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The effect of hydrolysates of proteins from rice milk on the physiological response of enterocytes and on the adhesion of bacteria from healthy and allergic people – an in vitro study

View Article: PubMed Central - PubMed

ABSTRACT

Designing an optimal diet requires knowledge of the biological activity of food products, particularly in relation to people with food allergies. The hypothesis, which constitutes the basis of this thesis, states that the peptides and glycopeptides released from proteins by enzymatic hydrolysis are able to change the quantity and quality of the human gastrointestinal ecosystem. Such substrates may interfere with adhesion to the intestinal epithelium microbiota and alter enterocytic metabolic activity. The aim of this study was to determine the effect of protein hydrolysates from rice milk substitute on gut epithelial cells and the intestinal microbiota of healthy people and ones suffering from an allergy to milk. The following experimental work applied systems that reflect the conditions occurring in the gastrointestinal tract.

No MeSH data available.


Small intestinal barrier models used in the study
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f0001: Small intestinal barrier models used in the study

Mentions: The small intestinal barrier model used in this study consisted of Caco-2 cells, representing enterocytes, HT-29-MTX cells, representing Goblet cells and intestinal bacteria, obtained from healthy and allergic people, which were adhered to the epithelial surface (Fig. 1). Such models were used to analyse the physiological response of epithelial cells (metabolic and proliferative activity, IL-8 secretion, transepithelial resistance, and gene expression) and the adhesive potential of bacteria to RH hydrolysates.


The effect of hydrolysates of proteins from rice milk on the physiological response of enterocytes and on the adhesion of bacteria from healthy and allergic people – an in vitro study
Small intestinal barrier models used in the study
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382882&req=5

f0001: Small intestinal barrier models used in the study
Mentions: The small intestinal barrier model used in this study consisted of Caco-2 cells, representing enterocytes, HT-29-MTX cells, representing Goblet cells and intestinal bacteria, obtained from healthy and allergic people, which were adhered to the epithelial surface (Fig. 1). Such models were used to analyse the physiological response of epithelial cells (metabolic and proliferative activity, IL-8 secretion, transepithelial resistance, and gene expression) and the adhesive potential of bacteria to RH hydrolysates.

View Article: PubMed Central - PubMed

ABSTRACT

Designing an optimal diet requires knowledge of the biological activity of food products, particularly in relation to people with food allergies. The hypothesis, which constitutes the basis of this thesis, states that the peptides and glycopeptides released from proteins by enzymatic hydrolysis are able to change the quantity and quality of the human gastrointestinal ecosystem. Such substrates may interfere with adhesion to the intestinal epithelium microbiota and alter enterocytic metabolic activity. The aim of this study was to determine the effect of protein hydrolysates from rice milk substitute on gut epithelial cells and the intestinal microbiota of healthy people and ones suffering from an allergy to milk. The following experimental work applied systems that reflect the conditions occurring in the gastrointestinal tract.

No MeSH data available.