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Ellagic acid alleviates adjuvant induced arthritis by modulation of pro- and anti-inflammatory cytokines

View Article: PubMed Central - PubMed

ABSTRACT

Rheumatoid arthritis (RA) is a chronic inflammatory disease of unknown aetiology, but it is now clear that pro-inflammatory cytokines play a central role in its pathogenesis. Ellagic acid (EA) has a variety of biological activities including anti-oxidant, anti-inflammatory, and anti-cancer properties. The aim of the present study was to evaluate the potential effect of ellagic acid on the prevention and/or treatment of adjuvant induced arthritis (AIA) model in mice. Ellagic acid treatment was started one week before AIA induction and continued for three weeks after induction of AIA. Ellagic acid treatment significantly (p < 0.01) inhibited foot paw oedematous swelling and attenuated AIA-associated pathology. Ellagic acid significantly (p < 0.01) reduced serum levels of pro-inflammatory cytokines: interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and interleukin 17 (IL-17). However, serum levels of IL-10 and interferon γ (IFN-γ) significantly increased (p < 0.01 and p < 0.05, respectively), while serum level of transforming growth factor β (TGF-β) did not significantly alter with EA treatment. In conclusion, these results suggest that EA attenuated AIA-associated pathology in the mouse model by downregulation of pro-inflammatory cytokines and upregulation of anti-inflammatory cytokines.

No MeSH data available.


Related in: MedlinePlus

Histopathological evaluation of ellagic acid treatment on adjuvant induced arthritis in mice. Representative results showing the histopathological picture of hind paws and digits of normal control (NC), normal treated (NT), arthritic control (AC) and arthritic treated (AT) mice in H&E (100×) stained sections. The normal histological picture of hind paws joints was presents in both NC and NT mice. AC mice showed synovial hyperplasia, inflammatory cells infiltration, pannus formation, and cartilage destruction; whereas AT mice revealed less severity of arthritis pathology
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f0001: Histopathological evaluation of ellagic acid treatment on adjuvant induced arthritis in mice. Representative results showing the histopathological picture of hind paws and digits of normal control (NC), normal treated (NT), arthritic control (AC) and arthritic treated (AT) mice in H&E (100×) stained sections. The normal histological picture of hind paws joints was presents in both NC and NT mice. AC mice showed synovial hyperplasia, inflammatory cells infiltration, pannus formation, and cartilage destruction; whereas AT mice revealed less severity of arthritis pathology

Mentions: In order to investigate the effect of EA treatment on arthritic histopathology, the right ankles and hind paws were removed after sacrifice on the 21st day post arthritis induction and processed for histopathological examinations. As shown in Fig. 1, H&E stained sections of paws and digits tissues from both normal control (NC) and normal treated (NT) mice revealed no inflammation. However, stained sections of arthritic control (AC) mice revealed marked histopathological changes in the form of synovial hyperplasia, with a large number of inflammatory cells (lymphocytes, macrophages, and sometimes plasma cells), extensive pannus formation, and severe cartilage destruction. On the other hand, arthritic-treated (AT) mice showed less severe arthritis pathology with moderate inflammation (Fig. 1).


Ellagic acid alleviates adjuvant induced arthritis by modulation of pro- and anti-inflammatory cytokines
Histopathological evaluation of ellagic acid treatment on adjuvant induced arthritis in mice. Representative results showing the histopathological picture of hind paws and digits of normal control (NC), normal treated (NT), arthritic control (AC) and arthritic treated (AT) mice in H&E (100×) stained sections. The normal histological picture of hind paws joints was presents in both NC and NT mice. AC mice showed synovial hyperplasia, inflammatory cells infiltration, pannus formation, and cartilage destruction; whereas AT mice revealed less severity of arthritis pathology
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382880&req=5

f0001: Histopathological evaluation of ellagic acid treatment on adjuvant induced arthritis in mice. Representative results showing the histopathological picture of hind paws and digits of normal control (NC), normal treated (NT), arthritic control (AC) and arthritic treated (AT) mice in H&E (100×) stained sections. The normal histological picture of hind paws joints was presents in both NC and NT mice. AC mice showed synovial hyperplasia, inflammatory cells infiltration, pannus formation, and cartilage destruction; whereas AT mice revealed less severity of arthritis pathology
Mentions: In order to investigate the effect of EA treatment on arthritic histopathology, the right ankles and hind paws were removed after sacrifice on the 21st day post arthritis induction and processed for histopathological examinations. As shown in Fig. 1, H&E stained sections of paws and digits tissues from both normal control (NC) and normal treated (NT) mice revealed no inflammation. However, stained sections of arthritic control (AC) mice revealed marked histopathological changes in the form of synovial hyperplasia, with a large number of inflammatory cells (lymphocytes, macrophages, and sometimes plasma cells), extensive pannus formation, and severe cartilage destruction. On the other hand, arthritic-treated (AT) mice showed less severe arthritis pathology with moderate inflammation (Fig. 1).

View Article: PubMed Central - PubMed

ABSTRACT

Rheumatoid arthritis (RA) is a chronic inflammatory disease of unknown aetiology, but it is now clear that pro-inflammatory cytokines play a central role in its pathogenesis. Ellagic acid (EA) has a variety of biological activities including anti-oxidant, anti-inflammatory, and anti-cancer properties. The aim of the present study was to evaluate the potential effect of ellagic acid on the prevention and/or treatment of adjuvant induced arthritis (AIA) model in mice. Ellagic acid treatment was started one week before AIA induction and continued for three weeks after induction of AIA. Ellagic acid treatment significantly (p < 0.01) inhibited foot paw oedematous swelling and attenuated AIA-associated pathology. Ellagic acid significantly (p < 0.01) reduced serum levels of pro-inflammatory cytokines: interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and interleukin 17 (IL-17). However, serum levels of IL-10 and interferon γ (IFN-γ) significantly increased (p < 0.01 and p < 0.05, respectively), while serum level of transforming growth factor β (TGF-β) did not significantly alter with EA treatment. In conclusion, these results suggest that EA attenuated AIA-associated pathology in the mouse model by downregulation of pro-inflammatory cytokines and upregulation of anti-inflammatory cytokines.

No MeSH data available.


Related in: MedlinePlus