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The effect of hydrolysates of proteins from rice milk on the physiological response of enterocytes and on the adhesion of bacteria from healthy and allergic people – an in vitro study

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ABSTRACT

Designing an optimal diet requires knowledge of the biological activity of food products, particularly in relation to people with food allergies. The hypothesis, which constitutes the basis of this thesis, states that the peptides and glycopeptides released from proteins by enzymatic hydrolysis are able to change the quantity and quality of the human gastrointestinal ecosystem. Such substrates may interfere with adhesion to the intestinal epithelium microbiota and alter enterocytic metabolic activity. The aim of this study was to determine the effect of protein hydrolysates from rice milk substitute on gut epithelial cells and the intestinal microbiota of healthy people and ones suffering from an allergy to milk. The following experimental work applied systems that reflect the conditions occurring in the gastrointestinal tract.

No MeSH data available.


Related in: MedlinePlus

Impact of hydrolysates of proteins from rice milk substitute – RH on the adhesion of mixed bacterial suspension consisting of Enterobacteriaceae, Lactobacillus/Enterococcus to the surface of Caco-2/HT29MTX cells. A) Bacteria obtained from healthy people; B) Bacteria obtained from allergic people
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f0006: Impact of hydrolysates of proteins from rice milk substitute – RH on the adhesion of mixed bacterial suspension consisting of Enterobacteriaceae, Lactobacillus/Enterococcus to the surface of Caco-2/HT29MTX cells. A) Bacteria obtained from healthy people; B) Bacteria obtained from allergic people

Mentions: Analysed hydrolysates had no impact on the total number of adhering bacteria (TBN) in mixed suspension obtained from healthy people (Fig. 6A). Whereas they stimulated the total number of adhering bacteria in mixed suspension obtained from allergic people in the Caco-2/HT-29-MTX co-culture (Fig. 6B). Regardless of the total number of bacteria adhering to small intestinal cells, analysed hydrolysates had no impact on the profile (proportions) of adhering bacteria regardless of their origin, leaving lactobacilli/enterococci more abundantly immobilised in comparison to enterobacteria (Fig. 6A, B).


The effect of hydrolysates of proteins from rice milk on the physiological response of enterocytes and on the adhesion of bacteria from healthy and allergic people – an in vitro study
Impact of hydrolysates of proteins from rice milk substitute – RH on the adhesion of mixed bacterial suspension consisting of Enterobacteriaceae, Lactobacillus/Enterococcus to the surface of Caco-2/HT29MTX cells. A) Bacteria obtained from healthy people; B) Bacteria obtained from allergic people
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382882&req=5

f0006: Impact of hydrolysates of proteins from rice milk substitute – RH on the adhesion of mixed bacterial suspension consisting of Enterobacteriaceae, Lactobacillus/Enterococcus to the surface of Caco-2/HT29MTX cells. A) Bacteria obtained from healthy people; B) Bacteria obtained from allergic people
Mentions: Analysed hydrolysates had no impact on the total number of adhering bacteria (TBN) in mixed suspension obtained from healthy people (Fig. 6A). Whereas they stimulated the total number of adhering bacteria in mixed suspension obtained from allergic people in the Caco-2/HT-29-MTX co-culture (Fig. 6B). Regardless of the total number of bacteria adhering to small intestinal cells, analysed hydrolysates had no impact on the profile (proportions) of adhering bacteria regardless of their origin, leaving lactobacilli/enterococci more abundantly immobilised in comparison to enterobacteria (Fig. 6A, B).

View Article: PubMed Central - PubMed

ABSTRACT

Designing an optimal diet requires knowledge of the biological activity of food products, particularly in relation to people with food allergies. The hypothesis, which constitutes the basis of this thesis, states that the peptides and glycopeptides released from proteins by enzymatic hydrolysis are able to change the quantity and quality of the human gastrointestinal ecosystem. Such substrates may interfere with adhesion to the intestinal epithelium microbiota and alter enterocytic metabolic activity. The aim of this study was to determine the effect of protein hydrolysates from rice milk substitute on gut epithelial cells and the intestinal microbiota of healthy people and ones suffering from an allergy to milk. The following experimental work applied systems that reflect the conditions occurring in the gastrointestinal tract.

No MeSH data available.


Related in: MedlinePlus