-inflammatory activity on human colon cells by reducing IL-6 and IL-8 production in TNF-α+IL-1β-induced Caco-2 cells. These results support the use of Maqian bark as a remedy for enteritis and colitis recorded by Dai medicine in China, and elucidate the major pharmacological compounds in Maqian bark.

No MeSH data available.