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Preliminary Pharmacognostic and Phytochemical Investigation of Blepharis sindica -T. Anders Seeds

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ABSTRACT

Background:: Blepharis sindica – T. Anders of family Acanthaceae is an important medicinal plant which is mainly used as an invigorating tonic, given to cattle to increase milk production and its roots are used for urinary discharge and dysmenorrhea. It is commonly known as “Bhaṅgārī”. It is extensively used traditionally as Vājīkāraka and Vṛṣya (Aphrodisiac) by vaidyas of Rajasthan and Gujarat. The seeds of Blepharis sindica T. Anders are the parts of the plant valued for their medicinal value.

Aims:: The present investigation deals with the pharmacognostic studies of the root, stem, seed and seed powder.

Materials and methods:: The study includes macroscopy, microscopy, preliminary phytochemical analysis and physicochemical evaluation using methods given in Indian Ayurvedic Pharmacopoeia.

Results:: Physicochemical parameters such as total ash value, acid insoluble ash value and water soluble ash value were determined to be 21.449 ± 2.629%w/w, 0.986%w/w, 18.746%w/w respectively. Preliminary phytochemical analysis of extracts were carried out. Different extracts contain carbohydrate, protein, alkaloids, phenols, saponins and steroids prominently. Calcium, zinc, potassium and iron were also present. These secondary metabolites are the active constituents and may be responsible for B. sindica's pharmacological activities. Chief characters of transverse section of stem include ringed vascular bundles, radial striped pith rays and parenchymal pith. Seed shows presence of scalariform tracheids and lignified walls.

Conclusions:: The result of the present study can be useful for the identification and preparation of a monograph of the plant.

No MeSH data available.


Dried plant of Blepharis sindica T. Anders showing dried fruits and stem
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Figure 1: Dried plant of Blepharis sindica T. Anders showing dried fruits and stem

Mentions: The plant specimen was collected from natural habitat of Gudhamalani, Barmer (Rajasthan) [Figure 1]. The taxonomic identification of the plant was confirmed by Dr. J. P. Parmar, Scientist ā€œEā€ and Head of office, Botanical Survey of India, Jodhpur Rajasthan vide Letter No. BSI/AZRC19014/SE-1/Estt./549, dated 27.01.2010 and the voucher specimen of the plant were deposited in the crude drug museum of the Department of Dravyaguna Vigyan, National Institute of Ayurveda, Jaipur. Prior to all analysis, all the raw materials were cleaned to remove any foreign materials and dust. The samples were subjected to organoleptic, microscopic and phytochemical study so as to generate inputs that can be considered for laying down standards in respect of this plant. The seeds were dried in shade, powdered in an electric grinder, passed through 120 mesh sieves and stored in an airtight container for organoleptic, physicochemical and phytochemical studies. Physicochemical and preliminary phytochemical screening of the seeds was carried out on the coarse powder according to the standard method. The microscopy of root, stem, seed and seed powder was done. The various parameters analyzed are as under.


Preliminary Pharmacognostic and Phytochemical Investigation of Blepharis sindica -T. Anders Seeds
Dried plant of Blepharis sindica T. Anders showing dried fruits and stem
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382822&req=5

Figure 1: Dried plant of Blepharis sindica T. Anders showing dried fruits and stem
Mentions: The plant specimen was collected from natural habitat of Gudhamalani, Barmer (Rajasthan) [Figure 1]. The taxonomic identification of the plant was confirmed by Dr. J. P. Parmar, Scientist ā€œEā€ and Head of office, Botanical Survey of India, Jodhpur Rajasthan vide Letter No. BSI/AZRC19014/SE-1/Estt./549, dated 27.01.2010 and the voucher specimen of the plant were deposited in the crude drug museum of the Department of Dravyaguna Vigyan, National Institute of Ayurveda, Jaipur. Prior to all analysis, all the raw materials were cleaned to remove any foreign materials and dust. The samples were subjected to organoleptic, microscopic and phytochemical study so as to generate inputs that can be considered for laying down standards in respect of this plant. The seeds were dried in shade, powdered in an electric grinder, passed through 120 mesh sieves and stored in an airtight container for organoleptic, physicochemical and phytochemical studies. Physicochemical and preliminary phytochemical screening of the seeds was carried out on the coarse powder according to the standard method. The microscopy of root, stem, seed and seed powder was done. The various parameters analyzed are as under.

View Article: PubMed Central - PubMed

ABSTRACT

Background:: Blepharis sindica – T. Anders of family Acanthaceae is an important medicinal plant which is mainly used as an invigorating tonic, given to cattle to increase milk production and its roots are used for urinary discharge and dysmenorrhea. It is commonly known as “Bhaṅgārī”. It is extensively used traditionally as Vājīkāraka and Vṛṣya (Aphrodisiac) by vaidyas of Rajasthan and Gujarat. The seeds of Blepharis sindica T. Anders are the parts of the plant valued for their medicinal value.

Aims:: The present investigation deals with the pharmacognostic studies of the root, stem, seed and seed powder.

Materials and methods:: The study includes macroscopy, microscopy, preliminary phytochemical analysis and physicochemical evaluation using methods given in Indian Ayurvedic Pharmacopoeia.

Results:: Physicochemical parameters such as total ash value, acid insoluble ash value and water soluble ash value were determined to be 21.449 ± 2.629%w/w, 0.986%w/w, 18.746%w/w respectively. Preliminary phytochemical analysis of extracts were carried out. Different extracts contain carbohydrate, protein, alkaloids, phenols, saponins and steroids prominently. Calcium, zinc, potassium and iron were also present. These secondary metabolites are the active constituents and may be responsible for B. sindica's pharmacological activities. Chief characters of transverse section of stem include ringed vascular bundles, radial striped pith rays and parenchymal pith. Seed shows presence of scalariform tracheids and lignified walls.

Conclusions:: The result of the present study can be useful for the identification and preparation of a monograph of the plant.

No MeSH data available.