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Cotton-based Diagnostic Devices

View Article: PubMed Central - PubMed

ABSTRACT

A good diagnostic procedure avoids wasting medical resources, is easy to use, resists contamination, and provides accurate information quickly to allow for rapid follow-up therapies. We developed a novel diagnostic procedure using a “cotton-based diagnostic device” capable of real-time detection, i.e., in vitro diagnostics (IVD), which avoids reagent contamination problems common to existing biomedical devices and achieves the abovementioned goals of economy, efficiency, ease of use, and speed. Our research reinforces the advantages of an easy-to-use, highly accurate diagnostic device created from an inexpensive and readily available U.S. FDA-approved material (i.e., cotton as flow channel and chromatography paper as reaction zone) that adopts a standard calibration curve method in a buffer system (i.e., nitrite, BSA, urobilinogen and uric acid assays) to accurately obtain semi-quantitative information and limit the cross-contamination common to multiple-use tools. Our system, which specifically targets urinalysis diagnostics and employs a multiple biomarker approach, requires no electricity, no professional training, and is exceptionally portable for use in remote or home settings. This could be particularly useful in less industrialized areas.

No MeSH data available.


Related in: MedlinePlus

Schematic diagram of our novel lateral flow cotton-based diagnostic device.This cotton-based device was designed for the diagnosis in a buffer system or in a clinical system. (A) Our cotton-based diagnostic device contains three parts: sample/reaction zone test pad (a single-layer membrane, Whatman chromatography paper), flow channel of cotton hydrophobic and hydrophilic layer (a cotton membrane, Shiseido cleansing cotton), and a background packaging substrate (a thick plastic material lamination film). (B) Top view and cross section show the distance of each test pad (1 cm) and sample flow direction (orange line). (C) This lateral flow-based device has been shown for the diagnosis of BSA, nitrite, and uric acid assays in a buffer system. Photographic evidence of our device test pads (top view) showing negative control and experimental example after we immobilized BSA, nitrite, and uric acid assays on the test pad.
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f1: Schematic diagram of our novel lateral flow cotton-based diagnostic device.This cotton-based device was designed for the diagnosis in a buffer system or in a clinical system. (A) Our cotton-based diagnostic device contains three parts: sample/reaction zone test pad (a single-layer membrane, Whatman chromatography paper), flow channel of cotton hydrophobic and hydrophilic layer (a cotton membrane, Shiseido cleansing cotton), and a background packaging substrate (a thick plastic material lamination film). (B) Top view and cross section show the distance of each test pad (1 cm) and sample flow direction (orange line). (C) This lateral flow-based device has been shown for the diagnosis of BSA, nitrite, and uric acid assays in a buffer system. Photographic evidence of our device test pads (top view) showing negative control and experimental example after we immobilized BSA, nitrite, and uric acid assays on the test pad.

Mentions: A number of items encountered in daily life use cotton in their manufacture, including cleansing cotton, panty liners, and diapers. Further, cotton is U.S. FDA-approved for healthcare uses, in part because it does not cause inflammatory skin responses (e.g., as with tampons or diapers). The function of cotton is often to absorb liquid matter, typically from the skin, making it a natural choice for a biomedical device requiring an absorptive function. However, in the design of our fluid channel, we used a specific kind of cotton consisting of hydrophobic (cotton surface layers) and hydrophilic (cotton interior) properties that are jointly leveraged to create effective channels for sample delivery. For reaction areas and results display, we embedded chromatography paper, our test pad, onto the exterior surface of the cotton via stacking. As liquid sample is added, it flows along hydrophilic cotton layers, and a portion of the liquid sample subsequently flows to the first assay reagent pad then proceeds through that to the second assay pad as shown in Fig. 1. Cross-contamination is avoided, and an accurate multiple-biomarker standard calibration curve is performed immediately. We strongly believe that cotton-based analysis devices have more potential and offer greater plasticity for advanced IVD system development, especially for various point-of-care diagnostic implementations. In summary, this cotton-based diagnostic device supplies a number of benefits including rapid detection time (less than 5 minutes), high sensitivity via the dilution effect provided by cotton channels and the chromatographic effect of paper (chromatography paper) at the test pad point, and avoidance of common contamination issues between reaction areas during fluid delivery while allowing for the adoption of standard calibration curve analyses to accurately obtain semi-quantitative information.


Cotton-based Diagnostic Devices
Schematic diagram of our novel lateral flow cotton-based diagnostic device.This cotton-based device was designed for the diagnosis in a buffer system or in a clinical system. (A) Our cotton-based diagnostic device contains three parts: sample/reaction zone test pad (a single-layer membrane, Whatman chromatography paper), flow channel of cotton hydrophobic and hydrophilic layer (a cotton membrane, Shiseido cleansing cotton), and a background packaging substrate (a thick plastic material lamination film). (B) Top view and cross section show the distance of each test pad (1 cm) and sample flow direction (orange line). (C) This lateral flow-based device has been shown for the diagnosis of BSA, nitrite, and uric acid assays in a buffer system. Photographic evidence of our device test pads (top view) showing negative control and experimental example after we immobilized BSA, nitrite, and uric acid assays on the test pad.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5382709&req=5

f1: Schematic diagram of our novel lateral flow cotton-based diagnostic device.This cotton-based device was designed for the diagnosis in a buffer system or in a clinical system. (A) Our cotton-based diagnostic device contains three parts: sample/reaction zone test pad (a single-layer membrane, Whatman chromatography paper), flow channel of cotton hydrophobic and hydrophilic layer (a cotton membrane, Shiseido cleansing cotton), and a background packaging substrate (a thick plastic material lamination film). (B) Top view and cross section show the distance of each test pad (1 cm) and sample flow direction (orange line). (C) This lateral flow-based device has been shown for the diagnosis of BSA, nitrite, and uric acid assays in a buffer system. Photographic evidence of our device test pads (top view) showing negative control and experimental example after we immobilized BSA, nitrite, and uric acid assays on the test pad.
Mentions: A number of items encountered in daily life use cotton in their manufacture, including cleansing cotton, panty liners, and diapers. Further, cotton is U.S. FDA-approved for healthcare uses, in part because it does not cause inflammatory skin responses (e.g., as with tampons or diapers). The function of cotton is often to absorb liquid matter, typically from the skin, making it a natural choice for a biomedical device requiring an absorptive function. However, in the design of our fluid channel, we used a specific kind of cotton consisting of hydrophobic (cotton surface layers) and hydrophilic (cotton interior) properties that are jointly leveraged to create effective channels for sample delivery. For reaction areas and results display, we embedded chromatography paper, our test pad, onto the exterior surface of the cotton via stacking. As liquid sample is added, it flows along hydrophilic cotton layers, and a portion of the liquid sample subsequently flows to the first assay reagent pad then proceeds through that to the second assay pad as shown in Fig. 1. Cross-contamination is avoided, and an accurate multiple-biomarker standard calibration curve is performed immediately. We strongly believe that cotton-based analysis devices have more potential and offer greater plasticity for advanced IVD system development, especially for various point-of-care diagnostic implementations. In summary, this cotton-based diagnostic device supplies a number of benefits including rapid detection time (less than 5 minutes), high sensitivity via the dilution effect provided by cotton channels and the chromatographic effect of paper (chromatography paper) at the test pad point, and avoidance of common contamination issues between reaction areas during fluid delivery while allowing for the adoption of standard calibration curve analyses to accurately obtain semi-quantitative information.

View Article: PubMed Central - PubMed

ABSTRACT

A good diagnostic procedure avoids wasting medical resources, is easy to use, resists contamination, and provides accurate information quickly to allow for rapid follow-up therapies. We developed a novel diagnostic procedure using a “cotton-based diagnostic device” capable of real-time detection, i.e., in vitro diagnostics (IVD), which avoids reagent contamination problems common to existing biomedical devices and achieves the abovementioned goals of economy, efficiency, ease of use, and speed. Our research reinforces the advantages of an easy-to-use, highly accurate diagnostic device created from an inexpensive and readily available U.S. FDA-approved material (i.e., cotton as flow channel and chromatography paper as reaction zone) that adopts a standard calibration curve method in a buffer system (i.e., nitrite, BSA, urobilinogen and uric acid assays) to accurately obtain semi-quantitative information and limit the cross-contamination common to multiple-use tools. Our system, which specifically targets urinalysis diagnostics and employs a multiple biomarker approach, requires no electricity, no professional training, and is exceptionally portable for use in remote or home settings. This could be particularly useful in less industrialized areas.

No MeSH data available.


Related in: MedlinePlus