Limits...
Mycobacterium chimaera in Heater – Cooler Units in Denmark Related to Isolates from the United States and United Kingdom

View Article: PubMed Central - PubMed

ABSTRACT

Mycobacterium chimaera was present at high rates (>80%) in heater–cooler units (HCUs) from all 5 thoracic surgery departments in Denmark. Isolates were clonal to HCU-associated isolates from the United States (including some from patients) and United Kingdom. However, M. chimaera from 2 brands of HCU were genetically distinct.

No MeSH data available.


Related in: MedlinePlus

Maximum parsimony phylogenies showing the relationship between Mycobacterium chimaera isolates found in patients (circles) and heater–cooler units (HCUs; diamonds) in Denmark. M. chimaera strain FI-0169 (accession no. PRJNA356276) was included for reference. A) Tree showing isolates from Sorin 3T (Sorin Group, Arvada, CO, USA) and Maquet (Maquet, Wayne, NJ, USA) HCUs and a non–HCU-associated isolate from a patient (PAT) in Denmark (DK). B) Phylogenetic tree comparing isolates from Denmark to 31 isolates collected in 3 other countries (Ireland [IRL], United Kingdom, and United States) and retrieved from the European Nucleotide Archive (http://www.ebi.ac.uk/ena). Isolates from Denmark are indicated by open symbols and isolates from other countries by solid symbols. Branch values indicate percentwise bootstrap support (only >70% support is shown), based on 100 replicates. IA, Iowa; PA, Pennsylvania. Scale bars represent a difference of 10 single-nucleotide polymorphisms.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC5382728&req=5

Figure 1: Maximum parsimony phylogenies showing the relationship between Mycobacterium chimaera isolates found in patients (circles) and heater–cooler units (HCUs; diamonds) in Denmark. M. chimaera strain FI-0169 (accession no. PRJNA356276) was included for reference. A) Tree showing isolates from Sorin 3T (Sorin Group, Arvada, CO, USA) and Maquet (Maquet, Wayne, NJ, USA) HCUs and a non–HCU-associated isolate from a patient (PAT) in Denmark (DK). B) Phylogenetic tree comparing isolates from Denmark to 31 isolates collected in 3 other countries (Ireland [IRL], United Kingdom, and United States) and retrieved from the European Nucleotide Archive (http://www.ebi.ac.uk/ena). Isolates from Denmark are indicated by open symbols and isolates from other countries by solid symbols. Branch values indicate percentwise bootstrap support (only >70% support is shown), based on 100 replicates. IA, Iowa; PA, Pennsylvania. Scale bars represent a difference of 10 single-nucleotide polymorphisms.

Mentions: WGS analysis (Figure) showed that the 4 isolates from the Denmark Sorin 3T HCUs were nearly identical (<3 single nucleotide polymorphisms [SNPs]). Conversely, the isolate collected from the Maquet HCU was genetically distinct, showing 47–49 SNP differences compared with the isolates from the Sorin 3T HCUs. The unrelated patient isolate was not closely related to the HCU isolates (30–37 SNP differences).


Mycobacterium chimaera in Heater – Cooler Units in Denmark Related to Isolates from the United States and United Kingdom
Maximum parsimony phylogenies showing the relationship between Mycobacterium chimaera isolates found in patients (circles) and heater–cooler units (HCUs; diamonds) in Denmark. M. chimaera strain FI-0169 (accession no. PRJNA356276) was included for reference. A) Tree showing isolates from Sorin 3T (Sorin Group, Arvada, CO, USA) and Maquet (Maquet, Wayne, NJ, USA) HCUs and a non–HCU-associated isolate from a patient (PAT) in Denmark (DK). B) Phylogenetic tree comparing isolates from Denmark to 31 isolates collected in 3 other countries (Ireland [IRL], United Kingdom, and United States) and retrieved from the European Nucleotide Archive (http://www.ebi.ac.uk/ena). Isolates from Denmark are indicated by open symbols and isolates from other countries by solid symbols. Branch values indicate percentwise bootstrap support (only >70% support is shown), based on 100 replicates. IA, Iowa; PA, Pennsylvania. Scale bars represent a difference of 10 single-nucleotide polymorphisms.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5382728&req=5

Figure 1: Maximum parsimony phylogenies showing the relationship between Mycobacterium chimaera isolates found in patients (circles) and heater–cooler units (HCUs; diamonds) in Denmark. M. chimaera strain FI-0169 (accession no. PRJNA356276) was included for reference. A) Tree showing isolates from Sorin 3T (Sorin Group, Arvada, CO, USA) and Maquet (Maquet, Wayne, NJ, USA) HCUs and a non–HCU-associated isolate from a patient (PAT) in Denmark (DK). B) Phylogenetic tree comparing isolates from Denmark to 31 isolates collected in 3 other countries (Ireland [IRL], United Kingdom, and United States) and retrieved from the European Nucleotide Archive (http://www.ebi.ac.uk/ena). Isolates from Denmark are indicated by open symbols and isolates from other countries by solid symbols. Branch values indicate percentwise bootstrap support (only >70% support is shown), based on 100 replicates. IA, Iowa; PA, Pennsylvania. Scale bars represent a difference of 10 single-nucleotide polymorphisms.
Mentions: WGS analysis (Figure) showed that the 4 isolates from the Denmark Sorin 3T HCUs were nearly identical (<3 single nucleotide polymorphisms [SNPs]). Conversely, the isolate collected from the Maquet HCU was genetically distinct, showing 47–49 SNP differences compared with the isolates from the Sorin 3T HCUs. The unrelated patient isolate was not closely related to the HCU isolates (30–37 SNP differences).

View Article: PubMed Central - PubMed

ABSTRACT

Mycobacterium&nbsp;chimaera was present at high rates (&gt;80%) in heater&ndash;cooler units (HCUs) from all 5 thoracic surgery departments in Denmark. Isolates were clonal to HCU-associated isolates from the United States (including some from patients) and United Kingdom. However, M. chimaera from 2 brands of HCU were genetically distinct.

No MeSH data available.


Related in: MedlinePlus