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Assessment of interleukin-17A, C5a and RANTES for early diagnosis of neonatal sepsis – a preliminary study

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ABSTRACT

The aim of the present study was to investigate serum levels of novel markers: interleukin 17A (IL-17A), anaphylatoxin C5a and chemokine regulated upon activation normal T-cell expressed and secreted (RANTES) in neonates with clinically suspected early-onset neonatal sepsis (EONS), and to compare their values with those of non-infected neonates. Eighteen neonates with clinical signs and symptoms of EONS were enrolled in this study. Fifty healthy, non-infected neonates served as the control group. In all neonates serum levels of IL-17A, C5a and RANTES were measured by solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). At the time of investigation serum levels of anaphylatoxin C5a were significantly higher in neonates with clinical symptoms of EONS than in non-infected neonates (median 65.35 vs. 50.4 ng/ml, p = 0.034), whereas levels of RANTES were similar and levels of IL-17A were under detection limit of the method. Based on these preliminary results, serum levels of C5a may be a useful marker of inflammation in early onset neonatal sepsis. Because traditional methods of microbiological diagnostics in EONS are frequently unsuccessful, the search for an alternative laboratory biomarkers is of great clinical importance. Thus, there is a strong need for further studies evaluating usefulness of this anaphylatoxin in EONS diagnosis on a larger group of patients.

No MeSH data available.


Scatter plots representing C5a (A) and RANTES (B) levels in serum of neonates. The C5a levels measured in septic neonates (EONS) vs. non-infected neonates (healthy controls) showed a significant difference (p = 0.034; by Mann-Whitney U test)
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f0001: Scatter plots representing C5a (A) and RANTES (B) levels in serum of neonates. The C5a levels measured in septic neonates (EONS) vs. non-infected neonates (healthy controls) showed a significant difference (p = 0.034; by Mann-Whitney U test)

Mentions: At the time of sepsis investigation serum levels of C5a in the EONS group were significantly higher compared to those in the non-infected group (median 65.35 vs. 50.4 ng/ml, p = 0.034; Fig. 1A), whereas no significant difference in RANTES levels between the two groups were found (median 188.95 vs. 195.2 ng/ml; p = 0.444; Fig. 1B).


Assessment of interleukin-17A, C5a and RANTES for early diagnosis of neonatal sepsis – a preliminary study
Scatter plots representing C5a (A) and RANTES (B) levels in serum of neonates. The C5a levels measured in septic neonates (EONS) vs. non-infected neonates (healthy controls) showed a significant difference (p = 0.034; by Mann-Whitney U test)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382877&req=5

f0001: Scatter plots representing C5a (A) and RANTES (B) levels in serum of neonates. The C5a levels measured in septic neonates (EONS) vs. non-infected neonates (healthy controls) showed a significant difference (p = 0.034; by Mann-Whitney U test)
Mentions: At the time of sepsis investigation serum levels of C5a in the EONS group were significantly higher compared to those in the non-infected group (median 65.35 vs. 50.4 ng/ml, p = 0.034; Fig. 1A), whereas no significant difference in RANTES levels between the two groups were found (median 188.95 vs. 195.2 ng/ml; p = 0.444; Fig. 1B).

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the present study was to investigate serum levels of novel markers: interleukin 17A (IL-17A), anaphylatoxin C5a and chemokine regulated upon activation normal T-cell expressed and secreted (RANTES) in neonates with clinically suspected early-onset neonatal sepsis (EONS), and to compare their values with those of non-infected neonates. Eighteen neonates with clinical signs and symptoms of EONS were enrolled in this study. Fifty healthy, non-infected neonates served as the control group. In all neonates serum levels of IL-17A, C5a and RANTES were measured by solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). At the time of investigation serum levels of anaphylatoxin C5a were significantly higher in neonates with clinical symptoms of EONS than in non-infected neonates (median 65.35 vs. 50.4 ng/ml, p = 0.034), whereas levels of RANTES were similar and levels of IL-17A were under detection limit of the method. Based on these preliminary results, serum levels of C5a may be a useful marker of inflammation in early onset neonatal sepsis. Because traditional methods of microbiological diagnostics in EONS are frequently unsuccessful, the search for an alternative laboratory biomarkers is of great clinical importance. Thus, there is a strong need for further studies evaluating usefulness of this anaphylatoxin in EONS diagnosis on a larger group of patients.

No MeSH data available.