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Molecular Verification of New World Mansonella perstans Parasitemias

View Article: PubMed Central - PubMed

ABSTRACT

We obtained ribosomal and mitochondrial DNA sequences from residents of Amazonas state, Brazil, with Mansonella parasitemias. Phylogenetic analysis of these sequences confirm that M. ozzardi and M. perstans parasites occur in sympatry and reveal the close relationship between M. perstans in Africa and Brazil, providing insights into the parasite’s New World origins.

No MeSH data available.


Maximum-likelihood phylogenetic trees showing the relationship between Mansonella parasites from Amazon region of Brazil (Amazonas state) and some of their closest relatives. A) Ribosomal internal transcribed spacer 1–based phylogeny. B) Mitochondrial cytochrome c oxidase subunit 1–based phylogeny. C) Mitochondrial 12S-based phylogeny. All 3 trees were prepared by using DNA sequence alignments and PHYLIP version 3.67 (http://evolution.genetics.washington.edu/phylip.html). Black circles indicate significant bootstrap-supported nodes as a percentage of 1,000 pseudoreplicates. Solid boxes indicate M. perstans and dashed boxes M. ozzardi sequences generated for this study and used in the construction of the displayed trees. Scale bars indicate nucleotide substitutions per site. These sequence have been submitted to GenBank and EMBL (accession nos.: M. perstans cytochrome c oxidase subunit 1, LT623909; M. ozzardi cytochrome c oxidase subunit 1, LT623910; M. perstans 12S, LT623913; M. ozzardi 12S, LT623914; M. perstans internal transcribed spacer 1, LT623911; and M. ozzardi internal transcribed spacer 1, LT623912).
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Figure 1: Maximum-likelihood phylogenetic trees showing the relationship between Mansonella parasites from Amazon region of Brazil (Amazonas state) and some of their closest relatives. A) Ribosomal internal transcribed spacer 1–based phylogeny. B) Mitochondrial cytochrome c oxidase subunit 1–based phylogeny. C) Mitochondrial 12S-based phylogeny. All 3 trees were prepared by using DNA sequence alignments and PHYLIP version 3.67 (http://evolution.genetics.washington.edu/phylip.html). Black circles indicate significant bootstrap-supported nodes as a percentage of 1,000 pseudoreplicates. Solid boxes indicate M. perstans and dashed boxes M. ozzardi sequences generated for this study and used in the construction of the displayed trees. Scale bars indicate nucleotide substitutions per site. These sequence have been submitted to GenBank and EMBL (accession nos.: M. perstans cytochrome c oxidase subunit 1, LT623909; M. ozzardi cytochrome c oxidase subunit 1, LT623910; M. perstans 12S, LT623913; M. ozzardi 12S, LT623914; M. perstans internal transcribed spacer 1, LT623911; and M. ozzardi internal transcribed spacer 1, LT623912).

Mentions: By using 3 DNA sequences commonly used in the molecular systematics of filarial parasites (the nuclear internal transcribed spacer 1 [ITS1]–based ribosomal DNA sequence [7] and the mitochondrial 12S and cytochrome c oxidase subunit 1 genes [6]), we confirmed M. perstans microfilariae morphologic identifications made using thick blood smears prepared from persons residing in the village of São Gabriel da Cachoeira, Amazonas state, Brazil. Besides providing verification of M. perstans morphologic identifications, the ITS1 sequences generated for this study allowed a phylogenetic analysis with M. perstans from Africa. The ribosomal ITS1 M. perstans from Brazil clustered with other M. perstans ITS1 sequences originating from Africa in a strongly (>94%) bootstrap-supported M. perstans–exclusive monophyletic group (Figure). Similarly, M. ozzardi ITS1 sequences obtained from parasites from Brazil clustered in another strongly (>97%) bootstrap-supported monophyletic group containing only M. ozzardi origin sequences.


Molecular Verification of New World Mansonella perstans Parasitemias
Maximum-likelihood phylogenetic trees showing the relationship between Mansonella parasites from Amazon region of Brazil (Amazonas state) and some of their closest relatives. A) Ribosomal internal transcribed spacer 1–based phylogeny. B) Mitochondrial cytochrome c oxidase subunit 1–based phylogeny. C) Mitochondrial 12S-based phylogeny. All 3 trees were prepared by using DNA sequence alignments and PHYLIP version 3.67 (http://evolution.genetics.washington.edu/phylip.html). Black circles indicate significant bootstrap-supported nodes as a percentage of 1,000 pseudoreplicates. Solid boxes indicate M. perstans and dashed boxes M. ozzardi sequences generated for this study and used in the construction of the displayed trees. Scale bars indicate nucleotide substitutions per site. These sequence have been submitted to GenBank and EMBL (accession nos.: M. perstans cytochrome c oxidase subunit 1, LT623909; M. ozzardi cytochrome c oxidase subunit 1, LT623910; M. perstans 12S, LT623913; M. ozzardi 12S, LT623914; M. perstans internal transcribed spacer 1, LT623911; and M. ozzardi internal transcribed spacer 1, LT623912).
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5382733&req=5

Figure 1: Maximum-likelihood phylogenetic trees showing the relationship between Mansonella parasites from Amazon region of Brazil (Amazonas state) and some of their closest relatives. A) Ribosomal internal transcribed spacer 1–based phylogeny. B) Mitochondrial cytochrome c oxidase subunit 1–based phylogeny. C) Mitochondrial 12S-based phylogeny. All 3 trees were prepared by using DNA sequence alignments and PHYLIP version 3.67 (http://evolution.genetics.washington.edu/phylip.html). Black circles indicate significant bootstrap-supported nodes as a percentage of 1,000 pseudoreplicates. Solid boxes indicate M. perstans and dashed boxes M. ozzardi sequences generated for this study and used in the construction of the displayed trees. Scale bars indicate nucleotide substitutions per site. These sequence have been submitted to GenBank and EMBL (accession nos.: M. perstans cytochrome c oxidase subunit 1, LT623909; M. ozzardi cytochrome c oxidase subunit 1, LT623910; M. perstans 12S, LT623913; M. ozzardi 12S, LT623914; M. perstans internal transcribed spacer 1, LT623911; and M. ozzardi internal transcribed spacer 1, LT623912).
Mentions: By using 3 DNA sequences commonly used in the molecular systematics of filarial parasites (the nuclear internal transcribed spacer 1 [ITS1]–based ribosomal DNA sequence [7] and the mitochondrial 12S and cytochrome c oxidase subunit 1 genes [6]), we confirmed M. perstans microfilariae morphologic identifications made using thick blood smears prepared from persons residing in the village of São Gabriel da Cachoeira, Amazonas state, Brazil. Besides providing verification of M. perstans morphologic identifications, the ITS1 sequences generated for this study allowed a phylogenetic analysis with M. perstans from Africa. The ribosomal ITS1 M. perstans from Brazil clustered with other M. perstans ITS1 sequences originating from Africa in a strongly (>94%) bootstrap-supported M. perstans–exclusive monophyletic group (Figure). Similarly, M. ozzardi ITS1 sequences obtained from parasites from Brazil clustered in another strongly (>97%) bootstrap-supported monophyletic group containing only M. ozzardi origin sequences.

View Article: PubMed Central - PubMed

ABSTRACT

We obtained ribosomal and mitochondrial DNA sequences from residents of Amazonas state, Brazil, with Mansonella parasitemias. Phylogenetic analysis of these sequences confirm that M. ozzardi and M. perstans parasites occur in sympatry and reveal the close relationship between M. perstans in Africa and Brazil, providing insights into the parasite’s New World origins.

No MeSH data available.